Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,458,747 A→T 7.2% intergenic (+39/‑229) pcaJ → / → trkH‑I 3‑oxoadipate CoA‑transferase subunit A/potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,458,7470AT 7.2% 209.1 / 4.4 69intergenic (+39/‑229)pcaJ/trkH‑I3‑oxoadipate CoA‑transferase subunit A/potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base T (3/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG  >  NC_002947/4458602‑4458895
                                                                                                                                                 |                                                                                                                                                    
tGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaat                                                                                                                                                    >  3:298828/1‑148 (MQ=255)
 ggCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatc                                                                                                                                                   >  3:58017/1‑148 (MQ=255)
  gCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatc                                                                                                                                                   <  8:273220/147‑1 (MQ=255)
   ccGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaat                                                                                                                                                <  4:58017/149‑1 (MQ=255)
        cTGGAAGTGACACCTGCAGGGCTGAAAGGGGGCGAAATCGGCGCGGACATCGACTTTGACGACCTGCAGAAACTCAGGGGCGTGCCGCGGATCAAGTGATCCAGCCAGACTTAGGGCTTTCCATGCCTCTCCGGCACAATCAATAGTg                                                                                                                                            <  8:305654/148‑1 (MQ=255)
               tGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGcac                                                                                                                                      >  5:6213/1‑147 (MQ=255)
                  cacCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAAATCACTGGCGAGCCGCTGATCAAGTCAACCACGAAGACTTAGGGCTTTCCATGACTCTGCGTCAAAATCAATAGTGCCGCACAAccg                                                                                                                                 >  6:102136/1‑149 (MQ=255)
                   acCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAgcgc                                                                                                                                >  5:122751/1‑149 (MQ=255)
                     cTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccg                                                                                                                              >  3:100797/1‑149 (MQ=255)
                       gAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGATTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc                                                                                                                             >  2:83190/1‑148 (MQ=255)
                           ggCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCa                                                                                                                        <  6:6213/149‑1 (MQ=255)
                                ataGTGGGCGAAATCTGCGCGGACTTCGTCTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTTATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGa                                                                                                                    <  5:102136/146‑1 (MQ=255)
                                       tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCgg                                                                                                            <  6:122751/149‑1 (MQ=255)
                                         gAAATCTGCGCGGACATCGACTTTGACGAGCTGCCGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTg                                                                                                          <  2:258887/149‑1 (MQ=255)
                                          aaaTCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGc                                                                                                         <  4:100797/149‑1 (MQ=255)
                                            aTCTGCGCTGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCtt                                                                                                       <  7:135771/149‑1 (MQ=255)
                                            aTCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCtt                                                                                                       <  4:147783/149‑1 (MQ=255)
                                            aTCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCtt                                                                                                       <  2:230172/149‑1 (MQ=255)
                                                 cgcgGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTg                                                                                                      <  2:103216/145‑1 (MQ=255)
                                                 cgcgGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTg                                                                                                      <  5:53142/145‑1 (MQ=255)
                                                 cgcgGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTg                                                                                                      <  6:3484/145‑1 (MQ=255)
                                                    ggACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTAc                                                                                               <  1:83190/149‑1 (MQ=255)
                                                    ggACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTAc                                                                                               <  5:136326/149‑1 (MQ=255)
                                                        aTCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATa                                                                                                                                               >  8:62613/1‑97 (MQ=255)
                                                        aTCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATa                                                                                                                                               <  7:62613/97‑1 (MQ=255)
                                                        aTCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCttt                                                                                           <  4:60336/149‑1 (MQ=255)
                                                        aTCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCtt                                                                                            >  3:60336/1‑148 (MQ=255)
                                                          agACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGACTCTGCGACACAATCAATAGTGCCGCACAAGCGCCGCGAGAAGGGAGCACGCGGTGCTTGAGACTACCTTTa                                                                                          >  7:233149/2‑148 (MQ=255)
                                                                 gACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGc                                                                                   <  2:50709/148‑1 (MQ=255)
                                                                      gCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGTTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGaa                                                                             <  2:190919/149‑1 (MQ=255)
                                                                      gCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCg                                                                               >  8:155438/1‑147 (MQ=255)
                                                                        tGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGc                                                                           >  2:150911/1‑149 (MQ=255)
                                                                         gCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGc                                                                                      <  7:278724/137‑1 (MQ=255)
                                                                         gCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGc                                                                                      >  8:278724/1‑137 (MQ=255)
                                                                         gCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGcc                                                                          >  8:217064/1‑149 (MQ=255)
                                                                         gCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGcc                                                                          >  1:297768/1‑149 (MQ=255)
                                                                          cAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGcc                                                                          >  2:39302/1‑148 (MQ=255)
                                                                          cAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGcc                                                                          >  5:329067/1‑148 (MQ=255)
                                                                          cAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGcc                                                                          >  1:310949/1‑148 (MQ=255)
                                                                           aGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCt                                                                                             <  2:326575/128‑1 (MQ=255)
                                                                           aGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCt                                                                                             >  1:326575/1‑128 (MQ=255)
                                                                            cttgatCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGAt                                                                       >  6:56095/6‑149 (MQ=255)
                                                                               aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATgg                                                                     >  2:82042/1‑148 (MQ=255)
                                                                                   aGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAActg                                                                >  8:91110/1‑148 (MQ=255)
                                                                                         gTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGgag                                                          <  4:298828/149‑1 (MQ=255)
                                                                                            ccGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGagag                                                        <  2:310949/148‑1 (MQ=255)
                                                                                            ccGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAgg                                                       <  1:82042/149‑1 (MQ=255)
                                                                                              gCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGaa                                                                             >  4:165834/1‑125 (MQ=255)
                                                                                              gCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGaa                                                                             <  3:165834/125‑1 (MQ=255)
                                                                                               cTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTc                                                     >  2:309788/1‑148 (MQ=255)
                                                                                                   tCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAAcc                                                 <  1:309788/148‑1 (MQ=255)
                                                                                                     aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCaa                                              >  7:278237/1‑149 (MQ=255)
                                                                                                      aGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAAt                                             <  5:56095/149‑1 (MQ=255)
                                                                                                       gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATg                                            <  1:39302/149‑1 (MQ=255)
                                                                                                          aTCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGc                                         >  7:238569/1‑149 (MQ=255)
                                                                                                              aGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATc                                     >  7:143652/1‑149 (MQ=255)
                                                                                                               ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATc                                     >  3:142228/1‑148 (MQ=255)
                                                                                                                      ttAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCgg                               >  5:99321/1‑147 (MQ=255)
                                                                                                                      ttAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCgg                               >  3:207536/1‑147 (MQ=255)
                                                                                                                            cattCCATGCCTCTGCTGAACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa                       <  8:143652/147‑1 (MQ=255)
                                                                                                                            cTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATgg                                                                     >  2:338113/1‑103 (MQ=255)
                                                                                                                            cTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATgg                                                                     <  1:338113/103‑1 (MQ=255)
                                                                                                                                ccATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAg                    <  4:207536/148‑1 (MQ=255)
                                                                                                                                 cATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAg                                                                                                                       >  4:169760/1‑48 (MQ=255)
                                                                                                                                 cATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAg                                                                                                                       <  3:169760/48‑1 (MQ=255)
                                                                                                                                  aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcgg                 >  6:1552/1‑149 (MQ=255)
                                                                                                                                     ccTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGATCTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggcg               >  1:293954/1‑148 (MQ=255)
                                                                                                                                      ttgtGCGGCACTATTGATTGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGt              >  6:205322/4‑148 (MQ=255)
                                                                                                                                          gCGGCACTATTGATTGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa                                           >  4:42703/1‑115 (MQ=255)
                                                                                                                                          gCGGCACTATTGATTGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa                                           <  3:42703/115‑1 (MQ=255)
                                                                                                                                           cGGCACTATTGATTGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGa                             >  2:251721/1‑128 (MQ=255)
                                                                                                                                           cGGCACTATTGATTGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGa                             <  1:251721/128‑1 (MQ=255)
                                                                                                                                             gCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAAtt      <  1:150911/149‑1 (MQ=255)
                                                                                                                                               acaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGc    >  1:179231/1‑149 (MQ=255)
                                                                                                                                                caatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGcc   <  7:155438/149‑1 (MQ=255)
                                                                                                                                                 aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCg  <  2:297768/149‑1 (MQ=255)
                                                                                                                                                 aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCg  >  3:289239/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG  >  NC_002947/4458602‑4458895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑