Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,097,945 C→G 6.8% V249L (GTT→CTT) ‡ qor‑II ← quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,097,9450CG 6.8% 168.7 / 2.1 56V249L (GTT→CTT) ‡qor‑IIquinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCCGAGGCTGGACGATACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACACCTTGGCCTTGGGTCAGCTCACG  >  NC_002947/4097805‑4098089
                                                                                                                                            |                                                                                                                                                
gACCCGAGGCTGGACGATACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATgg                                                                                                                                          <  3:53453/149‑1 (MQ=255)
   ccGAGGCTGGACGATACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAg                                                                                                                                       >  7:287152/1‑149 (MQ=255)
         cTGGACGATACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTg                                                                                                                                 <  3:259642/149‑1 (MQ=255)
          tGGACGATACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTg                                                                                                                                 >  4:170137/1‑148 (MQ=255)
                aTACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGGGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGa                                                                                                                          <  2:318333/149‑1 (MQ=255)
                 tACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAg                                                                                                                         >  1:108593/1‑149 (MQ=255)
                   ccTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGt                                                                                                                       <  3:170137/149‑1 (MQ=255)
                          cAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTgccgcc                                                                                                                 >  4:56527/1‑148 (MQ=255)
                          cAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTgccgcc                                                                                                                 >  2:196159/1‑148 (MQ=255)
                           aaCCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGCCATTGAGGTTGCCACGCTCAATGCCaatggc                                                                                                                                   <  6:323404/129‑6 (MQ=38)
                           aaCCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGCCATTGAGGTTGCCACGCTCAATGCCaatggc                                                                                                                                   >  5:323404/1‑124 (MQ=38)
                             ccGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAAc                                                                                                             >  1:353736/1‑149 (MQ=255)
                                cTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGCGTCAGGTACAGCGAGCCATAGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGa                                                                                                           >  3:205644/1‑148 (MQ=255)
                                    cAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATAcc                                                                                                       >  1:155241/1‑148 (MQ=255)
                                                  cgcgcTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCAcc                                                                                        <  3:91103/149‑1 (MQ=255)
                                                        gctgGTACTCCTCGACAGTGCGGGTGTGTGCTGGGAGGGATGGCCGGGTCAGGTTCAGCGTGCCTTTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAgg                                                                                  <  4:205644/149‑1 (MQ=255)
                                                         ctgGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAgg                                                                                  <  8:215881/148‑1 (MQ=255)
                                                           ggTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTg                                                                               >  1:315195/1‑149 (MQ=255)
                                                            gTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATa                                                                                                         <  7:324466/122‑1 (MQ=255)
                                                            gTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATa                                                                                                         >  8:324466/1‑122 (MQ=255)
                                                             gacgcctcGACAGTGCGGGGGTGTGCGGCGAGCGATGGCCGGGGCAGGTACAGCGAGCCATGGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGcc                                                                             <  6:104/145‑1 (MQ=255)
                                                              aCTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCTGAACGATACCAACAAGCCGCGCACCCTCAGGCTGcc                                                                             >  5:287614/1‑148 (MQ=255)
                                                                  ctcGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGTGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAgg                                                                                  <  4:63771/139‑1 (MQ=255)
                                                                  ctcGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGTGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAgg                                                                                  >  3:63771/1‑139 (MQ=255)
                                                                  ctcGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGAt                                                                                                          <  6:241909/115‑1 (MQ=255)
                                                                  ctcGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGAt                                                                                                          >  5:241909/1‑115 (MQ=255)
                                                                    cGACAGGGCGGGTGTGTGCTGCGAGGGATGGCCGGGTCTGGTACAGCGAGCCATTGGCATTGTGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGTGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAg                                                                      <  4:100368/149‑1 (MQ=255)
                                                                     gACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGa                                                                       <  3:56527/147‑1 (MQ=255)
                                                                            cGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAg                                                              <  1:196159/149‑1 (MQ=255)
                                                                                   gtgCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACAcc                                                       <  2:155241/149‑1 (MQ=255)
                                                                                         cgagcgaTGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGcc                                                  <  2:353736/148‑1 (MQ=255)
                                                                                          gagcgaTGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCCGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCa                                                >  2:111262/1‑149 (MQ=255)
                                                                                                tGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCTGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAAt                                          <  6:287614/149‑1 (MQ=255)
                                                                                                    cGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGTACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTa                                      <  1:327604/149‑1 (MQ=255)
                                                                                                    cGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGTACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTa                                      >  2:327604/1‑149 (MQ=255)
                                                                                                    cGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCaacc                                                             >  6:127258/1‑124 (MQ=255)
                                                                                                    cGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGc                                                             <  5:127258/126‑1 (MQ=255)
                                                                                                        tCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTacac                                   >  6:121938/1‑148 (MQ=255)
                                                                                                            gTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACgg                              <  5:121938/149‑1 (MQ=255)
                                                                                                            gTACAGCGAGCCATCGGCATTGAGCGTGGCAACCCCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACgg                              >  8:330281/1‑149 (MQ=255)
                                                                                                             tACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGc                             <  8:287152/149‑1 (MQ=255)
                                                                                                              aCAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGc                             >  5:251008/1‑148 (MQ=255)
                                                                                                                cgCGAGCCATTCGCATTGAGCGGGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCcac                          <  6:251008/148‑1 (MQ=255)
                                                                                                                 gCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCcaca                         >  3:260819/1‑149 (MQ=255)
                                                                                                                   gAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAgg                                                                                  >  7:225744/1‑90 (MQ=255)
                                                                                                                   gAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAgg                                                                                  <  8:225744/90‑1 (MQ=255)
                                                                                                                      ccATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACAccttg                    <  4:260819/149‑1 (MQ=255)
                                                                                                                           gacATTGAGGTTGCCACGCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACAccttggcctt               >  4:139941/3‑149 (MQ=255)
                                                                                                                            gCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACAccttggccttg              >  6:158068/1‑149 (MQ=255)
                                                                                                                            gCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACAccttggccttg              >  2:152717/1‑149 (MQ=255)
                                                                                                                             cATTGAGGTTGCCACGCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACAccttggccttgg             <  3:139941/149‑1 (MQ=255)
                                                                                                                             cATTGAGCGTGGCAACCCCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACAccttggccttgg             <  7:330281/149‑1 (MQ=255)
                                                                                                                               ttGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACACCTTGGCCTTggg            >  8:197143/1‑148 (MQ=255)
                                                                                                                                        gCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAAGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACACCTTGGCCTTGGGTCAGCTCACg  >  7:112460/1‑149 (MQ=255)
                                                                                                                                         cAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCcgcg                                                                                            <  7:190845/58‑1 (MQ=255)
                                                                                                                                         cAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCcgcg                                                                                            >  8:190845/1‑58 (MQ=255)
                                                                                                                                            |                                                                                                                                                
GACCCGAGGCTGGACGATACCTTCGGCAACCGCTGCCAGCACATCGGCCGCGCGCTGCTGGTACTCCTCGACAGTGCGGGTGTGTGCTGCGAGCGATGGCCGGGTCAGGTACAGCGAGCCATTGGCATTGAGCGTGGCAACCTCAATGGCAGGCGGTGCACCGGAGGTGCCGCCGAACGATACCAACAAGCCGCGCACCCTCAGGCTGCCCAACGAGGCCTCAAGCGACACCTTGCCCACGCAATCGTACACTACGGCCACACCTTGGCCTTGGGTCAGCTCACG  >  NC_002947/4097805‑4098089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑