Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,659,909 T→C 8.9% intergenic (‑44/+31) PP_3223 ← / ← PP_3224 ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,659,9090TC 8.9% 148.5 / 5.1 56intergenic (‑44/+31)PP_3223/PP_3224ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base C (2/3);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

AGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAC  >  NC_002947/3659771‑3660050
                                                                                                                                          |                                                                                                                                             
aGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAAc                                                                                                                                     <  2:268876/149‑1 (MQ=255)
          tGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAggaga                                                                                                                               >  8:47116/1‑142 (MQ=255)
          tGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAggaga                                                                                                                               <  7:47116/145‑4 (MQ=255)
          tGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAgg                                                                                                                           >  2:61759/1‑149 (MQ=255)
            gTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAgg                                                                                                                           <  1:61759/147‑1 (MQ=255)
            gTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTg                                                                                                                         >  6:268303/1‑149 (MQ=255)
             ttGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGc                                                                                                                        <  2:245212/149‑1 (MQ=255)
                aGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAg                                                                                                                            <  2:29747/142‑1 (MQ=255)
                aGGTGTTGTCGGCCGGCGAGCAAGCGGTGACGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAg                                                                                                                            >  1:29747/1‑142 (MQ=255)
                 ggTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCg                                                                                                                                         >  8:81619/1‑128 (MQ=255)
                 ggTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCg                                                                                                                                         <  7:81619/128‑1 (MQ=255)
                    gttgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCGCCTGTTCCGGCGAACTGGCCGGAAc                                                                                                                                     >  4:47614/1‑129 (MQ=255)
                    gttgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCGCCTGTTCCGGCGAACTGGCCGGAAc                                                                                                                                     <  3:47614/129‑1 (MQ=255)
                     ttgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagt                                                                                                                <  8:130909/149‑1 (MQ=255)
                          ggccggcGAGCATGCGGTGAGGCCCAGGGCCAGGGTGCCTATCGTTATTTGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtcagtc                                                                                                           <  1:299980/149‑1 (MQ=255)
                          ggccggcGAGCAAGCGGTGAGGCCCAGGGCCATGGTGCCGATAGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTACTGTTCCTGAAAGTTCGACGGAACAGGCAAAAGGTGCCTTGTagtcagtc                                                                                                           >  5:367547/1‑149 (MQ=255)
                           gccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTcc                                                                                                          >  8:256257/1‑149 (MQ=255)
                             cgagctgCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGGGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTg                                                                                                        <  7:227913/143‑1 (MQ=255)
                                    cAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTTTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTg                                                                                                        >  8:227913/1‑142 (MQ=255)
                                    cAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCg                                                                                                 <  5:268303/149‑1 (MQ=255)
                                       gCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAgg                                                                                              >  5:322646/1‑149 (MQ=255)
                                         ggTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGcc                                                                                            <  8:220154/149‑1 (MQ=255)
                                          gTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGccc                                                                                           >  1:315749/1‑149 (MQ=255)
                                             aGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTcg                                                                                        <  7:256257/149‑1 (MQ=255)
                                               gCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACa                                                                                                                                    <  4:189908/103‑1 (MQ=255)
                                               gCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACa                                                                                                                                    >  3:189908/1‑103 (MQ=255)
                                                 ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGAt                                                                                    >  6:11335/1‑149 (MQ=255)
                                                  cagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATc                                                                                   <  6:322646/149‑1 (MQ=255)
                                                     ggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCCGGCCTGGTAATCGAGGCCCTCGCGATccca                                                                                >  6:5346/1‑147 (MQ=255)
                                                      gccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGc                                                                                             <  8:100398/135‑1 (MQ=255)
                                                      gccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGc                                                                                             >  7:100398/1‑135 (MQ=255)
                                                       ccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGa                                                                                     >  8:12696/1‑142 (MQ=255)
                                                       ccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGa                                                                                     <  7:12696/142‑1 (MQ=255)
                                                                cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCa                                                                     <  7:169037/149‑1 (MQ=255)
                                                                      cgAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAAt                                                                       >  8:1483/1‑141 (MQ=255)
                                                                      cgAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAAt                                                                       <  7:1483/141‑1 (MQ=255)
                                                                             ttGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATg                                                        <  2:315749/149‑1 (MQ=255)
                                                                                 tGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGt                                                    >  6:180152/1‑149 (MQ=255)
                                                                                   cattGGGGGAATGTGATTTATCCTTCAATTAATGTTGATCTCCTGTTCCGGCCAGTTCGCCGGAACATGCAAAAGGAGCCTTTGATTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCAGAATCAGGTCATCCCAATTCCGtt                                                   <  6:367547/145‑1 (MQ=255)
                                                                                            cATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTc                                         <  5:180152/149‑1 (MQ=255)
                                                                                             ttGGGATTGATCATTCAATGATTGGGGCTATCCTGTTCCGGCCAGTTCGACGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCACTCGCGATCCAACTGcc                                                                           <  4:328376/113‑1 (MQ=255)
                                                                                             aTGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGCCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGcc                                                                           >  3:328376/1‑114 (MQ=255)
                                                                                                 gATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCt                                    >  8:45805/1‑149 (MQ=255)
                                                                                                      aTCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCa                               >  8:49604/1‑149 (MQ=255)
                                                                                                         cTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                            >  1:25388/1‑149 (MQ=255)
                                                                                                            cAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGtt                                                   >  4:202775/1‑123 (MQ=255)
                                                                                                            cAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGtt                                                   <  3:202775/123‑1 (MQ=255)
                                                                                                              atgaatgGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGaaaa                       >  1:206407/1‑149 (MQ=255)
                                                                                                                           tcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGc          >  6:55211/1‑149 (MQ=255)
                                                                                                                             cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGTTCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCt         <  1:312928/148‑1 (MQ=255)
                                                                                                                             cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAACACCGCTTTGACTGCt         >  2:69907/1‑148 (MQ=255)
                                                                                                                             cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGc          <  5:11335/147‑1 (MQ=255)
                                                                                                                              tGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTg                                   <  5:206751/121‑1 (MQ=255)
                                                                                                                              tGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTg                                   >  6:206751/1‑121 (MQ=255)
                                                                                                                                ttCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATg     >  8:228891/1‑149 (MQ=255)
                                                                                                                                   cGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAc  <  7:45805/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                             
AGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAC  >  NC_002947/3659771‑3660050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑