Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	38.0 / NA	14	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (4/10);  total (4/10)

TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG  >  NC_002947/1499383‑1499646                                                                                                                               |                                                                                                                                             ttCAGAGCCTGAACGAAGCCTTGCCACCACAGCAGGACTGCGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGcc                                                                                                                        <  5:58459/148‑1 (MQ=21)     gcgCCTGACCGACGCCTGGGCACCACCGCCGGCCGGAGGCTGCCAAACGGGGGGCGCGGGCGGGCCCGCGAAACAGGCGCCGCGGGGCAGGGCACGGGCTTTGCCGGTGTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCAtt                                                                                                                     <  3:76334/145‑1 (MQ=16)          tGAACGAAGCCTTGGCACCACAGCAGGACTGCGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                               <  6:151934/148‑1 (MQ=21)                        ccaccccCGCCGGAGTGCGGCTGCAAAAGGGGGGGCGGGGGGGTGCCCGCTAAACTGGCGCGGGGGTGCATGGCACGGGCTTGGCGGGTGTGC‑GCGGCCAGGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                                                                  <  8:140325/146‑1 (MQ=255)                                           gcggCCAAACGGGGGGCGCGGGCGGGCCCGCGAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGCCGGGGTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                              <  3:142570/145‑1 (MQ=21)                                               ccAAACGGTGGGAGCGGGCCTGCCCGCGAAACACGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCCCGCCCGCGCCCCCAGCCTGATTGCCATTAAGCGGGCCGATCGCTACCGTGGCCGCCCGGTTTTTGTTGCCt                                                                          >  6:132062/1‑148 (MQ=17)                                                               ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                          <  5:33388/148‑1 (MQ=37)                                                                   tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                                      >  8:108235/1‑148 (MQ=25)                                                                          gAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                                               <  5:87539/148‑1 (MQ=37)                                                                               aGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGTGC‑GCGGGAACGCCCGCTCCCACAGACTTATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                          <  1:10920/148‑1 (MQ=37)                                                                                        ggTGCATGGCACGGGCTTTGCGGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa                                 <  4:165874/148‑1 (MQ=37)                                                                                        ggTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa                                 <  4:27101/148‑1 (MQ=37)                                                                                                                     cgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc     >  1:79083/3‑148 (MQ=255)                                                                                                                        gggCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg  >  2:151972/1‑148 (MQ=255)                                                                                                                               |                                                                                                                                             TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG  >  NC_002947/1499383‑1499646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑