Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,246,188	C→G	10.2%	S29S (TCG→TCC)	alr ←	alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,246,188	0	C	G	10.2%	147.8 / 6.7	57	S29S (TCG→TCC)	alr	alanine racemase
Reads supporting (aligned to +/- strand):  ref base C (18/32);  new base G (2/4);  total (21/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

AGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTT  >  NC_002947/4246040‑4246334                                                                                                                                                     |                                                                                                                                                      aGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGTTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATc                                                                                                                                                       >  6:184911/1‑149 (MQ=255)   gCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGa                                                                                                                                                     >  8:147847/1‑149 (MQ=255)   gCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGa                                                                                                                                                     >  8:143115/1‑149 (MQ=255)         gCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGTCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATGGACAA‑Cgg                                                                                                                                              <  1:171240/149‑1 (MQ=255)           cTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATGGACAA‑Cggca                                                                                                                                            <  1:139705/149‑3 (MQ=255)           cTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTACCGTTGTCGATGGACAA‑Cggca                                                                                                                                            <  3:76557/149‑3 (MQ=255)            ttGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑Cggtgg                                                                                                                                           >  2:38363/1‑149 (MQ=255)               aGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGc                                                                                                                                        <  8:150757/149‑1 (MQ=255)               aGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGc                                                                                                                                        <  5:20480/149‑1 (MQ=255)                gCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGcc                                                                                                                                       <  7:41475/149‑1 (MQ=255)                 cACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTTCAGGGCGCTGGCGCTGACTTAGCCCCAAGCATTGCAGTTTTTGACCGGCAGGGTGTTGGTTCCCTTGTCCATAGACAA‑CGGTTGTGCCg                                                                                                                                      >  1:98283/1‑149 (MQ=255)                  aCGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGc                                                                                                                                     <  7:19512/149‑1 (MQ=255)                           aGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑gggg                                                                                                                           >  1:342601/1‑149 (MQ=255)                             cTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑gggg‑gg                                                                                                                        <  3:92400/149‑1 (MQ=255)                                ggACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGTTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGc                                                                                                                       <  5:184911/147‑1 (MQ=255)                                      gCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCg                                                                                                                <  5:117094/148‑1 (MQ=255)                                      gCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGCGGTGCCGCATACA‑GGGG‑GGCCTGTcc                                                                                                                 <  8:21059/147‑1 (MQ=255)                                       ccGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACCGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCg                                                                                                                <  1:38363/148‑1 (MQ=255)                                         ggCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGa                                                                                                            >  8:259101/1‑149 (MQ=255)                                               cTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGaa                                                                                                      <  3:6371/149‑1 (MQ=255)                                                  ggCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAg                                                                                                     <  2:98283/147‑1 (MQ=255)                                                        cAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATCGACAA‑CGGTGGTGcc                                                                                                                                       <  4:230288/109‑1 (MQ=255)                                                        cAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGcc                                                                                                                                       >  3:230288/1‑109 (MQ=255)                                                        cAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAg                                                                                               <  5:355803/147‑1 (MQ=255)                                                               cGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGc                                                                                      <  7:259101/149‑1 (MQ=255)                                                                       gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAggg                                                                              >  3:165754/1‑149 (MQ=255)                                                                       gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAgg                                                                               <  7:143115/148‑1 (MQ=255)                                                                       gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAgg                                                                               <  7:271403/148‑1 (MQ=255)                                                                       gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAgg                                                                               <  7:147847/148‑1 (MQ=255)                                                                          ctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTAc                                                                           >  2:58145/1‑149 (MQ=255)                                                                           tgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGt                                                                                                    >  2:39866/1‑123 (MQ=255)                                                                           tgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGt                                                                                                    <  1:39866/123‑1 (MQ=255)                                                                                   gctggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGGCGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAg                                                                   <  1:98968/149‑1 (MQ=255)                                                                                     tggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCAGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCAAGGGATGCAGCCAGAAGGGTACGTCGAAAtgg                                                                 >  4:272116/1‑148 (MQ=255)                                                                                       gcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGGCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCAt                                                              <  1:58145/149‑1 (MQ=255)                                                                                             aCTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAt                                                        <  2:342601/149‑1 (MQ=255)                                                                                                  gACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTTGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                   <  7:199857/149‑1 (MQ=255)                                                                                                  gACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                   <  4:165754/149‑1 (MQ=255)                                                                                                       aGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtgt                                               >  7:195160/1‑148 (MQ=255)                                                                                                              gCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                   >  7:121358/1‑137 (MQ=255)                                                                                                              gCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                   <  8:121358/137‑1 (MQ=255)                                                                                                              gCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCg                                       >  1:183941/1‑149 (MQ=255)                                                                                                                tGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTAc                                                                           >  8:227126/1‑111 (MQ=255)                                                                                                                tGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTAc                                                                           <  7:227126/111‑1 (MQ=255)                                                                                                                       cACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTgcgc                               <  2:183941/148‑1 (MQ=255)                                                                                                                          ggTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCACAAGGGTACGGCGATAGGGCATGGTGATTACTCGTGTTCTTTTCGTAGTGCGCGCCt                           >  7:281243/1‑149 (MQ=255)                                                                                                                            tCAGGGTGTTGGTGCCGTTGTACATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGCTCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTa                         <  8:283822/149‑1 (MQ=255)                                                                                                                              aGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTTCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAAc                       <  3:272116/149‑1 (MQ=255)                                                                                                                               gggTGGTGGTGCCGTTGTCGATCGTCAA‑CGGTGGTGCCGCATACA‑GGGG‑GGGCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGTGCATTGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAAc                       <  8:281243/148‑1 (MQ=255)                                                                                                                                       gTGCCTGTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGGCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg               <  8:195160/148‑1 (MQ=255)                                                                                                                                         gCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgt             <  6:280961/148‑1 (MQ=255)                                                                                                                                         gCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgt             >  5:280961/1‑148 (MQ=255)                                                                                                                                         accGTTGTCGATGGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTc                                        <  4:228904/120‑1 (MQ=255)                                                                                                                                         accGTTGTCGATGGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTc                                        >  3:228904/2‑121 (MQ=255)                                                                                                                                          ccGTTGTCGATGGACAA‑CCGTGGTGCAGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGCATGCAGCAAGAAGGGTACGGCGAAAGGGCATGGTGATTAGTCGTGTTCTTGTCGAAGTGCGCTCATTAACAGCCGCATgtgt           >  4:190968/1‑149 (MQ=255)                                                                                                                                                   aTCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCGAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTc                                        <  8:332216/111‑1 (MQ=255)                                                                                                                                                   aTCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTc                                        >  7:332216/1‑111 (MQ=255)                                                                                                                                                    tCGACAA‑CGGTGGTGCCGCATACAGGGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  1:27344/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      AGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAA‑CGGTGGTGCCGCATACA‑GGGG‑GGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTT  >  NC_002947/4246040‑4246334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑