Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,623,000 C→T 7.1% intergenic (+1593/+2971) PP_5654 → / ← PP_4091 phosphotriesterase/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,623,0000CT 7.1% 157.3 / 2.1 57intergenic (+1593/+2971)PP_5654/PP_4091phosphotriesterase/transposase
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

ACTCAACCACCTGTACTACGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCCGCGGGATGACCAAC  >  NC_002947/4622853‑4623144
                                                                                                                                                   |                                                                                                                                                
aCTCAACCACCTGTACTACGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTAGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCg                                                                                                                                                 >  1:208655/1‑149 (MQ=21)
 cTCAACCACCTGTACTACGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCgg                                                                                                                                                >  2:7123/1‑149 (MQ=34)
     accaccTGTACTACGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGc                                                                                                                                            <  7:108312/149‑1 (MQ=34)
        accTGTACTACGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTg                                                                                                                                         <  8:54070/149‑1 (MQ=37)
             tactacGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCg                                                                                                                                     >  3:9468/1‑148 (MQ=37)
                tacGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGa                                                                                                                                   >  7:140826/1‑147 (MQ=38)
                tacGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGa                                                                                                                                   >  4:210048/1‑147 (MQ=38)
                    gCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGc                                                                                                                              >  4:346049/1‑148 (MQ=39)
                      aGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTAt                                                                                                                           <  8:140826/149‑1 (MQ=39)
                      aGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTAt                                                                                                                           >  4:96982/1‑149 (MQ=39)
                        cGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTc                                                                                                                         <  4:171836/149‑1 (MQ=39)
                           gCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCg                                                                                                                                     >  7:238754/1‑134 (MQ=37)
                           gCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCg                                                                                                                                     <  8:238754/134‑1 (MQ=37)
                           gCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACt                                                                                                                      <  1:7123/149‑1 (MQ=39)
                             aCCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTAc                                                                                                                    >  6:286566/1‑149 (MQ=39)
                               cTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACcg                                                                                                                  <  3:96982/149‑1 (MQ=39)
                                   aCCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGATATTCACTACCGCGAt                                                                                                              >  8:73225/1‑149 (MQ=255)
                                     cAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGc                                                                                                            <  3:210048/149‑1 (MQ=39)
                                        aTCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGcccc                                                                                                         <  3:148613/149‑1 (MQ=39)
                                         tCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCct                                                                                                        <  4:21760/149‑1 (MQ=39)
                                           aaCCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCctgc                                                                                                      >  6:353208/1‑149 (MQ=39)
                                             ccTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTAtt                                                                                                                          >  6:352766/1‑127 (MQ=40)
                                             ccTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTAtt                                                                                                                          <  5:352766/127‑1 (MQ=40)
                                                    ggCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACCGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGtgct                                                                                             >  4:348386/1‑149 (MQ=255)
                                                     gCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTAcc                                                                                                                   >  4:180218/1‑126 (MQ=40)
                                                     gCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTAcc                                                                                                                   <  3:180218/126‑1 (MQ=40)
                                                                  cGATTTCGAATGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGAc                                                                                >  4:105836/1‑148 (MQ=255)
                                                                             gTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGc                                                                    >  6:366452/1‑149 (MQ=255)
                                                                                aTGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTa                                                                  <  1:121302/148‑1 (MQ=255)
                                                                                  gCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGCCACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGa                                                               >  5:286971/1‑149 (MQ=255)
                                                                                   cGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGAt                                                              <  4:9468/149‑1 (MQ=255)
                                                                                        cACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGa                                                               <  8:16481/143‑1 (MQ=255)
                                                                                        cACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGa                                                               >  7:16481/1‑143 (MQ=255)
                                                                                         acgacgAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGc                                                        <  5:286566/149‑1 (MQ=255)
                                                                                         acgacgAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGc                                                        <  5:353208/149‑1 (MQ=255)
                                                                                                           cccAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGGCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAg                                      <  1:313433/149‑1 (MQ=255)
                                                                                                            ccAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGTGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGCCGAGGCCTATCGCTACGATGCCAGCGCCAACCTGagt                                            >  4:303510/1‑140 (MQ=255)
                                                                                                            ccAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGTGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGagt                                            >  4:308608/1‑140 (MQ=255)
                                                                                                            ccAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGTGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGAtt                                            <  3:308608/142‑1 (MQ=255)
                                                                                                            ccAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGTGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGAtt                                            <  3:303510/142‑1 (MQ=255)
                                                                                                            ccAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAgg                                     >  6:107206/1‑149 (MQ=39)
                                                                                                              aaGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTa                                   <  3:129018/149‑1 (MQ=39)
                                                                                                                 gCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACt                                 >  4:236965/1‑148 (MQ=39)
                                                                                                                         gTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTgacccaga                         <  3:105836/148‑1 (MQ=39)
                                                                                                                            aCCCGCACGCGCTACGACACCAGGGGTCGGCTGGCCGGAAAGGCTATTCAGTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGATGGACTAGGCCTATCGCTACGATGCCAGCGACAACCTGATTTCCGa                                       <  3:209467/131‑1 (MQ=11)
                                                                                                                            aCCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTgacccagaccca                     >  5:57127/1‑149 (MQ=255)
                                                                                                                            aCCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTgacccagacc                       >  6:347339/1‑147 (MQ=40)
                                                                                                                            aCCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATCCACCTCCTGAAGTGCTGCCACTCCTGTACCAGGCCTATCGCTACGATGCCAGAGACAACCTGAGTGCCGa                                       >  4:209467/1‑131 (MQ=11)
                                                                                                                              ccGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTga                               >  2:22976/1‑137 (MQ=40)
                                                                                                                              ccGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTga                               <  1:22976/137‑1 (MQ=40)
                                                                                                                              ccGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAgc                   >  7:119648/1‑149 (MQ=255)
                                                                                                                                    gcgcTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCcgcg             <  5:107206/149‑1 (MQ=255)
                                                                                                                                      gcTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACACAGCGCCGCgg            >  4:36847/1‑148 (MQ=255)
                                                                                                                                        tACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCCGCGGGAt         >  8:169243/1‑149 (MQ=255)
                                                                                                                                          cGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCCGCGGGATGa       <  5:366452/149‑1 (MQ=255)
                                                                                                                                           gACACCAGCGGTCGCCTGGCCTGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCCGCTGGATGAc      <  2:208655/149‑1 (MQ=255)
                                                                                                                                               ccAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCCGCGGGATGACCAAc  <  7:73225/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ACTCAACCACCTGTACTACGGCAGCGGGCACCTGCACCAGATCAACCTGAACGGCCGGGTGATCAGCGATTTCGAACGTGATGCGGTGCACGACGAGGTCCTGCGCACCCAAGGCAAACTGGTCACCCGCACGCGCTACGACACCAGCGGTCGCCTGGCCGGAAAGGCTATTCACTACCGCGATGCCCCTGCTGAAGTGCTGCCACTGCTGGACGAGGCCTATCGCTACGATGCCAGCGACAACCTGATTGCCGAGGTACTGACCCAGACCCAGCGCCGCGGGATGACCAAC  >  NC_002947/4622853‑4623144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑