| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 90.5% | 53.0 / ‑5.8 | 21 | G3494R (GGG→AGG) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (13/6); minor base C (0/2); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATT > NC_002947/2921320‑2921604 | ggcggggggCGGCCGGTTACGACAACCTGTACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGc < 6:76258/144‑1 (MQ=255) ggTCACGACCACCTGCTCGGCGGCGGGGGCGAGGACTGCCTGCATGGCGGCAACGGCAATGACAGCCTGGAGGGAGGCGATGGTAACGACACTTTGTGCGGCGACGTGGGTACCGACACCCTGATCAGGCGTGCCGGCAACGACTTcc < 6:125871/148‑1 (MQ=255) aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg < 5:98452/125‑1 (MQ=255) gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACg > 2:60886/1‑148 (MQ=255) ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCATGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCg > 3:140946/1‑148 (MQ=255) cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCg > 2:5056/1‑147 (MQ=255) gcgcGGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGGAACGACAGAGTGGTCGGCGACGGCGGTACCGACACCCTGATCCGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa < 1:7963/148‑1 (MQ=255) gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACAGTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCAGGCAACGACTTCCTCAACGGGGCGGCAGGTAACGAGACggt > 3:41030/1‑148 (MQ=255) gACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTGCGGCGACGTGTGTACCGACACCCTGATCAGGGTGGCCGGCAACGACTTCCTCAACGGTGAGGCAGGTACCGATTCGGtggggg > 6:153019/1‑148 (MQ=255) ccTGGATGGCGGCAACGGCAATGACACCATGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGtgggtggg < 4:140946/148‑1 (MQ=255) cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTgggg > 7:135075/1‑148 (MQ=255) cggcaccgccaaTGCCACCCTGCAGGGCGGCGACGTGACCGACCCGTTGTGCGGCGCCGTGGGTCCCGACACCCTGATCCGGGGGGGCGGCAACGACTTCCTCAACGGGGGGGCAGGTAACGATACGGTGGTGGGGGGGGCCGGTAAc < 2:104684/148‑1 (MQ=255) ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCATCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGTGCCGGTAACGacaaca > 8:128610/1‑148 (MQ=255) cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGGGGGGACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGTTAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGc < 7:128610/147‑1 (MQ=255) tgggCGGCGATGGTAACGACACTTTGTTCGGCGACGTGGGTCCCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt < 8:135075/145‑1 (MQ=255) cGACACATTGTTCGGCGACGTGGGTACCGACACCCTGACCAGGGGGGCCGGCAACGACTTCCTCAACGGGGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa < 1:100956/147‑1 (MQ=255) acaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCg > 6:105063/1‑148 (MQ=255) acacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGa > 4:28164/1‑147 (MQ=255) ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat > 1:29822/1‑147 (MQ=255) cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGAtcatca > 1:42750/1‑148 (MQ=255) tGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCaa > 2:161493/1‑148 (MQ=255) aTCAGGGGGGCCAGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAtt > 1:116851/1‑148 (MQ=255) | TGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATT > NC_002947/2921320‑2921604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |