BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,990,451	T→C	E72E (GAA→GAG)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,990,451	0	T	C	95.8%	1412.0 / 45.8	736	E72E (GAA→GAG)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base T (12/19); new base C (374/325); total (391/345)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 737 total aligned reads displayed.

CCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCA  >  NC_002947/5990306‑5990598

ccACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTc                                                                                                                                                   <  7:148204/148‑1 (MQ=255)

 cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcg                                                                                                                                                  >  1:140431/1‑147 (MQ=255)

 cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAACTCTTTCAGCTcg                                                                                                                                                  >  8:5384/1‑147 (MQ=255)

 cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcg                                                                                                                                                  <  1:119586/148‑2 (MQ=18)

  aCGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCCCCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcgg                                                                                                                                                 >  6:155900/1‑146 (MQ=18)

   cGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGGTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                                                                                                <  8:116966/148‑1 (MQ=18)

   cGCAAACCTCGGTCACAACACCTGCAATGATCAGCGGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGCTCTTTCAGCTCggg                                                                                                                                                <  3:39546/148‑1 (MQ=18)

    gCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCGAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGc                                                                                                                                               <  3:30333/148‑1 (MQ=18)

      aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCa                                                                                                                                              >  5:98278/1‑147 (MQ=18)

        aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCa                                                                                                                                           <  4:19132/148‑1 (MQ=18)

        aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCa                                                                                                                                           >  8:101528/1‑148 (MQ=18)

         ccTCGGTCACACCACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAg                                                                                                                                          >  4:49172/1‑148 (MQ=18)

          cTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGc                                                                                                                                         >  1:26587/1‑148 (MQ=18)

          cTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGc                                                                                                                                         <  8:2785/148‑1 (MQ=18)

          cTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGAGGGCAATGTAAGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCTGc                                                                                                                                         >  8:34218/1‑148 (MQ=18)

           tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACTAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCg                                                                                                                                        <  6:119927/148‑1 (MQ=17)

             ggTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCCGCggg                                                                                                                                      >  5:123609/1‑148 (MQ=17)

              gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTTGGGCACCAGCggg                                                                                                                                      >  4:39562/1‑147 (MQ=17)

              gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCggg                                                                                                                                      >  8:17338/1‑147 (MQ=17)

              gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCgg                                                                                                                                       <  3:8659/146‑1 (MQ=17)

              gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGc                                                                                                                                     <  3:66765/148‑1 (MQ=17)

               tCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGcc                                                                                                                                    >  2:178944/1‑148 (MQ=17)

                 acaaccCCCGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCACTGGACGGCGCAGACGGGACCATCTCGTTCAGCTCGGGCCCCAGCGGGccat                                                                                                                                  >  2:85241/1‑146 (MQ=17)

                 acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGccat                                                                                                                                  >  1:102815/1‑146 (MQ=17)

                 acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGccat                                                                                                                                  >  5:141084/1‑146 (MQ=17)

                 acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGccat                                                                                                                                  >  1:55094/1‑146 (MQ=17)

                  caacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAtt                                                                                                                                 >  3:96346/1‑148 (MQ=17)

                   aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttc                                                                                                                                >  2:7596/1‑147 (MQ=17)

                   aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttc                                                                                                                                >  7:86951/1‑147 (MQ=17)

                   aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTCCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttc                                                                                                                                >  1:152136/1‑147 (MQ=17)

                     cacCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTcgg                                                                                                                              <  7:37693/148‑1 (MQ=17)

                     cacCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTcgg                                                                                                                              <  7:1704/148‑1 (MQ=17)

                      acCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTcggg                                                                                                                             >  2:34195/1‑148 (MQ=17)

                       ccTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGc                                                                                                                            <  7:60361/148‑1 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGATGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCAATTCGGGcc                                                                                                                           >  4:103304/1‑148 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCTGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  3:108263/1‑148 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  6:46455/1‑148 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  6:39741/1‑148 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  5:18977/1‑148 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  8:106342/1‑148 (MQ=17)

                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  1:85961/1‑148 (MQ=17)

                         tGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                                                          >  6:75966/1‑148 (MQ=14)

                          gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         >  3:122055/1‑148 (MQ=14)

                          gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         >  4:7349/1‑148 (MQ=14)

                          gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         >  3:492/1‑148 (MQ=14)

                           cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  4:129126/1‑148 (MQ=14)

                           cAATGATAACCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGCAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCTGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACAAGCACGCCATTCGGGCCTTg                                                                                                                        >  2:608/1‑148 (MQ=14)

                            aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         <  3:155760/146‑1 (MQ=14)

                            aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         <  7:75902/146‑1 (MQ=14)

                            aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                                                                       <  6:89616/148‑1 (MQ=14)

                            aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGAGCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         <  4:64897/146‑1 (MQ=14)

                            aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAATCGGGCCTTGc                                                                                                                       <  5:129334/148‑1 (MQ=14)

                            aaTGATCAGCTGCTTCTTGCCGGGCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        <  8:137739/147‑1 (MQ=14)

                             aTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      <  5:52434/148‑1 (MQ=17)

                              tGATCAGCTGCTTCTTGCCGGTCGCCTTCCCCGCCTTGACGAAATCCTCGTTGTCCCAGGCCTTGATCTGGCCAGGGCGGGCACTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      >  5:66286/1‑147 (MQ=14)

                              tGATCAGCTGCTTCTTGCCGGTCGCCATCACCCCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      >  1:172744/1‑147 (MQ=14)

                               gATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                     >  5:74408/1‑147 (MQ=14)

                               gATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                     >  8:7272/1‑147 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACCTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  >  7:119021/1‑148 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  >  1:131321/1‑148 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  3:143367/148‑1 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  2:25983/148‑1 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  7:107108/148‑1 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  2:98507/148‑1 (MQ=14)

                                 tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  >  4:15922/1‑148 (MQ=14)

                                 gcAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGCCGAAATCCTCGTGGTCCCAGGCGGTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  4:9369/147‑1 (MQ=14)

                                  cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                 <  5:15425/148‑1 (MQ=14)

                                  cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                 <  5:103879/148‑1 (MQ=14)

                                    gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCt                                                                                                               >  6:96936/1‑148 (MQ=14)

                                    gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCt                                                                                                               <  1:70068/148‑1 (MQ=14)

                                     ctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtg                                                                                                              >  2:4687/1‑148 (MQ=14)

                                      tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAACTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                             >  4:145120/1‑148 (MQ=14)

                                      tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGGTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                             <  6:24417/148‑1 (MQ=14)

                                       gctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTCCGGCGCATCCGGGCCCCTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                            >  6:33512/1‑148 (MQ=14)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCCCCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                          >  1:44984/1‑148 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                          <  3:167005/148‑1 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                          <  5:85585/148‑1 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                          <  7:29816/148‑1 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                          >  2:6743/1‑148 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                           <  3:26367/147‑1 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                           <  2:105389/147‑1 (MQ=11)

                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                            <  2:148188/146‑1 (MQ=11)

                                              gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAgg                                                                                                     >  8:90004/1‑148 (MQ=11)

                                              gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGAAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTAGCTCGAAGCTGGTGGTCAgg                                                                                                     >  2:122000/1‑148 (MQ=11)

                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGATCATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                      <  5:35259/146‑1 (MQ=11)

                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAtt                                                                                                                                 <  1:64954/119‑1 (MQ=11)

                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                       <  1:109274/145‑1 (MQ=11)

                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                       <  2:136139/145‑1 (MQ=11)

                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                       <  1:38581/145‑1 (MQ=11)

                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                      <  2:165731/146‑1 (MQ=11)

                                                cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                    <  4:84858/147‑1 (MQ=11)

                                                cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                    <  4:30160/147‑1 (MQ=11)

                                                cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                    <  1:90933/147‑1 (MQ=11)

                                                cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                                                   >  6:141562/1‑148 (MQ=11)

                                                 ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                  <  1:163064/148‑1 (MQ=11)

                                                  gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                 <  3:79887/148‑1 (MQ=11)

                                                           aCCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGt                                                                                        >  7:134039/1‑148 (MQ=255)

                                                                          tCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCa                                                                         <  3:135421/148‑1 (MQ=255)

                                                                                            ttGATCTGGCCAGGGCGGTCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCa                                                       >  8:61643/1‑148 (MQ=255)

                                                                                                                        gcgcATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGat                           <  1:18224/148‑1 (MQ=255)

CCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCA  >  NC_002947/5990306‑5990598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`