Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,779,131 A→G 8.3% L111S (TTG→TCG) ‡ nikR ← nickel‑responsive regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,779,1310AG 8.3% 192.7 / 4.8 71L111S (TTG→TCG) ‡nikRnickel‑responsive regulator
Reads supporting (aligned to +/- strand):  ref base A (37/28);  new base G (3/3);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTGCTGTG  >  NC_002947/3778989‑3779279
                                                                                                                                              |                                                                                                                                                    
ggACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGc                                                                                                                                                >  1:279782/1‑149 (MQ=255)
    ttCTGAGTCACGGCCCTTGGGCCGATCCCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCAGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGAGCTCAACCATCAATTGGAGAGACAACTCGCCAAt                                                                                                                                            >  4:265644/1‑149 (MQ=255)
    ttCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAAt                                                                                                                                            >  5:21249/1‑149 (MQ=255)
      cTGTGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGTCGCAACGCCTCATCGCTCCTTCGGCTTGCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAAtt                                                                                                                                           <  6:324114/148‑1 (MQ=255)
         aGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCgg                                                                                                                                        <  2:279782/148‑1 (MQ=255)
            aaCGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGc                                                                                                                                    >  8:138677/1‑149 (MQ=255)
                gCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTc                                                                                                                                >  4:114817/1‑149 (MQ=255)
                      gggCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGccc                                                                                                                            <  8:187462/147‑1 (MQ=255)
                               gcgTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCga                                                                                                                 <  3:114817/149‑1 (MQ=255)
                                   cACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGACCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGATAcc                                                                                                             >  6:91352/1‑149 (MQ=255)
                                      gAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGTTGTCTCGCCAAttgat                                                                                                                                        <  7:269508/119‑4 (MQ=255)
                                      gAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGTTGTCTCGCCAAttgat                                                                                                                                        >  8:269508/1‑116 (MQ=255)
                                       aaCAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCGACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTc                                                                                                           >  8:162607/1‑147 (MQ=255)
                                       aaCAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTc                                                                                                           >  3:191032/1‑147 (MQ=255)
                                         cAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAgg                                                                                                       <  7:196082/149‑1 (MQ=255)
                                          aaGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGcc                                                                                                                             >  7:77363/1‑126 (MQ=255)
                                          aaGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGcc                                                                                                                             <  8:77363/126‑1 (MQ=255)
                                            gTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACAGAGACCTCCAGGCAg                                                                                                    >  8:337899/1‑149 (MQ=255)
                                               aGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGcc                                                                                                                             <  5:365061/121‑1 (MQ=255)
                                               aGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGcc                                                                                                                             >  6:365061/1‑121 (MQ=255)
                                                 gCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCa                                                                                                                                              <  3:300414/102‑1 (MQ=255)
                                                 gCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCa                                                                                                                                              >  4:300414/1‑102 (MQ=255)
                                                   aaCGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCAGCTGGCCATGTTCCCCGCCGCGCTCCACCATCAATTGGCGAGACAACTCGGGAATTCGGCCGCGTTCGCCCTGCCGCCCCGAGCCCTCCGGGCGGGGGGCgg                                                                                             >  6:265827/1‑149 (MQ=255)
                                                     cGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCa                                                                                           <  3:250167/149‑1 (MQ=255)
                                                       ccTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATc                                                                                         <  4:191032/149‑1 (MQ=255)
                                                         tCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATc                                                                                         >  6:231996/1‑147 (MQ=255)
                                                               cACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGca                                                                                 >  5:205180/1‑149 (MQ=255)
                                                               cACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGca                                                                                 >  5:200247/1‑149 (MQ=255)
                                                                aCCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGcac                                                                                <  6:205180/149‑1 (MQ=255)
                                                                aCCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGcac                                                                                <  6:200247/149‑1 (MQ=255)
                                                                   ttCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATg                                                                             >  8:296406/1‑149 (MQ=255)
                                                                     cGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATg                                                                             <  5:231996/147‑1 (MQ=255)
                                                                      ggCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAg                                                                          >  2:166518/1‑149 (MQ=255)
                                                                           ccccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGt                                                                       <  7:296406/147‑1 (MQ=255)
                                                                           ccccGGAGCCGGGAGCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTgg                                                                     >  6:204766/1‑149 (MQ=255)
                                                                             ccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGcaca                                                                               <  6:34879/137‑1 (MQ=255)
                                                                             ccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGcaca                                                                               >  5:34879/1‑137 (MQ=255)
                                                                             ccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAt                                                                   >  5:218325/1‑149 (MQ=255)
                                                                             ccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGAGCACATGCAGGGTGGAt                                                                   >  3:49385/1‑149 (MQ=255)
                                                                                gAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCCATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCGCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCgg                                                                >  6:162317/1‑149 (MQ=255)
                                                                                   ccGGGATCACCTGCCCCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCCCCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGt                                                              >  7:319347/1‑148 (MQ=255)
                                                                                   ccGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTc                                                             <  7:138677/149‑1 (MQ=255)
                                                                                         tCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGt                                                              >  4:251621/1‑142 (MQ=255)
                                                                                          cacctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGcac                                                                                >  2:359440/1‑123 (MQ=255)
                                                                                          cacctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGcac                                                                                <  1:359440/123‑1 (MQ=255)
                                                                                               gcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGtggtgg                                                 >  4:120632/1‑149 (MQ=255)
                                                                                                 acctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGAGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGc                                               <  4:49385/149‑1 (MQ=255)
                                                                                                   ctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTg                                                                      <  3:158369/124‑1 (MQ=255)
                                                                                                   ctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTg                                                                      >  4:158369/1‑124 (MQ=255)
                                                                                                   ctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGt                                             >  2:206463/1‑149 (MQ=255)
                                                                                                   ctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGAGAGACAATTCGACAATTCGGCCGCTTTCGCCCTGCAGCACAGAGACCTCCAGGCAGTGGCCGGCATCAAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGt                                             >  8:172689/1‑149 (MQ=255)
                                                                                                            ttCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCt                                    >  8:29046/1‑149 (MQ=255)
                                                                                                              ccACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTgg                                  >  7:119336/1‑149 (MQ=255)
                                                                                                              ccACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTg                                   <  6:218325/148‑1 (MQ=255)
                                                                                                               cACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCAGCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTgg                                  <  7:337899/148‑1 (MQ=255)
                                                                                                                aCGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGtt                                <  3:120632/149‑1 (MQ=255)
                                                                                                                  gccgcGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGtt                                >  1:330766/1‑147 (MQ=255)
                                                                                                                     gcgcTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGcc                           <  8:119336/149‑1 (MQ=255)
                                                                                                                       gcTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGcc                           <  3:265644/147‑1 (MQ=255)
                                                                                                                         tCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCt                       >  4:202782/1‑149 (MQ=255)
                                                                                                                           aaCCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTgg                     <  1:166518/149‑1 (MQ=255)
                                                                                                                              cATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCa                  <  3:202782/149‑1 (MQ=255)
                                                                                                                               ccgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTgg                                  >  7:329997/4‑132 (MQ=255)
                                                                                                                               ccgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTgg                                  <  8:329997/129‑1 (MQ=255)
                                                                                                                               aTCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGTATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAg                 >  6:321878/1‑149 (MQ=255)
                                                                                                                                cgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGt                >  6:310768/3‑149 (MQ=255)
                                                                                                                                cgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGACACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGc                                           <  6:285976/120‑1 (MQ=255)
                                                                                                                                    ttGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCATATCGTGGTGGGCGTGCAGGGTCTGGTTGa                              <  2:331243/131‑1 (MQ=255)
                                                                                                                                    ttGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGa                              >  1:331243/1‑131 (MQ=255)
                                                                                                                                    ttGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTcgc            <  7:162607/149‑1 (MQ=255)
                                                                                                                                            aCAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTGCtg    >  5:64046/1‑149 (MQ=255)
                                                                                                                                              aaCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTGCtgtg  <  6:64046/149‑1 (MQ=255)
                                                                                                                                              aaCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTGCtgtg  >  4:125401/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTGCTGTG  >  NC_002947/3778989‑3779279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑