| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 94.1% | 48.1 / ‑2.6 | 17 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (10/6); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
TCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932512‑1932774 | tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCCGGCTT‑GCCCGCGAAGGGCTGCAAGGcc > 2:8364/1‑147 (MQ=255) tGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGc > 3:50347/1‑148 (MQ=255) ccgCGGCATGGCAGGGCTCACGAAAAGAGCCGTAACCGCGGGGGCCGCTTGGCGGCCCTTCGCGGGCACGCCCGCGCCTACACAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 8:82906/146‑1 (MQ=25) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGGAGCCCCAAATTGTCGACTAcg > 4:17147/1‑148 (MQ=255) ttGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGG‑CCGCGACGGGCTGCAAGGCCGCCCCAAATTGTCGACTAcgc > 8:77546/1‑148 (MQ=255) ccggTCTCACGAAAATCCCAGTAAACGCGGGGGCCGCTTGGCGGCCCTGCGCGGGCAACCCCGCGCCTACACAGGATTTGCGCCCCCTGTCGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCgg < 5:113967/145‑1 (MQ=25) ggctcACTAAAATAGCAGAAACCGGTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCAACAAAGTATAGGCGGCCCCTGTCGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 5:143287/146‑1 (MQ=37) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt > 4:78730/1‑148 (MQ=255) aGCAGAAAACGCTGGGGCCGCTTTGCGGCCGTGCGCGGGCACGCCCGCTCCTACAAAGCATATGCGCCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 3:70219/148‑1 (MQ=38) aagggCCGCAAAGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc > 7:132127/3‑148 (MQ=38) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa > 5:37422/1‑148 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCg > 6:151469/1‑148 (MQ=255) aCAAAGGATAAGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGTAGCCCAGGTATTCCAGCTCGatat > 3:113159/1‑148 (MQ=38) cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 8:80921/1‑147 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg < 8:132127/147‑1 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 8:20842/1‑148 (MQ=255) aGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca < 6:37422/147‑1 (MQ=255) | TCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932512‑1932774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |