Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,745,138	A→T	14.8%	L13H (CTC→CAC)	PP_4199 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,745,138	0	A	T	14.8%	147.4 / 14.7	66	L13H (CTC→CAC)	PP_4199	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (30/26);  new base T (6/4);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGT  >  NC_002947/4744991‑4745286                                                                                                                                                     |                                                                                                                                                     gACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGa                                                                                                                                                      <  5:37492/148‑1 (MQ=255)  aCTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCGTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGc                                                                                                                                                    <  8:273490/149‑1 (MQ=255)   cTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCATCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAg                                                                                                                                                     >  1:265342/1‑148 (MQ=255)         gcgGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCa                                                                                                                                             <  1:243931/149‑1 (MQ=255)          cgGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                            <  2:79832/149‑1 (MQ=255)             gCCAGCTTGTCTTCAGCCAGTTTCAGCGTTGCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAgg                                                                                                                                           <  5:172361/147‑1 (MQ=255)             gCCAGCTTGTCTTCAGCCAGTTTCAGCGTGTCGCCCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCGCTTCAGGGGCCGGATCATTGGCGCAGCCCCCCAGGCCGAGCCCGGCCAggg                                                                                                                                          >  8:99792/1‑148 (MQ=255)              ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGc                                                                                                                                         >  1:99393/1‑148 (MQ=255)                 gCTTCTCTTAAGCCAGTTTCAGCGTTTCGACCTGC‑TGAGTGGCGCCGACGGCCTTGGCCTGGACCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAgg                                                                                                                                     <  5:283592/149‑1 (MQ=255)                   gggTCTTCAGCCAGTTTCAGCGTTTCGACCTGG‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATGTGCTCTTCAGGGGCCGGAGCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGAt                                                                                                                                   <  7:99792/147‑1 (MQ=255)                        ttCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTg                                                                                                                              >  7:301959/1‑149 (MQ=255)                             ccAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                         <  2:99393/149‑1 (MQ=255)                               aGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGt                                                                                                                       <  8:121293/149‑1 (MQ=255)                                     aGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCAc                                                                                                                 <  7:336977/149‑1 (MQ=255)                                      ccGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCCCATCTGCTCTTCAGCGTCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACg                                                                                                                >  7:233959/2‑149 (MQ=255)                                                   c‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAgt                                                                                                   <  8:135067/149‑1 (MQ=255)                                                     tCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTc                                                                                                        <  6:10272/143‑1 (MQ=255)                                                     tCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTc                                                                                                        >  5:10272/1‑143 (MQ=255)                                                       aGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                         >  5:134198/1‑124 (MQ=255)                                                        gTGGCTCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGTAATGCTCATGTGCTCTTCTGGGGCCGTATCATTGGCGCAGCCAGGCTGGCCGAGCACGGCCAGGGGAAGGATTAGTGGTTTGGTTCTCACGCAATCTTCCTAGTGttt                                                                                               <  3:175689/149‑1 (MQ=255)                                                           gcgcCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTggg                                                                                            >  5:311980/1‑149 (MQ=255)                                                                    gCCTTGGCCTGGCCCAGTGCCTGGTCGGAAATGCGCATCTGCTCTTCAGCGGCCGGAGCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                   <  8:301959/149‑1 (MQ=255)                                                                     ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGa                                                                                                                                    <  6:24110/99‑1 (MQ=255)                                                                     ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGa                                                                                                                                    >  5:24110/1‑99 (MQ=255)                                                                      cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgccg                                                                                 >  8:57705/1‑149 (MQ=255)                                                                           ccTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGTCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGt                                                                                       >  6:69236/1‑138 (MQ=255)                                                                           ccTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGTCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGt                                                                                       <  5:69236/138‑1 (MQ=255)                                                                           ccTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAcc                                                                            >  7:37578/1‑149 (MQ=255)                                                                           ccTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAcc                                                                            >  1:86329/1‑149 (MQ=255)                                                                           ccTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAcc                                                                            >  6:9236/1‑149 (MQ=255)                                                                            cTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCt                                                                           >  5:309930/1‑149 (MQ=255)                                                                            cTGGTCCAGCGCCGCTTCGGAAATCCCCCTCAGCGCTTCACGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGGTGGGTTCTCACGCAATCTTCCTCGTGTTTGGGGCCGTCCGCCGGTACCt                                                                           <  8:233959/149‑1 (MQ=255)                                                                             tGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgggg                                                                                           >  5:160971/1‑132 (MQ=255)                                                                             tGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgggg                                                                                           <  6:160971/132‑1 (MQ=255)                                                                             tGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTg                                                                          >  8:253015/1‑149 (MQ=255)                                                                               ttCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGc                                                                         <  4:221749/147‑1 (MQ=255)                                                                               gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGc                                                                         <  7:176805/148‑1 (MQ=255)                                                                               gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCa                                                                        >  5:223610/1‑149 (MQ=255)                                                                               gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCa                                                                        >  5:254302/1‑149 (MQ=255)                                                                                    gTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgc                                                                   <  5:9236/149‑1 (MQ=255)                                                                                       ccTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGACAGCCAGGCCGAGCACCGCCAGGGCAAGGAATAGTGGTTGGGTTCTCACCCAAACTTACTACTGATTTGGGGCGTACCCCGGAAACAGCATCttagca                                                                >  1:88888/1‑144 (MQ=255)                                                                                                  aTGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTc                                                     <  2:265342/149‑1 (MQ=255)                                                                                                    gcgcATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGa                                                   >  8:148408/1‑149 (MQ=255)                                                                                                        aTCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCt                                                                           <  2:293158/121‑1 (MQ=255)                                                                                                        aTCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTACTGTTTGGGGGCGTCCGCCGGTACCt                                                                           >  1:293158/1‑121 (MQ=255)                                                                                                          cTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTc                                             >  1:322478/1‑149 (MQ=255)                                                                                                            gCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCATGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGt                                           >  4:289268/1‑149 (MQ=255)                                                                                                             ctctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTc                                             <  6:309930/146‑1 (MQ=255)                                                                                                                ttCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTg                                         <  6:311980/147‑1 (MQ=255)                                                                                                                ttCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTg                                         >  8:70646/1‑147 (MQ=255)                                                                                                                          ggATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTc                             <  2:86329/149‑1 (MQ=255)                                                                                                                           gATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCg                            >  5:345376/1‑149 (MQ=255)                                                                                                                               aTTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATg                        >  2:39247/1‑149 (MQ=255)                                                                                                                                          tgccctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCa                   >  4:249122/7‑143 (MQ=255)                                                                                                                                          cagccagGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTGTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCa                   >  7:239786/1‑143 (MQ=255)                                                                                                                                            ccctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCaga                 <  7:322761/139‑1 (MQ=255)                                                                                                                                            ccctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCaga                 >  8:322761/5‑143 (MQ=255)                                                                                                                                             cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTGGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCgg           >  7:323063/4‑148 (MQ=255)                                                                                                                                             cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCgg           >  3:337307/4‑148 (MQ=255)                                                                                                                                             cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGc          <  3:249122/146‑1 (MQ=255)                                                                                                                                              ctggCCGTGCTCGGCCTGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCAc                     <  5:1241/135‑1 (MQ=255)                                                                                                                                              ctggCCGTGCTCGGCCTGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCAc                     >  6:6378/3‑137 (MQ=255)                                                                                                                                              ctggCCGTGCTCGGCCTGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCAc                     <  5:6378/135‑1 (MQ=255)                                                                                                                                              ctggCCGTGCTCGGCCTGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCAc                     >  6:1241/3‑137 (MQ=255)                                                                                                                                               agGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCaa        >  6:6656/1‑149 (MQ=255)                                                                                                                                                    gAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTg   >  4:90434/1‑149 (MQ=255)                                                                                                                                                    gAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTg   <  8:37578/149‑1 (MQ=255)                                                                                                                                                     aGCACGGCCAGGGCAAGGATTAGTGGTTTCGATCTCACGCAATCTTCCTACTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGt  <  6:223610/149‑1 (MQ=255)                                                                                                                                                     aGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGt  <  6:254302/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGC‑TCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGT  >  NC_002947/4744991‑4745286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑