Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,498	T→G	intergenic (+75/‑89)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,498	0	T	G	100.0%	26.4 / NA	12	intergenic (+75/‑89)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/10);  total (2/10)

TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGA  >  NC_002947/1499383‑1499615                                                                                                                      |                                                                                                                       ttCAGAGCCTGAACGAAGCCTTGCCACCACAGCAGGACTGCGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGcc                                                                                         <  5:58459/148‑1 (MQ=21)     gcgCCTGACCGACGCCTGGGCACCACCGCCGGCCGGAGGCTGCCAAACGGGGGGCGCGGGCGGGCCCGCGAAACAGGCGCCGCGGGGCAGGGCACGGGCTTTGCCGGTGTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCAtt                                                                                      <  3:76334/145‑1 (MQ=16)          tGAACGAAGCCTTGGCACCACAGCAGGACTGCGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                <  6:151934/148‑1 (MQ=21)                        ccaccccCGCCGGAGTGCGGCTGCAAAAGGGGGGGCGGGGGGGTGCCCGCTAAACTGGCGCGGGGGTGCATGGCACGGGCTTGGCGGGTGTGC‑GCGGCCAGGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                                   <  8:140325/146‑1 (MQ=255)                                           gcggCCAAACGGGGGGCGCGGGCGGGCCCGCGAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGCCGGGGTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                               <  3:142570/145‑1 (MQ=21)                                               ccAAACGGTGGGAGCGGGCCTGCCCGCGAAACACGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCCCGCCCGCGCCCCCAGCCTGATTGCCATTAAGCGGGCCGATCGCTACCGTGGCCGCCCGGTTTTTGTTGCCt                                           >  6:132062/1‑148 (MQ=17)                                                               ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                           <  5:33388/148‑1 (MQ=37)                                                                   tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                       >  8:108235/1‑148 (MQ=25)                                                                          gAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                <  5:87539/148‑1 (MQ=37)                                                                               aGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGTGC‑GCGGGAACGCCCGCTCCCACAGACTTATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa           <  1:10920/148‑1 (MQ=37)                                                                                        ggTGCATGGCACGGGCTTTGCGGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa  <  4:165874/148‑1 (MQ=37)                                                                                        ggTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa  <  4:27101/148‑1 (MQ=37)                                                                                                                      |                                                                                                                       TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGA  >  NC_002947/1499383‑1499615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑