Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8050T.95.7% 191.8 / 1.4 48intergenic (+42/+711)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base . (22/23);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTG  >  NC_002947/4740657‑4740947
                                                                                                                                                    |                                                                                                                                                
cGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCt                                                                                                                                                  >  4:8427/1‑149 (MQ=255)
cGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCt                                                                                                                                                  >  5:155371/1‑149 (MQ=255)
    cTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttt                                                                                                                                              <  3:8427/149‑1 (MQ=255)
      ggAAAGAAATGCTCTCCAGCCCGTGCAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCtttttta                                                                                                                                            >  6:146928/1‑148 (MQ=255)
        aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                          <  8:270658/149‑4 (MQ=255)
         aagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttattt                                                                                                                                         <  7:251682/149‑5 (MQ=255)
            aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                      <  2:206867/149‑8 (MQ=255)
                  tctcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                      <  6:195116/143‑8 (MQ=255)
                  tctcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                      >  5:195116/1‑136 (MQ=255)
                    tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGACCGCAAGACGGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                             >  8:249490/1‑149 (MQ=255)
                     ccAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                            >  2:374440/1‑149 (MQ=255)
                     ccAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGT‑AGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTACTGTGCCGGc                                                                                                                           >  1:232549/1‑149 (MQ=255)
                      cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                           <  1:41226/149‑1 (MQ=255)
                      cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                           <  6:59706/149‑1 (MQ=255)
                          ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                       <  2:357637/149‑1 (MQ=255)
                           cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttat                                                                                                                                           <  6:102255/129‑3 (MQ=255)
                           cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttat                                                                                                                                           >  5:102255/1‑127 (MQ=255)
                               cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                             <  8:359963/138‑1 (MQ=255)
                               cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                             >  7:359963/1‑138 (MQ=255)
                                    tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                             >  4:102043/1‑149 (MQ=255)
                                          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                       >  6:139362/1‑149 (MQ=255)
                                             cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                     <  1:374440/148‑1 (MQ=255)
                                             cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                     <  5:278010/148‑1 (MQ=255)
                                              gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                   <  6:155371/149‑1 (MQ=255)
                                               cgcCAGCTGTACTCCTGCCAGCAGAAGGGCGACATCGTTGCCCTGTAAGACCGCATCTAAGCTGCACTAGCCGAAGGCTAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCGTCTTCGCGGCCTTGCCCGCGAaga                                                                                                  <  5:304301/149‑1 (MQ=255)
                                               cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCGCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                 <  5:334652/149‑1 (MQ=38)
                                               cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                  <  5:146928/149‑1 (MQ=38)
                                                        tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca                                                                                          >  2:237204/1‑148 (MQ=255)
                                                        tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTCAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAATAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcac                                                                                         <  6:196617/149‑1 (MQ=255)
                                                          cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAAGAGGCCGGcaca                                                                                        <  6:204310/148‑1 (MQ=37)
                                                           acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGGCGTGGCCGCGAAGAGggcggcgccgg                                                                                      >  1:381326/1‑144 (MQ=255)
                                                                 gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcacca                                                                                 >  3:265639/1‑148 (MQ=255)
                                                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcaccac                                                                                >  2:291707/1‑148 (MQ=255)
                                                                    cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttt                                                                                                                                                <  4:82452/83‑1 (MQ=255)
                                                                    cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttt                                                                                                                                                >  3:82452/1‑83 (MQ=255)
                                                                         gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑t                                                                        >  1:231417/1‑148 (MQ=255)
                                                                              aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGttt                                                                  <  5:139362/149‑1 (MQ=255)
                                                                                   gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGGTTTtctc                                                             >  3:316504/1‑149 (MQ=255)
                                                                                     tGCCCTGAAAGACCGAAAGTAACCTGCAGTACCCTAACCAAACACGGCTGCCCGCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCTCTTCGCGCCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTcc                                                            <  2:249522/148‑1 (MQ=255)
                                                                                       cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAACC‑TCAGATTTTCTacg‑cc                                                        >  3:181700/1‑149 (MQ=255)
                                                                                        ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCtttt                                                                                                                                               <  8:265264/64‑1 (MQ=255)
                                                                                        ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCtttt                                                                                                                                               >  7:265264/1‑64 (MQ=255)
                                                                                         cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCCGCCACATCGCCGCCATGCCCGCCAAGAGGCCGGCCCAGACACCACAAAACC‑TCAGTTTTTCTacg‑cccc                                                      >  6:150357/1‑148 (MQ=255)
                                                                                          tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTAGCGGCCTGTACCGCGGATAGGCCGGCACACGAACCACAAAAAC‑AAAGGTTTTGTgcg‑ccccc                                                     >  3:203751/1‑147 (MQ=255)
                                                                                           gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCAAAGACACCACAAAACC‑TCAGTTTTTATCAG‑CCCGCt                                                    >  2:262864/1‑149 (MQ=255)
                                                                                             aaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCACTTTTTATCCG‑CCCCCTCa                                                  >  4:309773/1‑149 (MQ=255)
                                                                                                        tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TAAGTTTTTATACG‑CCCGCTCACGAAGCCCtt                                        >  4:89451/1‑148 (MQ=255)
                                                                                                          aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCa                                     <  4:265639/149‑1 (MQ=255)
                                                                                                                cttAGCCGACCCAAAGAAGGCTGCCCTCGGGCAGCGTTTTTTTTTTTGCCTGG‑GCCGGCCTCTTCCCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTAt                               <  2:232549/147‑1 (MQ=255)
                                                                                                                 gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGGCCTCTTCGTGGTCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTAtt                              <  7:249490/149‑1 (MQ=255)
                                                                                                                    gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                 <  2:372718/81‑1 (MQ=37)
                                                                                                                    gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                 >  1:372718/1‑81 (MQ=37)
                                                                                                                          cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCGCCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcg                      <  1:262864/149‑1 (MQ=255)
                                                                                                                             aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                  <  1:237204/149‑1 (MQ=255)
                                                                                                                              agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                 <  3:309773/149‑1 (MQ=255)
                                                                                                                               aaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca                >  2:111813/2‑149 (MQ=255)
                                                                                                                                 agGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac               >  7:73049/1‑148 (MQ=255)
                                                                                                                                     tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCCGCCGTGGCCGCGCACAGGCCGGCACAGACCCCCCAAAAAC‑ACAGGTTTTATACG‑CCCGCGACCGCCGCCCATGGAAGGGTTTGAACCGCGCAGCcgccacc           >  8:142090/1‑147 (MQ=255)
                                                                                                                                     tGCCCTCCTGCAGCC‑TTATTTATTTCTCCTGTGCCGGCCTCATAGCAGCCTTGCACTCGAAAACGCCGGCACAGACACCACAAAACC‑TAAATATTTCTCCG‑CCCGCTAACCCAGCCCTTTCAGGGAATTGAACCGCGCAAACACCACg           >  6:356207/1‑148 (MQ=255)
                                                                                                                                       cccTCGGGCAGCC‑TTTTTTATTTGGCCTGGGCCGGCCTATTCGGGGCCTTGCCCGCCAATAGGCCGGCACAGACACCACAAAACCGTCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa         <  1:314542/149‑1 (MQ=255)
                                                                                                                                       cccTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGa          <  2:231417/147‑1 (MQ=255)
                                                                                                                                         cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt      <  1:291707/149‑1 (MQ=255)
                                                                                                                                              ggctgcTTTTTTTTTTTGTCCTGTGCCGGCCTCTGCGGGGCGTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg  <  4:181700/144‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTG  >  NC_002947/4740657‑4740947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑