| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,030 | 2 bp→TC | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,030 | 0 | C | T | 100.0% | 31.2 / NA | 11 | intergenic (+113/+102) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (2/9); total (2/9) | |||||||||||
| * | NC_002947 | 4,586,031 | 0 | T | C | 90.9% | 27.5 / ‑6.7 | 11 | intergenic (+114/+101) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (1/9); minor base A (1/0); total (2/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTG‑C‑GG‑TAAACCCGCTCCTAC‑CAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC > NC_002947/4585889‑4586104 || cgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑C‑ggg < 5:156282/148‑2 (MQ=255) aGCGACCGGTGAAGGATTAAGGGTAGACCACGGCACCTGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGGTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑C‑GGTTAAACCCGc < 4:37941/148‑1 (MQ=255) cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGaa < 3:30798/148‑1 (MQ=39) cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg < 1:77989/148‑1 (MQ=39) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 1:122713/146‑1 (MQ=38) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 7:76001/146‑1 (MQ=38) gcggctCCATTGAAAGGTTGAGGAAGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 1:160911/143‑1 (MQ=38) ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTATTAGAC‑GG‑TAAAACCCCACCAACACAGGGC‑CCGGCCGGCCCTGGAAGCGGCGCAGCCCCCGGTAGGAGCGGGGTTaac > 2:15091/1‑146 (MQ=255) tCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAcc < 2:102540/147‑1 (MQ=38) ttGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGttt > 1:68076/1‑114 (MQ=37) tGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCG‑CGGG‑TAAACCCGCTCCTAC‑CAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTa < 2:68076/114‑1 (MQ=25) || CGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTG‑C‑GG‑TAAACCCGCTCCTAC‑CAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC > NC_002947/4585889‑4586104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |