Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,848,779 G→T 10.6% V182F (GTC→TTC) ‡ PP_3397 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,848,7790GT10.6% 116.0 / 2.7 42V182F (GTC→TTC) ‡PP_3397hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/15);  new base T (2/2);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GAGGTCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGTCAAC  >  NC_002947/3848632‑3848922
                                                                                                                                                   |                                                                                                                                                
gAGGTCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGt                                                                                                                                                 >  2:297802/1‑149 (MQ=255)
    tCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAAc                                                                                                                                             <  1:297802/149‑1 (MQ=255)
      tACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGt                                                                                                                                           >  3:173533/1‑149 (MQ=255)
      tACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGt                                                                                                                                           >  6:312233/1‑149 (MQ=255)
         ggtGCCGGTATAGAGGCAGCCGTTGCTGCTGAAAACCCGGTAGCCGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGTTACCTACACCATCACGCACCCTTACGGGGTGGTGACGTTGACCGTctc                                                                                                                                        <  6:352639/147‑3 (MQ=255)
              cGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGTGACGTTGACCGTctccaccc                                                                                                                                   <  2:350326/149‑3 (MQ=255)
              cGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGCTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACCCACCCGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCACCGTCCCCCCgg                                                                                                                                   >  7:204043/1‑149 (MQ=255)
                   taGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGgt                                                                                                                              >  6:222777/1‑149 (MQ=255)
                            ccTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTcgcgcg                                                                                                                     <  7:190727/149‑1 (MQ=255)
                                      tgAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCa                                                                                                           >  5:237984/1‑149 (MQ=255)
                                       gAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCAc                                                                                                          >  6:295411/1‑149 (MQ=255)
                                        aaaaCCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGAGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCAc                                                                                                          <  4:345183/148‑1 (MQ=255)
                                                         gATCACCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGAAAGGTCGACGCGCGATAAACATCACCCGGGACATCGGTAT‑Cgg                                                                                       >  3:281610/1‑149 (MQ=255)
                                                              gCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTGACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCTCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTc                                                                                  >  4:8178/1‑149 (MQ=255)
                                                               cAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCCCCCGGGACCTCGGGAT‑CGGCGCTcc                                                                                 >  8:267628/1‑149 (MQ=255)
                                                                   gTTTCGCCCGCATCCGTATGCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACTGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGc                                                                             <  5:186042/149‑1 (MQ=255)
                                                                         cccGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTc                                                                       >  8:47668/1‑149 (MQ=255)
                                                                           cGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGc                                                                             <  8:219913/141‑1 (MQ=255)
                                                                           cGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGc                                                                             >  7:219913/1‑141 (MQ=255)
                                                                             cATCCGTATCCGCGCATCGGTCTCACGCACCGGTAAATACACAATCACGCACACTTACGGGATAGAAACGATAAACATCACCACGGAAGGTCGTCGCGCGATAAACATAACCCGGGAAAAAGGTAT‑CGGAGCTCCGGGCAAAATCCAgg                                                                   >  7:54054/1‑149 (MQ=255)
                                                                                       cgcgCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAg                                                                    <  4:300047/138‑1 (MQ=255)
                                                                                       cgcgCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAg                                                                    >  3:300047/1‑138 (MQ=255)
                                                                                        gcgcATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCCCCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCg                                                        >  5:157241/1‑149 (MQ=255)
                                                                                        gcgcATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCg                                                        >  1:50153/1‑149 (MQ=255)
                                                                                           cATCGGTCCCACGGACCGGTACCTACACCATCCCCCACCCGTACGGGGTGGAGTCGGTCAACGTCACCACGGGATGTCGTCGCGCGTTCAACATCACCCGGGACATCGGTATACGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCggg                                                      <  1:240793/149‑1 (MQ=255)
                                                                                            aTCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCggggg                                                    <  5:295411/149‑1 (MQ=255)
                                                                                                     aCGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGcc                                           >  3:178110/1‑149 (MQ=255)
                                                                                                        cACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCtt                                        <  6:157241/149‑1 (MQ=255)
                                                                                                        cACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCtt                                        <  4:173533/149‑1 (MQ=255)
                                                                                                           cGGTACCTACACCATCACGCACCCTTTCGGGGTGGTGACGGTCAACGTCACCACGGCAGGGCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGGCCTTGGc                                     <  8:204043/149‑1 (MQ=255)
                                                                                                           cGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGc                                     >  4:154939/1‑149 (MQ=255)
                                                                                                                ccTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGaa                                 <  5:222777/148‑1 (MQ=255)
                                                                                                                 cTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATc                                                                                                >  2:210045/1‑85 (MQ=255)
                                                                                                                   aaacCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGAGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCCGGCAACTTCCAGGGGTCGCAGGCGGGGGGGATCGGCCCATGGCTGAAgggg                             >  8:127114/3‑149 (MQ=255)
                                                                                                                      ccATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGtt                          <  5:312233/149‑1 (MQ=255)
                                                                                                                          cACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGTGGGGTcgcg                      >  4:14496/1‑149 (MQ=255)
                                                                                                                               aCCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGt                           >  4:254312/1‑139 (MQ=255)
                                                                                                                               aCCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGt                           <  3:254312/139‑1 (MQ=255)
                                                                                                                                        gggTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCGTGGCTGAAGGGGGTTGGCGGGCCATATACagag        <  2:50153/149‑1 (MQ=255)
                                                                                                                                        ccgtggtgACGTTGACCGTCTCCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACagag        >  5:275081/3‑149 (MQ=255)
                                                                                                                                          gtggtgACGTTGACCGTCTCCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGt      >  7:312228/1‑149 (MQ=255)
                                                                                                                                              agaCGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCACCTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGTCAAc  >  3:270127/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GAGGTCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGTCAAC  >  NC_002947/3848632‑3848922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑