Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,357,496	G→C	7.2%	V289L (GTG→CTG) ‡	PP_3831 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,357,496	0	G	C	7.2%	211.5 / 4.4	69	V289L (GTG→CTG) ‡	PP_3831	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/34);  new base C (3/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCAAGGACTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCAGGCACTTGA  >  NC_002947/4357351‑4357635                                                                                                                                                   |                                                                                                                                            tGCCAAGGACTATCGCACCTGGGCCGCCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAgtgt                                                                                                                                          >  6:193197/1‑149 (MQ=255)   ccAAGGACTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGc                                                                                                                                        >  3:68194/1‑149 (MQ=255)       ggACTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGgc                                                                                                                                    >  4:245776/1‑149 (MQ=255)         aCTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGgcgc                                                                                                                                  <  1:326201/149‑1 (MQ=255)         aCTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGgcg                                                                                                                                   <  2:288658/148‑1 (MQ=255)         aCTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGgcg                                                                                                                                   >  1:288658/1‑148 (MQ=255)          cTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCgct                                                                                                                                 <  3:245776/149‑1 (MQ=255)           tATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCgctg                                                                                                                                >  6:267606/1‑149 (MQ=255)             tCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCgctgct                                                                                                                              <  5:334278/149‑1 (MQ=255)                cACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACa                                                                                                                           <  5:193197/149‑1 (MQ=255)                cACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACa                                                                                                                           <  2:301122/149‑1 (MQ=255)                 aCCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACAt                                                                                                                          >  4:27493/1‑149 (MQ=255)                 aCCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCACACTGCCGgcgta                                                                                                                                 <  8:201712/142‑3 (MQ=255)                 aCCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCACACTGCCGgcgta                                                                                                                                 >  7:201712/1‑140 (MQ=255)                        ccGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGgcgc                                                                                                                                  >  2:47131/1‑134 (MQ=255)                        ccGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGgcgc                                                                                                                                  <  1:47131/134‑1 (MQ=255)                         cGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCgg                                                                                                                  >  2:10814/1‑149 (MQ=255)                           gcagcaGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGAGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCAACATCAACCCgg                                                                                                                  >  1:301643/1‑147 (MQ=255)                                   cTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAATGTGCCGGCGCTGCTACATCCACCCGCCAGTTCTGGa                                                                                                        >  4:310246/1‑149 (MQ=255)                                    tGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑ACGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCCCTGCTACATCCACCCCGCAGTTCTGGAg                                                                                                       >  7:192236/1‑149 (MQ=255)                                        aTTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCAc                                                                                                    >  5:370424/1‑148 (MQ=255)                                         ttGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTa                                                                                                  >  6:340760/1‑149 (MQ=255)                                            aaCCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATg                                                                                                <  1:10814/148‑1 (MQ=255)                                            aaCCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGc                                                                                               >  8:229583/1‑149 (MQ=255)                                               cTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCtt                                                                                            >  2:111263/1‑149 (MQ=255)                                                tGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTATGGAGCACTATGCCTTg                                                                                           >  6:253354/1‑149 (MQ=255)                                                  cTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTggg                                                                                         <  5:340760/149‑1 (MQ=255)                                                  cTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTgg                                                                                          <  3:27493/148‑1 (MQ=255)                                                     aaGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGgc                                                                                       <  2:283612/148‑1 (MQ=255)                                                      aGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGgcgc                                                                                     >  6:164656/1‑149 (MQ=255)                                                       gCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGcc                                                                                    <  4:260169/149‑1 (MQ=255)                                                          cTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTggg                                                                                         >  1:10139/1‑141 (MQ=255)                                                          cTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTggg                                                                                         <  2:10139/141‑1 (MQ=255)                                                          cTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTgg                                                                                 <  7:85621/149‑1 (MQ=255)                                                          cTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTgg                                                                                 <  1:332019/149‑1 (MQ=255)                                                           ttGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGTGCCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTgg                                                                                 >  1:269894/1‑149 (MQ=255)                                                             ggCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGcc                                                                               <  5:164656/148‑1 (MQ=255)                                                                   ggAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAAttt                                                                          <  7:229583/147‑1 (MQ=255)                                                                      gCCGTAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGG‑CCGCCAGGTAGCCACGCGCCTGGGCAATTCGCCGGCAGTTTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCATTGGGGCGCCTGGCCAATTTGCCg                                                                      <  8:192236/148‑1 (MQ=255)                                                                              gTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCgt                                                             <  4:68194/149‑1 (MQ=255)                                                                               tGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCgtg                                                            >  5:191302/1‑149 (MQ=255)                                                                                 aaGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCgtgt                                                           <  4:24180/148‑1 (MQ=255)                                                                                 aaGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCgtgt                                                           >  3:24180/1‑148 (MQ=255)                                                                                             aGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTg                                              <  2:269894/149‑1 (MQ=255)                                                                                             aGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGACTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTg                                              >  4:103930/1‑149 (MQ=255)                                                                                                  gccgcAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGCGCGCAAAGGCCTGGAccc                                         >  5:6999/1‑149 (MQ=255)                                                                                                     gcAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCgga                                      <  3:137522/149‑1 (MQ=255)                                                                                                     gcAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCgga                                      <  3:132183/149‑1 (MQ=255)                                                                                                            t‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGt                                <  1:89981/148‑1 (MQ=255)                                                                                                            t‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGt                                >  2:89981/1‑148 (MQ=255)                                                                                                            t‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTg                               >  8:117298/1‑149 (MQ=255)                                                                                                               cGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGaa                                  <  1:60280/144‑1 (MQ=255)                                                                                                               cGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGaa                                  >  2:60280/1‑144 (MQ=255)                                                                                                               cGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTgg                              <  6:191302/148‑1 (MQ=255)                                                                                                                        gCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCtgtt                    <  5:253354/149‑1 (MQ=255)                                                                                                                         ccACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCtgttg                   >  3:141322/1‑149 (MQ=255)                                                                                                                          cACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTg                                              <  4:67184/121‑1 (MQ=255)                                                                                                                          cACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTg                                              >  3:67184/1‑121 (MQ=255)                                                                                                                           aCGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTg                                              <  6:97375/120‑1 (MQ=255)                                                                                                                           aCGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTg                                              >  5:97375/1‑120 (MQ=255)                                                                                                                           aCGCGCCGGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTgttgtt                 <  5:123949/149‑1 (MQ=255)                                                                                                                               gcCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTttctt             >  5:57776/1‑149 (MQ=255)                                                                                                                                 cTGGGCATTACGCCGGCAGTGTGGCGGCGCTGCTTCATCCACCCGGCAGTTCTGTAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAATTACCGTGTGCGCAAAGGGCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCa           <  3:103930/149‑1 (MQ=255)                                                                                                                                    ggCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCa           <  6:117049/146‑1 (MQ=255)                                                                                                                                    ggCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCa           >  5:117049/1‑146 (MQ=255)                                                                                                                                    ggCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCAg          <  6:370424/147‑1 (MQ=255)                                                                                                                                         agcGCCGGCACACTGCCGGCGCTGCTACATCCACGCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCAGGCACtt    >  8:59705/3‑148 (MQ=255)                                                                                                                                         agcGCCGGCACACTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCa           >  3:168732/3‑141 (MQ=255)                                                                                                                                         agcGCCGGCACACTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCa           <  4:168732/139‑1 (MQ=255)                                                                                                                                           cggcGGGAGTGTGGCGGGGCTGCTTGATCCTCGCGGGAGTTCTGGAGGTCTATGCCTTGGGGCGCCTGGCCTATTTGGCGTTAAAGGCTGTGCGCATAGGCCTGGTCGCGGTGGATGTGGGCTTGGTGTTGTTTCTTCAGGCAGTTGa  <  2:301643/145‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            TGCCAAGGACTATCGCACCTGGGCCGGCAGCAGCCTGGCATTGAACCTGCTAAAGCCCTTGGCCTGGGAGCCGGAGAGTGAAGCCAAACGCCAGGTCGCCGCAATAGT‑CCGCCAGGTAGCCACGCGCCTGGGCAATACGCCGGCAGTGTGCCGGCGCTGCTACATCCACCCGGCAGTTCTGGAGCACTATGCCTTGGGGCGCCTGGCCAATTTGCCGAAAAACCGTGTGCGCAAAGGCCTGGACCCGGAGGAAGTGGCCTTGCTGTTGTTTCTTCAGGCACTTGA  >  NC_002947/4357351‑4357635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑