| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | 100% | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 100.0% | 53.6 / NA | 22 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (12/10); total (12/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 58.4 / NA | 22 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (12/10); total (12/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTC > NC_002947/1499358‑1499626 || aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAc > 8:257881/1‑149 (MQ=37) gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCCCAGACTg > 3:244271/1‑149 (MQ=21) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCAtt > 1:106391/1‑148 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCgg < 1:20102/149‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGc > 1:235451/1‑149 (MQ=21) ctttGGAACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGGGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCGGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAg < 6:287929/134‑1 (MQ=18) ccTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCCCGCC‑CGCTCCCACAGACTGATTGCCaattag > 5:287929/1‑131 (MQ=18) ggCACCACCGCAGGACTGAGGCG‑GCCAAACGGGGGGCGGGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 4:305587/148‑1 (MQ=21) caccacAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg < 2:235451/149‑1 (MQ=21) tGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CCCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGGGACGGGCCGtttttt > 4:268977/1‑149 (MQ=21) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCATGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 7:201203/1‑149 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt > 3:92205/1‑130 (MQ=21) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 4:92205/130‑1 (MQ=21) gCGGGCGTCCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 4:219995/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt < 7:335566/149‑1 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:361276/1‑149 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:100580/1‑146 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATAGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 2:127222/1‑148 (MQ=37) gAAACAGGCGCCGCGGTGCATGGAACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACATACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc < 7:257881/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 1:327843/149‑1 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa < 2:106391/149‑1 (MQ=37) accGGCTTTGCCGGTGTTCGCGGGCACG‑CTCGCTCCCACAGACTGATTTCCATTAAGCGGGCCTATCGCTTCAGTGACGGCCAGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 1:265751/3‑149 (MQ=38) || AACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCT‑GCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTC > NC_002947/1499358‑1499626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |