Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,425,143 G→C 10.7% intergenic (‑201/‑599) PP_5647 ← / → PP_3917 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,425,1430GC10.7% 160.9 / 7.4 55intergenic (‑201/‑599)PP_5647/PP_3917hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base C (2/4);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

ATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGC  >  NC_002947/4424998‑4425280
                                                                                                                                                 |                                                                                                                                         
aTTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggct                                                                                                                                        >  7:267238/1‑149 (MQ=255)
 tttCGCTTAAATGCGACCGGTGGGAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctg                                                                                                                                       <  1:213805/149‑1 (MQ=255)
   tCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGTGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                      <  2:213274/148‑1 (MQ=255)
     gCTTAAATGCGACCGGTGGAAAATGATCTGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCCCCgcgctgggct                                                                                                                                   >  8:230502/1‑149 (MQ=255)
      cTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                  <  7:221181/149‑1 (MQ=255)
         aaaaGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                <  3:282684/148‑1 (MQ=255)
         aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTTATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGCTCACTGAGCCCGTCCAGCCGACAGAAGTTCAAGTAGCAAACAGCCCAGCCCCGCGCTGGGCtttt                                                                                                                                <  5:358094/148‑3 (MQ=255)
         aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCCCCGCGCTGGGCtttt                                                                                                                                <  2:345675/148‑3 (MQ=255)
          aaagCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCACCGCGGGGCTGGGCTTCTGt                                                                                                                              <  3:66395/146‑1 (MQ=255)
            tGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                     >  4:83170/1‑140 (MQ=255)
            tGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                     <  3:83170/140‑1 (MQ=255)
              cGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcag                                                                                                                           <  8:267238/148‑1 (MQ=255)
                    gTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCt                                                                                                                    >  3:134065/1‑149 (MQ=255)
                    gTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAACAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCt                                                                                                                    >  3:299665/1‑149 (MQ=255)
                     tGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTc                                                                                                                   <  2:74748/149‑1 (MQ=255)
                       gAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                      >  1:281691/1‑128 (MQ=255)
                       gAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                      <  2:281691/128‑1 (MQ=255)
                         aaaTGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCATGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACt                                                                                                               <  7:288853/149‑1 (MQ=255)
                               tCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACt                                                                                                          >  3:161969/1‑148 (MQ=255)
                                    ccTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGt                                                                                                    >  1:34120/1‑149 (MQ=255)
                                       aaTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            <  2:237760/138‑1 (MQ=255)
                                       aaTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            >  1:237760/1‑138 (MQ=255)
                                        aTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTc                                                                                                  <  4:134065/147‑1 (MQ=255)
                                           cTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAAtt                                                                                              <  7:59540/148‑1 (MQ=255)
                                            ttGCTGATATATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGt                                                                                                    <  8:69475/141‑1 (MQ=255)
                                            ttGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGt                                                                                                    >  7:69475/1‑141 (MQ=255)
                                              gCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACg                                                                                           <  2:223375/148‑1 (MQ=255)
                                                tGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCtt                                                                                        >  1:216256/1‑149 (MQ=255)
                                                    agataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            >  6:102318/1‑125 (MQ=255)
                                                    agataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                            <  5:102318/125‑1 (MQ=255)
                                                     gataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTAc                                                                                            >  3:214656/1‑140 (MQ=255)
                                                     gataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTAc                                                                                            <  4:214656/140‑1 (MQ=255)
                                                      atatCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGc                                                                                  >  3:124067/1‑149 (MQ=255)
                                                        atCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                      >  6:78109/1‑143 (MQ=255)
                                                        atCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                      <  5:78109/143‑1 (MQ=255)
                                                        atCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTa                                                                                >  8:310233/1‑149 (MQ=255)
                                                                 aCAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCa                                                                                                        <  2:281909/116‑1 (MQ=255)
                                                                 aCAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCa                                                                                                        >  1:281909/1‑116 (MQ=255)
                                                                   aGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGgc                                                                     <  7:310233/149‑1 (MQ=255)
                                                                              agaACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAg                                                          <  4:124067/149‑1 (MQ=255)
                                                                                aaCCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTAGGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGc                                                         >  5:347769/1‑148 (MQ=255)
                                                                                aaCCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGc                                                         >  7:288249/1‑148 (MQ=255)
                                                                                   cGAGCCCGATCACTGAGCCCGTCCAACAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCtata                                                     <  4:299665/149‑1 (MQ=255)
                                                                                        ccGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCt                                                <  2:35942/149‑1 (MQ=255)
                                                                                          gATCACTGAGCCGGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCg                                              >  6:371656/1‑149 (MQ=255)
                                                                                          gATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCg                                              >  6:163337/1‑149 (MQ=255)
                                                                                              aCTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCAt                                          <  3:19377/149‑1 (MQ=255)
                                                                                                       gTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGccc                                 <  2:34120/149‑1 (MQ=255)
                                                                                                              agacagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATgg                          <  5:371656/149‑1 (MQ=255)
                                                                                                              agacagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATgg                          >  2:11318/1‑149 (MQ=255)
                                                                                                                acagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGt                        >  2:38043/1‑149 (MQ=255)
                                                                                                                      gTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                            >  6:282646/1‑139 (MQ=255)
                                                                                                                      gTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                            <  5:282646/139‑1 (MQ=255)
                                                                                                                           aGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                             <  7:199604/85‑1 (MQ=255)
                                                                                                                           aGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                             >  8:199604/1‑85 (MQ=255)
                                                                                                                               gCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGtt         >  7:59202/1‑149 (MQ=255)
                                                                                                                                acagaaGCCCAGCCCCGCGCTGGGCTTTTGTCAGCAGAGCTCGACTAGACTCAGCGTTCATTTACGCTTGGCAGCTACTCCAATGGgcg                                                                    <  1:201406/85‑1 (MQ=37)
                                                                                                                                acagaaGCCCAGCCCCGCGCTGGGCTTTTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGgcg                                                                    >  2:201406/5‑89 (MQ=37)
                                                                                                                                acagaaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTg                                        >  1:311315/5‑117 (MQ=255)
                                                                                                                                acagaaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTg                                        <  2:311315/113‑1 (MQ=255)
                                                                                                                                  aaaaGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCGTCGATGGGTCCGGTGGGCCTTGTTTCt      <  2:216256/149‑1 (MQ=255)
                                                                                                                                   aaaGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTg     >  5:180612/1‑149 (MQ=255)
                                                                                                                                      gCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGCCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTgcgc  >  7:314991/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
ATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGC  >  NC_002947/4424998‑4425280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑