Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,816 T→G 100% intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG100.0% 130.3 / ‑6.8 40intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (21/18);  total (21/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCA  >  NC_002947/4740679‑4740951
                                                                                                                                         |                                                                                                                                        
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                <  8:69128/147‑1 (MQ=255)
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                              <  1:175511/149‑1 (MQ=255)
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                              <  8:339595/149‑1 (MQ=255)
 aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                             >  1:311032/1‑149 (MQ=255)
  gCCCGTACAAGATTGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCct                                                                                                                            >  8:176402/1‑149 (MQ=255)
    ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                          <  7:340178/149‑1 (MQ=255)
     cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCtt                                                                                                                         >  7:129072/1‑149 (MQ=255)
         cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                                     >  6:260224/1‑149 (MQ=255)
           aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc                                                                                                                   <  2:311032/149‑1 (MQ=255)
                       cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGaa                                                                                                       <  6:34476/148‑1 (MQ=38)
                         ggcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                     <  1:58777/148‑1 (MQ=38)
                         cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                     <  3:236583/149‑1 (MQ=255)
                          gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                    <  7:176402/149‑1 (MQ=38)
                            cAGCTGTACACCGGCGAGCAGAACCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTCGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAAGAGGc                                                                                                  <  1:142056/149‑1 (MQ=37)
                                   acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                          <  4:1975/118‑1 (MQ=255)
                                   acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                          >  3:1975/1‑118 (MQ=255)
                                     acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAg                                                                                          >  3:292348/1‑148 (MQ=255)
                                           gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                    <  5:274112/132‑1 (MQ=255)
                                           gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                    >  6:274112/1‑132 (MQ=255)
                                             gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TGTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACCGGCACccca                                                                                  >  8:146251/1‑148 (MQ=255)
                                               aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                      >  6:290736/1‑125 (MQ=37)
                                               aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                      <  5:290736/125‑1 (MQ=37)
                                               aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGTAGAGGCCGGCACAGACACCACaaaa                                                                               >  2:243362/1‑149 (MQ=255)
                                                 aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                      <  6:147623/124‑1 (MQ=37)
                                                 aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                      >  5:147623/1‑124 (MQ=37)
                                                       gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCCCCGACACCACAAAACCTCA‑Gtt                                                                      >  3:174876/1‑149 (MQ=255)
                                                                            cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAg                                                 >  6:95965/1‑149 (MQ=255)
                                                                              cAATTACGCTGCAGTAGCCGAACGCAAGAAGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGCCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGcc                                               <  7:146251/149‑1 (MQ=255)
                                                                                  tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCACAGCCCCCACAAAACCGCA‑GGTGTTCTCCGCCCGCTCACGCAGcccgtg                                           >  2:120239/1‑146 (MQ=255)
                                                                                       tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCATGCCCGCGAAGAGGGCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGACCCCTCACGCAGCCCTTTCAggg                                      >  5:153905/1‑149 (MQ=255)
                                                                                            tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                  >  6:3279/1‑148 (MQ=255)
                                                                                               ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTGATCGGGGGCTTGGCCGGGGAGGGGGCGGGCCAGCCCCCCCAAAACCGGC‑GGTTTTGGGCGGCCGCGGACGCCGCCCGTTGCGGGTATTGAAc                              >  2:93509/1‑149 (MQ=255)
                                                                                                 gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                              <  7:231083/147‑1 (MQ=255)
                                                                                                 gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                              >  8:231083/1‑147 (MQ=255)
                                                                                                  aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaa                                                                                >  4:273699/1‑97 (MQ=37)
                                                                                                  aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaa                                                                                <  3:273699/97‑1 (MQ=37)
                                                                                                     ccacgatggCTGCCCGCGTGCTG‑CTTTTTTTTTTTGGCCGGTGCGGGCCTCTGCGCGGCATTGCCCGCTATGTGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                        <  8:198430/142‑1 (MQ=255)
                                                                                                           atgCTGCCCTCCGGCATCCTTTTTTTTTTGTCCTTG‑GCCGGCCGCATCGGGGCCTTGCCCGGGAATAGGCCGGGAAATTAAACAAAAAACCTCATCTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                   <  1:93509/147‑1 (MQ=255)
                                                                                                                  ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTATCCGCCCGCTCACGCAGCCCGTTCAGGGTATTGAACGGCGCATCCACCACGAAcg           >  2:7092/1‑148 (MQ=255)
                                                                                                                            gccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca  >  6:343702/4‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCA‑GTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCA  >  NC_002947/4740679‑4740951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑