BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,848,783	A→C	11.9%	N183T (AAC→ACC)	PP_3397 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,848,783	0	A	C	11.9%	98.1 / 6.1	41	N183T (AAC→ACC)	PP_3397	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (21/15); new base C (3/2); total (24/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGTCAAC  >  NC_002947/3848636‑3848922

tCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAAc                                                                                                                                             <  1:297802/149‑1 (MQ=255)

  tACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGt                                                                                                                                           >  3:173533/1‑149 (MQ=255)

  tACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGt                                                                                                                                           >  6:312233/1‑149 (MQ=255)

     ggtGCCGGTATAGAGGCAGCCGTTGCTGCTGAAAACCCGGTAGCCGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGTTACCTACACCATCACGCACCCTTACGGGGTGGTGACGTTGACCGTctc                                                                                                                                        <  6:352639/147‑3 (MQ=255)

          cGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGTGACGTTGACCGTctccaccc                                                                                                                                   <  2:350326/149‑3 (MQ=255)

          cGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGCTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACCCACCCGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCACCGTCCCCCCgg                                                                                                                                   >  7:204043/1‑149 (MQ=255)

               taGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGgt                                                                                                                              >  6:222777/1‑149 (MQ=255)

                        ccTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTcgcgcg                                                                                                                     <  7:190727/149‑1 (MQ=255)

                                  tgAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCa                                                                                                           >  5:237984/1‑149 (MQ=255)

                                   gAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCAc                                                                                                          >  6:295411/1‑149 (MQ=255)

                                    aaaaCCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGAGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCAc                                                                                                          <  4:345183/148‑1 (MQ=255)

                                                     gATCACCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGAAAGGTCGACGCGCGATAAACATCACCCGGGACATCGGTAT‑Cgg                                                                                       >  3:281610/1‑149 (MQ=255)

                                                          gCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTGACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCTCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTc                                                                                  >  4:8178/1‑149 (MQ=255)

                                                           cAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCCCCCGGGACCTCGGGAT‑CGGCGCTcc                                                                                 >  8:267628/1‑149 (MQ=255)

                                                               gTTTCGCCCGCATCCGTATGCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACTGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGc                                                                             <  5:186042/149‑1 (MQ=255)

                                                                     cccGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTc                                                                       >  8:47668/1‑149 (MQ=255)

                                                                       cGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGc                                                                             <  8:219913/141‑1 (MQ=255)

                                                                       cGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGc                                                                             >  7:219913/1‑141 (MQ=255)

                                                                         cATCCGTATCCGCGCATCGGTCTCACGCACCGGTAAATACACAATCACGCACACTTACGGGATAGAAACGATAAACATCACCACGGAAGGTCGTCGCGCGATAAACATAACCCGGGAAAAAGGTAT‑CGGAGCTCCGGGCAAAATCCAgg                                                                   >  7:54054/1‑149 (MQ=255)

                                                                                   cgcgCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAg                                                                    <  4:300047/138‑1 (MQ=255)

                                                                                   cgcgCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAg                                                                    >  3:300047/1‑138 (MQ=255)

                                                                                    gcgcATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCCCCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCg                                                        >  5:157241/1‑149 (MQ=255)

                                                                                    gcgcATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCg                                                        >  1:50153/1‑149 (MQ=255)

                                                                                       cATCGGTCCCACGGACCGGTACCTACACCATCCCCCACCCGTACGGGGTGGAGTCGGTCAACGTCACCACGGGATGTCGTCGCGCGTTCAACATCACCCGGGACATCGGTATACGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCggg                                                      <  1:240793/149‑1 (MQ=255)

                                                                                        aTCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCggggg                                                    <  5:295411/149‑1 (MQ=255)

                                                                                                 aCGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGcc                                           >  3:178110/1‑149 (MQ=255)

                                                                                                    cACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCtt                                        <  6:157241/149‑1 (MQ=255)

                                                                                                    cACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCtt                                        <  4:173533/149‑1 (MQ=255)

                                                                                                       cGGTACCTACACCATCACGCACCCTTTCGGGGTGGTGACGGTCAACGTCACCACGGCAGGGCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGGCCTTGGc                                     <  8:204043/149‑1 (MQ=255)

                                                                                                       cGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGc                                     >  4:154939/1‑149 (MQ=255)

                                                                                                            ccTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGaa                                 <  5:222777/148‑1 (MQ=255)

                                                                                                             cTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATc                                                                                                >  2:210045/1‑85 (MQ=255)

                                                                                                               aaacCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGAGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCCGGCAACTTCCAGGGGTCGCAGGCGGGGGGGATCGGCCCATGGCTGAAgggg                             >  8:127114/3‑149 (MQ=255)

                                                                                                                  ccATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGtt                          <  5:312233/149‑1 (MQ=255)

                                                                                                                      cACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGTGGGGTcgcg                      >  4:14496/1‑149 (MQ=255)

                                                                                                                           aCCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGt                           >  4:254312/1‑139 (MQ=255)

                                                                                                                           aCCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGt                           <  3:254312/139‑1 (MQ=255)

                                                                                                                                    gggTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCGTGGCTGAAGGGGGTTGGCGGGCCATATACagag        <  2:50153/149‑1 (MQ=255)

                                                                                                                                    ccgtggtgACGTTGACCGTCTCCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACagag        >  5:275081/3‑149 (MQ=255)

                                                                                                                                      gtggtgACGTTGACCGTCTCCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGt      >  7:312228/1‑149 (MQ=255)

                                                                                                                                          agaCGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCACCTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGTCAAc  >  3:270127/1‑149 (MQ=255)

TCTACGTTGCCGGTATAGAGGCAGCCTTTGCTGCTGAAAACCCGGTAGACGGTGATCAGCAAAGTTTCGCCCGCATCCGTATCCGCGCATCGGTCCCACGCACCGGTACCTACACCATCACGCACCCTTACGGGGTGGAGACGGTCAACGTCACCACGGCAGGTCGTCGCGCGATCAACATCACCCGGGACATCGGTAT‑CGGCGCTCCGGGCAACTTCCAGGGGGCGCTGGCGGGGGCGATCGGCCCTTGGCTGAAGGGGGTTGGCGGGCCATATACAGAGGTCAAC  >  NC_002947/3848636‑3848922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`