| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,035 | +G | intergenic (+118/+97) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,033 | 1 | . | G | 80.0% | 21.8 / 1.2 | 10 | intergenic (+116/+99) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (1/7); total (2/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTG‑C‑GG‑TAAACCCGCTCCTAC‑CAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC > NC_002947/4585889‑4586104 | cgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑C‑ggg < 5:156282/148‑2 (MQ=255) aGCGACCGGTGAAGGATTAAGGGTAGACCACGGCACCTGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGGTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑C‑GGTTAAACCCGc < 4:37941/148‑1 (MQ=255) cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGaa < 3:30798/148‑1 (MQ=39) cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg < 1:77989/148‑1 (MQ=39) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 1:122713/146‑1 (MQ=38) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 7:76001/146‑1 (MQ=38) gcggctCCATTGAAAGGTTGAGGAAGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 1:160911/143‑1 (MQ=38) ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTATTAGAC‑GG‑TAAAACCCCACCAACACAGGGC‑CCGGCCGGCCCTGGAAGCGGCGCAGCCCCCGGTAGGAGCGGGGTTaac > 2:15091/1‑146 (MQ=255) tCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAcc < 2:102540/147‑1 (MQ=38) ttGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGttt > 1:68076/1‑114 (MQ=37) tGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTa < 2:68076/114‑1 (MQ=25) | CGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTG‑C‑GG‑TAAACCCGCTCCTAC‑CAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC > NC_002947/4585889‑4586104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |