Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,748,486	T→C	6.5%	F64L (TTC→CTC)	PP_4203 →	ubiquinone oxidoreductase electron transfer flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,748,486	0	T	C	6.5%	170.6 / 2.2	62	F64L (TTC→CTC)	PP_4203	ubiquinone oxidoreductase electron transfer flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (26/31);  new base C (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

GACGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGCCCAAGACCAT  >  NC_002947/4748339‑4748634                                                                                                                                                    |                                                                                                                                                      gACGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGtt                                                                                                                                                      >  4:235423/1‑149 (MQ=255)  aCGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTc                                                                                                                                                     <  2:118907/149‑1 (MQ=255)   cGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTc                                                                                                                                                     >  5:168906/1‑148 (MQ=255)   cGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCg                                                                                                                                                    <  8:287364/149‑1 (MQ=255)   cGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCg                                                                                                                                                    <  6:168906/149‑1 (MQ=255)          aTCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCCCATCCTCTCTGGCGCAGTGTTCGAGCCCcg                                                                                                                                             >  3:265129/1‑149 (MQ=255)                 gcgcCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCt                                                                                                                                       >  5:99742/1‑148 (MQ=255)                     cggcccggcGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGTGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACg                                                                                                                                  <  8:188800/149‑1 (MQ=255)                       gcccggcGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGcc                                                                                                                                         >  7:28995/1‑140 (MQ=255)                       gcccggcGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGcc                                                                                                                                         <  8:28995/140‑1 (MQ=255)                          cggcggGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTg                                                                                                                             <  3:261868/149‑1 (MQ=255)                               ggCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTccc                                                                                                                        <  8:270913/149‑1 (MQ=255)                               ggCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCGGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTccc                                                                                                                        <  6:306017/149‑1 (MQ=255)                               ggCCGGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGGAGCGAAATCAGCGTCTGCGTGGGGGAGAAAGGCGCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTccc                                                                                                                        <  5:41131/149‑1 (MQ=255)                                      ccgccgCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑Tgg                                                                                                                <  8:316409/149‑1 (MQ=255)                                               gccgcctgAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTc                                                                                                       >  8:129200/1‑149 (MQ=255)                                                ccgcctgAAGCAGAAGGCCGCGGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGACAGGCTCTGAAGTCGGCGCCGACATCCACTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGCGCTGTTCGCCGCC‑TGGAAAGAGCTCg                                                                                                      >  6:105431/1‑149 (MQ=255)                                                 cgcctgAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGAGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCgg                                                                                                     <  5:41146/149‑1 (MQ=255)                                                   cctgAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTg                                                                                                   <  7:129200/149‑1 (MQ=255)                                                       aaGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCa                                                                                                 <  4:222099/147‑1 (MQ=255)                                                         gCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGc                                                                                             >  5:354220/1‑149 (MQ=255)                                                           aGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCt                                                                                            >  7:34203/1‑148 (MQ=255)                                                             aaGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGaa                                                                                         >  5:242476/1‑149 (MQ=255)                                                             aaGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGaa                                                                                         >  5:247738/1‑149 (MQ=255)                                                                  cgccgAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCg                                                                                    <  5:264367/149‑1 (MQ=255)                                                                   gccgAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCGACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCg                                                                                    >  8:24189/1‑148 (MQ=255)                                                                    ccgAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAGGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCg                                                                                    >  8:164570/1‑147 (MQ=255)                                                                    ccgAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGATCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGaa                                                                                  <  3:235423/149‑1 (MQ=255)                                                                    ccgAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCAGTGCACCGCTGAACACCGaa                                                                                  <  6:198222/149‑1 (MQ=255)                                                                     cgAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAg                                                                                 <  6:99742/149‑1 (MQ=255)                                                                         gCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGaa                                                                             <  6:6310/149‑1 (MQ=255)                                                                                 cGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGcgac                                                                      >  5:110414/1‑148 (MQ=255)                                                                                   aaaCCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCgacgac                                                                   <  7:231748/149‑1 (MQ=255)                                                                                    aaTCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACa                                                                  <  8:248780/149‑1 (MQ=255)                                                                                         gCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             >  2:120986/1‑149 (MQ=255)                                                                                            tCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTa                                                          <  1:120986/149‑1 (MQ=255)                                                                                                cGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTc                                                       <  1:122304/148‑1 (MQ=255)                                                                                                 gtggtgGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCaa                                                     <  6:242476/149‑1 (MQ=255)                                                                                                 gtggtgGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCaa                                                     <  6:247738/149‑1 (MQ=255)                                                                                                       gagaAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGc                                               >  1:254601/1‑149 (MQ=255)                                                                                                        agaAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGcc                                              >  5:32589/1‑149 (MQ=255)                                                                                                          aaaGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCgg                                            <  7:178121/149‑1 (MQ=255)                                                                                                           aaGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCTCCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             >  2:348301/1‑131 (MQ=255)                                                                                                           aaGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             <  1:348301/131‑1 (MQ=255)                                                                                                           aaGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGc                                           >  3:189056/1‑149 (MQ=255)                                                                                                                tctGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAgcgc                                      >  7:264227/1‑149 (MQ=255)                                                                                                                       tCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAg                               <  6:19890/149‑1 (MQ=255)                                                                                                                                acaTCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGAc                      <  6:354220/149‑1 (MQ=255)                                                                                                                                acaTCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGAc                      >  1:327042/1‑149 (MQ=255)                                                                                                                                     ctctctGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGGCGTGGAAAGAGCGCGGTGCACCCCTGAACCCCGAAGTGAAGCCCGACGCCATCTACGTACTCAAGGATGCCGCCAGCCCGACCAAGGTGCCTGCCATGt                  >  1:84512/1‑149 (MQ=255)                                                                                                                                        tctGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCgacgac                                                                   >  7:306650/1‑96 (MQ=255)                                                                                                                                        tctGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCgacgac                                                                   <  8:306650/96‑1 (MQ=255)                                                                                                                                          tGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAg                                                    >  8:166841/1‑109 (MQ=255)                                                                                                                                          tGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAg                                                    <  7:166841/109‑1 (MQ=255)                                                                                                                                          tGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGc            >  2:316022/1‑149 (MQ=255)                                                                                                                                          gggCGCGGGGCTCGAACACTGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             >  8:84393/2‑100 (MQ=255)                                                                                                                                          gggCGCGGGGCTCGAACACTGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             >  8:79515/2‑100 (MQ=255)                                                                                                                                          gggCGCGGGGCTCGAACACTGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             <  7:84393/99‑1 (MQ=255)                                                                                                                                          gggCGCGGGGCTCGAACACTGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTAc                                                             <  7:79515/99‑1 (MQ=255)                                                                                                                                            gcgcAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGcc           <  2:221687/148‑1 (MQ=255)                                                                                                                                               cAGAGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTCAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGCATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGccc          >  4:327472/1‑146 (MQ=255)                                                                                                                                                aGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGCCCAAGa      >  5:315638/1‑149 (MQ=255)                                                                                                                                                aGTGGTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAACGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGCCCAAGa      <  4:265129/149‑1 (MQ=255)                                                                                                                                                  tgtTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGCCCAAGAc     >  3:66879/1‑148 (MQ=255)                                                                                                                                                    ttCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGCCCAAGACCAt  >  6:196090/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GACGTGGTCATCGTCGGCGCCGGCCCGGCGGGCCTGTCCGCCGCCTGCCGCCTGAAGCAGAAGGCCGCCGAAGCCGGTAGCGAAATCAGCGTCTGCGTGGTGGAGAAAGGCTCTGAAGTCGGCGCCCACATCCTCTCTGGCGCAGTGTTCGAGCCCCGCGCCCTGAACGAGCTGTTCCCCGAC‑TGGAAAGAGCTCGGTGCACCGCTGAACACCGAAGTGAAGCGCGACGACATCTACGTACTCAAGGATGCCGGCAGCGCGACCAAGGTGCCTGACCTGTTCGTGCCCAAGACCAT  >  NC_002947/4748339‑4748634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑