Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,126,201 G→A 8.0% A387V (GCG→GTG)  opdT‑II ← tyrosine‑specific outer membrane porin D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,126,2010GA 8.0% 139.5 / 2.2 50A387V (GCG→GTG) opdT‑IItyrosine‑specific outer membrane porin D
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base A (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

CGTCCAGATCCGACTCGAACGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATAACGCAGCATCCACGAACGCTCCTTGGGC  >  NC_002947/4126058‑4126347
                                                                                                                                               |                                                                                                                                                  
cGTCCAGATCCGACTCGAACGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACg                                                                                                                                               >  2:132172/1‑149 (MQ=255)
          cGACTCGAACGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGgcgc                                                                                                                                      <  2:311015/148‑1 (MQ=255)
           gACTCGAACGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCACAACGTCACGGCGCAt                                                                                                                                    >  8:317071/1‑149 (MQ=255)
              tCGAACGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATg                                                                                                                                   >  7:126940/1‑147 (MQ=255)
                 aaCGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGt                                                                                                                              >  2:360430/1‑149 (MQ=255)
                       gTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGtt                                                                                                                        >  1:140678/1‑149 (MQ=255)
                       gTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGtt                                                                                                                        <  1:334878/149‑1 (MQ=255)
                          gCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTg                                                                                                                       >  8:211113/1‑147 (MQ=255)
                          gCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTg                                                                                                                       <  7:211113/147‑1 (MQ=255)
                              gggTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTg                                                                                                                 <  8:240247/149‑1 (MQ=255)
                              gggTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTg                                                                                                                 <  8:155712/149‑1 (MQ=255)
                                gtggtgGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGa                                                                                                               <  2:130161/149‑1 (MQ=255)
                                 tggtggCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGag                                                                                                              <  4:223369/149‑1 (MQ=255)
                                             gAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCg                                                                                                  >  4:73833/1‑149 (MQ=255)
                                                 ccGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTAc                                                                                              >  5:299434/1‑149 (MQ=255)
                                                  cGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTAcc                                                                                             <  6:65269/149‑1 (MQ=255)
                                                       cGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCACAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGc                                                                                        <  7:317071/149‑1 (MQ=255)
                                                              tCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAgcg                                                                                 >  3:44311/1‑149 (MQ=255)
                                                                   ggCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTgg                                                                                                                      <  3:162797/107‑1 (MQ=255)
                                                                   ggCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTgg                                                                                                                      >  4:162797/1‑107 (MQ=255)
                                                                    gCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCACAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCAt                                                                           >  8:93818/1‑149 (MQ=255)
                                                                     ccGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATg                                                                          <  3:73833/149‑1 (MQ=255)
                                                                        cgcCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAAc                                                                       <  6:28562/149‑1 (MQ=255)
                                                                          cccGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGa                                                                     <  6:299434/149‑1 (MQ=255)
                                                                          cccGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGa                                                                     <  7:80058/149‑1 (MQ=255)
                                                                            cGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCAt                                                                           <  1:325070/141‑1 (MQ=255)
                                                                            cGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCAt                                                                           >  2:325070/1‑141 (MQ=255)
                                                                                    ccacATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCACAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGt                                                           <  7:93818/149‑1 (MQ=255)
                                                                                      acaTACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAg                                                                                                                                <  8:124178/78‑1 (MQ=255)
                                                                                      acaTACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAg                                                                                                                                >  7:124178/1‑78 (MQ=255)
                                                                                            ttCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTTACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCg                                                     >  1:93056/1‑147 (MQ=255)
                                                                                            ttCACCTCGATGTCACGCTCACAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACACTTGCCGTAGCGCCTCATGAACGACAACCCGGGTACGCCg                                                     >  4:100685/1‑147 (MQ=255)
                                                                                              cACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAgg                                                 >  1:92833/1‑149 (MQ=255)
                                                                                               aCCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAgg                                                 >  6:290296/1‑148 (MQ=255)
                                                                                                 cTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCggg                                                            >  5:362073/1‑135 (MQ=255)
                                                                                                 cTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCggg                                                            <  6:362073/135‑1 (MQ=255)
                                                                                                 cTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAg                                              >  7:128651/1‑149 (MQ=255)
                                                                                                  tCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCa                                               <  7:141342/147‑1 (MQ=255)
                                                                                                  tCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCa                                               >  8:141342/1‑147 (MQ=255)
                                                                                                     aTGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCAt                                          <  8:128651/149‑1 (MQ=255)
                                                                                                                  aGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATa                              >  4:48942/1‑148 (MQ=255)
                                                                                                                  aGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATa                              <  3:48942/148‑1 (MQ=255)
                                                                                                                   gCGTTTCTGGGCGGTCAGCGGTTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGTGTACGCCGTAGGCAGCCATGTTCAGGTCATAAc                            <  1:49475/149‑1 (MQ=255)
                                                                                                                      tttCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATAACgca                         <  1:360430/149‑1 (MQ=255)
                                                                                                                               ggttAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATAACGCAGCATCCAc                 >  7:187283/1‑148 (MQ=255)
                                                                                                                               ggtcAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTAccc                                                                                            >  6:285498/1‑73 (MQ=255)
                                                                                                                               ggtcAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTAccc                                                                                            <  5:285498/73‑1 (MQ=255)
                                                                                                                                     cGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATAACGCAGCATCCACGAACGCt          >  4:205098/1‑149 (MQ=255)
                                                                                                                                         ttGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATCACGCAGCATCCACGAACGCTCCtt      >  2:52700/1‑149 (MQ=255)
                                                                                                                                             ccGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATAACGCAGCATCCACGAACGCTCCTTGGgc  >  5:285352/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGTCCAGATCCGACTCGAACGCCGTGGCCCGGGTGGTGGCCTGGCGAACCCGCAACGACAGGTCCTTGGCCGCGCCCGTCTGCACCACATACTTCACCTCGATGTCACGCTCCCAGCGTTTCTGGTCGGTCAGCGGGTTGCCCGCAACGTCACGGCGCATGTAGGTGGCGTTGGCATTGGAGTAGTCAGCATCGTTACCCTTGCCGTAGCGCGTCATGAACGACAACCCGGGTACGCCGTAGGCAGCCATGTTCAGGTCATAACGCAGCATCCACGAACGCTCCTTGGGC  >  NC_002947/4126058‑4126347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑