Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,557,034 A→C 7.9% L148R (CTG→CGG)  gntZ ← 6‑phosphogluconate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,557,0340AC 7.9% 171.7 / 3.0 59L148R (CTG→CGG) gntZ6‑phosphogluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (25/29);  new base C (2/3);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTCG  >  NC_002947/4556886‑4557177
                                                                                                                                                    |                                                                                                                                               
gTACTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACa                                                                                                                                                 <  6:92806/149‑1 (MQ=255)
   cTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCg                                                                                                                                              <  6:315254/149‑1 (MQ=255)
        tGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTcc                                                                                                                                         <  2:116855/149‑1 (MQ=255)
          ccGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAg                                                                                                                                       <  2:233508/149‑1 (MQ=255)
             ttATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGgcg                                                                                                                                    <  8:59883/149‑1 (MQ=255)
             ttATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGgcg                                                                                                                                    >  7:334466/1‑149 (MQ=255)
                tGCACCATTTTCACGTTGTGCCCTGCGCCCGGTGGACCTGCATGGATGTAGCCGTGCTCGGCACGCTGGGGGTCGGCGCTGCGGCCATGGGGACGCGGGATTTCGCCGACGCGCGGCGCCAGCGGCTGGAACCGCTGTTCATGGCGCt                                                                                                                                  <  4:332438/148‑1 (MQ=255)
                tGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGGCGCtg                                                                                                                                 <  1:57043/149‑2 (MQ=255)
                   aCCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGaa                                                                                                                              >  7:260918/1‑149 (MQ=255)
                          tCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTcc                                                                                                                       >  3:207873/1‑149 (MQ=255)
                          tCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTc                                                                                                                        <  8:260918/148‑1 (MQ=255)
                              gTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCtttt                                                                                                                   <  5:290545/149‑1 (MQ=255)
                                    cccGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGgcgc                                                                                                                                   <  4:232521/127‑1 (MQ=255)
                                    cccGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGgcgc                                                                                                                                   >  3:232521/1‑127 (MQ=255)
                                    cccGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTcgccgc                                                                                                             >  6:171943/1‑149 (MQ=255)
                                    cccGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTcgccgc                                                                                                             >  1:126912/1‑149 (MQ=255)
                                     ccGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCgccgcc                                                                                                            <  2:294167/149‑1 (MQ=255)
                                                tGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGc                                                                                                 <  5:171943/149‑1 (MQ=255)
                                                              tGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTcc                                                                                   <  3:119269/149‑1 (MQ=255)
                                                               gtcgCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTCCGCGGGAGGTCGCCCCCGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCACGAACACGGCCGTTGCGCCGCCGAGCATCATGCAGTAGCCGCgggc                                                                                    >  6:228954/4‑145 (MQ=255)
                                                                tAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAg                                                                                 <  7:111987/149‑1 (MQ=255)
                                                                  cccGTGCTCGGCACGCTGGGGCTCGCCGCTGCGGCCATGGGGACCCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCGCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGc                                                                               <  8:161222/148‑1 (MQ=255)
                                                                   ccGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGAAGTAGCCGCGGTCCAGGcc                                                                              >  5:257384/1‑149 (MQ=255)
                                                                    cGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGccc                                                                             <  2:126912/149‑1 (MQ=255)
                                                                    cGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGccc                                                                             <  5:133260/149‑1 (MQ=255)
                                                                    cGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGccc                                                                             >  7:69503/1‑149 (MQ=255)
                                                                     gTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGACGCCCACGCCCGGCGCCAGCGCCGTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGccc                                                                             >  7:183027/1‑148 (MQ=255)
                                                                      tGCTCGGCACGCTGGTGCGCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCca                                                                           <  8:334466/149‑1 (MQ=255)
                                                                          cGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACAcg                                                                       >  1:114665/1‑149 (MQ=255)
                                                                               cGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACgccgcc                                                                  <  3:158739/149‑1 (MQ=255)
                                                                                       cTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGCACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTcc                                                          >  3:211068/1‑149 (MQ=255)
                                                                                        tCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTccc                                                         >  6:42741/1‑149 (MQ=255)
                                                                                          gccgcTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTccc                                                         <  5:83958/147‑1 (MQ=255)
                                                                                          gccgcTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCg                                                        <  2:114665/148‑1 (MQ=255)
                                                                                          gccgcTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGa                                                       >  1:301868/1‑149 (MQ=255)
                                                                                                  ggCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGTGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTc                                                   >  6:340952/1‑145 (MQ=255)
                                                                                                   gCCATGGGTACGCGGGTTGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGa                                               >  7:331075/1‑148 (MQ=255)
                                                                                                      aTGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAAt                                           >  4:337262/1‑149 (MQ=255)
                                                                                                       tGGGTACGGGGGTTGTCCCCCACGCCCGGCGCCAGCGCCTTGTACAGCGGATACATGCTCTCGAAAACGTGCTTTTCGCCTCCTATAATTATGCAGTAGCCGCTGTCCAGGCTCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATg                                          <  8:331075/149‑1 (MQ=255)
                                                                                                       tGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATg                                          <  5:42741/149‑1 (MQ=255)
                                                                                                           tACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGcc                                                                              <  1:205816/109‑1 (MQ=255)
                                                                                                           tACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGcc                                                                              >  2:205816/1‑109 (MQ=255)
                                                                                                              gcgGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCcg                                   >  7:307392/1‑149 (MQ=255)
                                                                                                                gggATGTCGCCCACGCCCGGCTCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGTGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCcgcg                                 <  5:340952/149‑1 (MQ=255)
                                                                                                                   aTGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCtt                              <  7:13698/149‑1 (MQ=255)
                                                                                                                      tCGCCCACGCCCGGCGCCAGCGCCGTGCACCGCGGTTCCAGGCGCTAGAACACGTCCTTTTCGCCGCCGATCCTCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGc                           <  8:183027/149‑1 (MQ=255)
                                                                                                                       cGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGcc                          <  3:229520/149‑1 (MQ=255)
                                                                                                                        gCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGc                           >  4:175890/1‑147 (MQ=255)
                                                                                                                              gCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTg                   <  6:257384/149‑1 (MQ=255)
                                                                                                                                  ggCGCCAGCGCCGTGACCAGCGGGTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACCCCGCCGGAAGTCCCGCCGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAgc               <  8:69503/149‑1 (MQ=255)
                                                                                                                                          cgcCTGGAACCGCTGTTCAAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCa       >  4:278662/1‑149 (MQ=255)
                                                                                                                                            ccTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCGGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATg     >  5:201221/1‑149 (MQ=255)
                                                                                                                                            ccTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATg     >  6:32545/1‑149 (MQ=255)
                                                                                                                                            ccTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATg     >  5:273994/1‑149 (MQ=255)
                                                                                                                                            ccTGGAACCGCTGTTCAAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATg     >  1:271426/1‑149 (MQ=255)
                                                                                                                                             cTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATgt    >  6:120178/1‑149 (MQ=255)
                                                                                                                                              ttGAACAGCGGTTCCAGGCGCTCTAACTCGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCTGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGa                                               <  2:252287/105‑1 (MQ=255)
                                                                                                                                              ttGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGTTCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGa                                               >  1:252287/1‑105 (MQ=255)
                                                                                                                                                gAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATgtcg  <  2:301868/148‑1 (MQ=255)
                                                                                                                                                gAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGCCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATgtcg  <  4:207873/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTACTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTCG  >  NC_002947/4556886‑4557177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑