Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,796,733 C→G 5.4% G417A (GGT→GCT)  PP_4224 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,7330CG 5.4% 244.6 / 3.3 74G417A (GGT→GCT) PP_4224sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base G (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

GCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAGCCCACCTG  >  NC_002947/4796590‑4796881
                                                                                                                                               |                                                                                                                                                    
gCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGca                                                                                                                                                 >  3:163288/1‑149 (MQ=255)
gCCGCTTCGCGGGTAAACCCGCCCGTAGCAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg                                                                                                                                                   <  2:268287/147‑1 (MQ=255)
  ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAACCCCGAAGAGGCCAGCACAGGCGATAAACATAAATGCCCAGGACCCGCCGGTACCAGCAACAAAAACCGCAAGCCGTAACTACCGCTGTCAAATCGCAGGCTACCGGcaca                                                                                                                                               >  2:166703/1‑149 (MQ=255)
           ggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                                                                                                                                      >  2:304925/1‑149 (MQ=38)
           ggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                                                                                                                                      >  8:295070/1‑149 (MQ=255)
             tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGACGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACAACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa                                                                                                                                    >  5:310988/3‑149 (MQ=37)
               aaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc                                                                                                                                  <  1:304925/149‑1 (MQ=38)
                aCCCGCCCGTAGGAGCGGGTTCACGCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCg                                                                                                                                 >  6:371551/1‑149 (MQ=255)
                 cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc                                                                                                                                >  4:248081/1‑149 (MQ=38)
                 cccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc                                                                                                                                >  4:37609/1‑149 (MQ=255)
                  ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc                                                                                                                               >  2:236904/1‑149 (MQ=255)
                    gcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTg                                                                                                                             >  1:54139/1‑149 (MQ=255)
                     cccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGc                                                                                                                            <  7:295070/149‑1 (MQ=255)
                            gAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt                                                                                                                              >  3:8584/1‑140 (MQ=255)
                            gAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt                                                                                                                              <  4:8584/140‑1 (MQ=255)
                               cGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCa                                                                                                                  <  3:37609/149‑1 (MQ=255)
                                ggggTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCTGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAg                                                                                                                 >  7:242446/1‑149 (MQ=255)
                                 ggTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCTGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGc                                                                                                                <  8:242446/149‑1 (MQ=255)
                                        ccGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAAc                                                                                                         <  1:236904/149‑1 (MQ=255)
                                           cgAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCg                                                                                                       >  7:86605/1‑148 (MQ=255)
                                              agagGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAac                                                                                                   <  8:86605/149‑1 (MQ=255)
                                              agagGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGACGTCACTACGGCTGTCAACGCGCAGGCTACCGGCACACAGCTGCACGATCGCCTGCACAATCGCCAGCCCAAAACCGCAac                                                                                                   >  3:57347/1‑149 (MQ=255)
                                                 ggCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAaccac                                                                                                <  5:371551/149‑1 (MQ=255)
                                                 ggCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAaccac                                                                                                <  4:114110/149‑1 (MQ=255)
                                                  gCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa                                                                                                                                       <  1:5760/109‑1 (MQ=255)
                                                  gCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa                                                                                                                                       >  2:5760/1‑109 (MQ=255)
                                                         caGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGc                                                                                        <  1:166703/149‑1 (MQ=255)
                                                         caGACGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGc                                                                                        <  6:310988/149‑1 (MQ=255)
                                                              gATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCAtt                                                                                   <  6:294705/149‑1 (MQ=255)
                                                                     aTCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTgcgc                                                                               >  1:55553/1‑146 (MQ=255)
                                                                     aTCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTgcgc                                                                               >  3:138243/1‑146 (MQ=255)
                                                                       cAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGcccc                                                                                                             >  6:103739/1‑114 (MQ=255)
                                                                       cAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGcccc                                                                                                             <  5:103739/114‑1 (MQ=255)
                                                                            cccAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCt                                                                     >  6:360931/1‑149 (MQ=255)
                                                                            cccAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCt                                                                     >  4:340587/1‑149 (MQ=255)
                                                                              cAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCg                                                                   <  4:163288/149‑1 (MQ=255)
                                                                              cAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCg                                                                   <  5:272932/149‑1 (MQ=255)
                                                                                 gACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGt                                                                 >  2:16705/1‑148 (MQ=255)
                                                                                   cgcgCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAg                                                              <  1:16705/149‑1 (MQ=255)
                                                                                      gcCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTg                                                           >  4:289883/1‑149 (MQ=255)
                                                                                            aCCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAg                                                     >  5:190195/1‑149 (MQ=255)
                                                                                              cTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGt                                                   >  8:66364/1‑149 (MQ=255)
                                                                                               tGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACCCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTc                                                  >  8:23034/1‑149 (MQ=255)
                                                                                                 caacaaAACCCGCAAGCCTTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 <  4:57347/148‑1 (MQ=255)
                                                                                                  aacaaAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGc                                               <  5:360931/149‑1 (MQ=255)
                                                                                                  aacaaAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGc                                               <  3:340587/149‑1 (MQ=255)
                                                                                                   acaaAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGcc                                              >  3:80361/1‑149 (MQ=255)
                                                                                                    caaAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCt                                             <  3:248081/149‑1 (MQ=255)
                                                                                                      aaaCCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCa                                           <  4:138243/149‑1 (MQ=255)
                                                                                                       aaCCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAg                                          <  7:66364/149‑1 (MQ=255)
                                                                                                         cccGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCACTCGGTCAGGTGCTCCAGGTCCGCCTCAGc                                         >  1:154936/1‑148 (MQ=255)
                                                                                                          ccGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCAt                                                                                    >  8:74/1‑104 (MQ=255)
                                                                                                          ccGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCAt                                                                                    <  7:74/104‑1 (MQ=255)
                                                                                                                 ccGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 <  4:315862/132‑1 (MQ=255)
                                                                                                                 ccGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 >  3:212723/1‑132 (MQ=255)
                                                                                                                 ccGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 <  4:321024/132‑1 (MQ=255)
                                                                                                                 ccGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 >  3:315862/1‑132 (MQ=255)
                                                                                                                 ccGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 >  3:321024/1‑132 (MQ=255)
                                                                                                                 ccGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTcc                                                 <  4:212723/132‑1 (MQ=255)
                                                                                                                       cTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATg                          <  3:289883/149‑1 (MQ=255)
                                                                                                                       cTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATg                          >  7:29632/1‑149 (MQ=255)
                                                                                                                            gCTGTCAAAGCGCAGGCTAGCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCt                                             <  1:136020/125‑1 (MQ=255)
                                                                                                                            gCTGTCAAAGCGCAGGCTAGCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCt                                             >  2:130674/1‑125 (MQ=255)
                                                                                                                            gCTGTCAAAGCGCAGGCTAGCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCt                                             >  2:136020/1‑125 (MQ=255)
                                                                                                                            gCTGTCAAAGCGCAGGCTAGCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCt                                             <  1:130674/125‑1 (MQ=255)
                                                                                                                            gCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACCCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCAc                     <  7:23034/149‑1 (MQ=255)
                                                                                                                                   aaGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCAt              <  2:55553/149‑1 (MQ=255)
                                                                                                                                      cgcAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAAt                                      <  5:105594/122‑1 (MQ=255)
                                                                                                                                      cgcAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAAt                                      >  6:105594/1‑122 (MQ=255)
                                                                                                                                       gcAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAg          <  6:190195/149‑1 (MQ=255)
                                                                                                                                        cAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAGc         <  5:290666/149‑1 (MQ=255)
                                                                                                                                         aGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAGcc        >  2:149499/1‑149 (MQ=255)
                                                                                                                                            cTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTgc                                                                                 >  7:73957/1‑73 (MQ=255)
                                                                                                                                            cTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTgc                                                                                 <  8:73957/73‑1 (MQ=255)
                                                                                                                                               ccGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCACCCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTAAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAGCCCACCTg  >  6:225605/1‑149 (MQ=255)
                                                                                                                                               ccGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAGccgcgctg  >  7:65954/1‑143 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCCCAAACCGCAACCACCACTCTGCCCATTGCGCCAGAAGCGCTCGGTCAGGTGCTCCAGGTCCGCCTCAGCAATGCCTGGCCCATGGTCACGCACCATGAAGCCCACCTG  >  NC_002947/4796590‑4796881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑