Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,425,139	G→C	10.9%	intergenic (‑197/‑603)	PP_5647 ← / → PP_3917	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,425,139	0	G	C	10.9%	183.7 / 11.5	64	intergenic (‑197/‑603)	PP_5647/PP_3917	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (3/4);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

ATCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGC  >  NC_002947/4424994‑4425280                                                                                                                                                  |                                                                                                                                              atCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCgg                                                                                                                                            >  4:19377/1‑149 (MQ=255)     aTTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggct                                                                                                                                        >  7:267238/1‑149 (MQ=255)      tttCGCTTAAATGCGACCGGTGGGAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctg                                                                                                                                       <  1:213805/149‑1 (MQ=255)        tCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGTGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                      <  2:213274/148‑1 (MQ=255)          gCTTAAATGCGACCGGTGGAAAATGATCTGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCCCCgcgctgggct                                                                                                                                   >  8:230502/1‑149 (MQ=255)           cTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                  <  7:221181/149‑1 (MQ=255)              aaaaGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                <  3:282684/148‑1 (MQ=255)              aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTTATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGCTCACTGAGCCCGTCCAGCCGACAGAAGTTCAAGTAGCAAACAGCCCAGCCCCGCGCTGGGCtttt                                                                                                                                <  5:358094/148‑3 (MQ=255)              aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCCCCGCGCTGGGCtttt                                                                                                                                <  2:345675/148‑3 (MQ=255)               aaagCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCACCGCGGGGCTGGGCTTCTGt                                                                                                                              <  3:66395/146‑1 (MQ=255)                 tGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                     >  4:83170/1‑140 (MQ=255)                 tGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                     <  3:83170/140‑1 (MQ=255)                   cGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcag                                                                                                                           <  8:267238/148‑1 (MQ=255)                         gTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCt                                                                                                                    >  3:134065/1‑149 (MQ=255)                         gTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAACAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCt                                                                                                                    >  3:299665/1‑149 (MQ=255)                          tGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTc                                                                                                                   <  2:74748/149‑1 (MQ=255)                            gAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                      <  2:281691/128‑1 (MQ=255)                            gAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                      >  1:281691/1‑128 (MQ=255)                              aaaTGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCATGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACt                                                                                                               <  7:288853/149‑1 (MQ=255)                                    tCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACt                                                                                                          >  3:161969/1‑148 (MQ=255)                                         ccTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGt                                                                                                    >  1:34120/1‑149 (MQ=255)                                            aaTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            <  2:237760/138‑1 (MQ=255)                                            aaTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            >  1:237760/1‑138 (MQ=255)                                             aTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTc                                                                                                  <  4:134065/147‑1 (MQ=255)                                                cTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAAtt                                                                                              <  7:59540/148‑1 (MQ=255)                                                 ttGCTGATATATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGt                                                                                                    <  8:69475/141‑1 (MQ=255)                                                 ttGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGt                                                                                                    >  7:69475/1‑141 (MQ=255)                                                   gCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACg                                                                                           <  2:223375/148‑1 (MQ=255)                                                     tGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCtt                                                                                        >  1:216256/1‑149 (MQ=255)                                                         agataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            >  6:102318/1‑125 (MQ=255)                                                         agataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            <  5:102318/125‑1 (MQ=255)                                                          gataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTAc                                                                                            <  4:214656/140‑1 (MQ=255)                                                          gataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTAc                                                                                            >  3:214656/1‑140 (MQ=255)                                                           atatCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGc                                                                                  >  3:124067/1‑149 (MQ=255)                                                             atCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                      >  6:78109/1‑143 (MQ=255)                                                             atCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                      <  5:78109/143‑1 (MQ=255)                                                             atCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTa                                                                                >  8:310233/1‑149 (MQ=255)                                                                      aCAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCa                                                                                                        <  2:281909/116‑1 (MQ=255)                                                                      aCAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCa                                                                                                        >  1:281909/1‑116 (MQ=255)                                                                        aGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGgc                                                                     <  7:310233/149‑1 (MQ=255)                                                                                   agaACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAg                                                          <  4:124067/149‑1 (MQ=255)                                                                                     aaCCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTAGGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGc                                                         >  5:347769/1‑148 (MQ=255)                                                                                     aaCCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGc                                                         >  7:288249/1‑148 (MQ=255)                                                                                        cGAGCCCGATCACTGAGCCCGTCCAACAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCtata                                                     <  4:299665/149‑1 (MQ=255)                                                                                             ccGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCt                                                <  2:35942/149‑1 (MQ=255)                                                                                               gATCACTGAGCCGGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCg                                              >  6:371656/1‑149 (MQ=255)                                                                                               gATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCg                                              >  6:163337/1‑149 (MQ=255)                                                                                                   aCTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCAt                                          <  3:19377/149‑1 (MQ=255)                                                                                                            gTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGccc                                 <  2:34120/149‑1 (MQ=255)                                                                                                                   agacagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATgg                          >  2:11318/1‑149 (MQ=255)                                                                                                                   agacagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATgg                          <  5:371656/149‑1 (MQ=255)                                                                                                                     acagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGt                        >  2:38043/1‑149 (MQ=255)                                                                                                                           gTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                            >  6:282646/1‑139 (MQ=255)                                                                                                                           gTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                            <  5:282646/139‑1 (MQ=255)                                                                                                                                aGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                             <  7:199604/85‑1 (MQ=255)                                                                                                                                aGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                             >  8:199604/1‑85 (MQ=255)                                                                                                                                    gCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGtt         >  7:59202/1‑149 (MQ=255)                                                                                                                                     acagaaGCCCAGCCCCGCGCTGGGCTTTTGTCAGCAGAGCTCGACTAGACTCAGCGTTCATTTACGCTTGGCAGCTACTCCAATGGgcg                                                                    <  1:201406/85‑1 (MQ=37)                                                                                                                                     acagaaGCCCAGCCCCGCGCTGGGCTTTTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGgcg                                                                    >  2:201406/5‑89 (MQ=37)                                                                                                                                     acagaaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTg                                        >  1:311315/5‑117 (MQ=255)                                                                                                                                     acagaaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTg                                        <  2:311315/113‑1 (MQ=255)                                                                                                                                       aaaaGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCGTCGATGGGTCCGGTGGGCCTTGTTTCt      <  2:216256/149‑1 (MQ=255)                                                                                                                                        aaaGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTg     >  5:180612/1‑149 (MQ=255)                                                                                                                                           gCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGCCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTgcgc  >  7:314991/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                              ATCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGC  >  NC_002947/4424994‑4425280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑