| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.0% | 106.4 / ‑3.0 | 33 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (17/15); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932490‑1932781 | aTGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑g < 1:6708/149‑1 (MQ=255) cgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑G‑cccgcg > 1:274952/1‑144 (MQ=255) cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAggg < 6:131970/147‑1 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGTAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTg > 1:273396/1‑148 (MQ=255) cGACAGCGGCTTTGCAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCGTTCGGGGGCATGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt < 8:219461/148‑1 (MQ=38) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 2:273396/148‑1 (MQ=255) cATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGCTA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAc < 1:102494/149‑1 (MQ=255) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa > 3:117657/1‑148 (MQ=255) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATc < 2:13112/149‑1 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 2:274952/149‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc > 6:120212/1‑137 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc < 5:120212/137‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 5:122033/1‑149 (MQ=255) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg > 5:118699/1‑149 (MQ=255) tttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTTGTCG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGGGATGTTCAGCTCCAGAAt > 1:118146/1‑149 (MQ=38) cgcgGGCAAGCCCGCTCCTACACAGTATATGCGTCCCCTG‑TA‑GGCGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGccc < 2:118146/148‑1 (MQ=39) cgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCCGCTCCAGAATCGAGCAGCTGCCCTgg > 7:367474/1‑149 (MQ=255) tgtcacgCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GTTGCGGGATT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGt < 4:233037/143‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 1:391408/1‑103 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 4:117657/149‑1 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 6:122033/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 7:272264/1‑149 (MQ=255) tACAAAGGATATGCGTCCCCTG‑TAGGGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 1:4374/1‑149 (MQ=255) tACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 8:181649/1‑149 (MQ=255) aCAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc > 2:83330/1‑117 (MQ=255) aCAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 1:83330/117‑1 (MQ=255) aaGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac < 7:181649/149‑1 (MQ=255) aGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 3:298979/1‑149 (MQ=255) gCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 6:81374/1‑149 (MQ=255) gTCGCCTG‑TA‑GGCGGGGTCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg < 3:273775/149‑1 (MQ=255) cTG‑TA‑GGAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 3:118680/1‑149 (MQ=255) ggAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc > 2:228078/1‑140 (MQ=255) ggAGCGGGCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 1:228078/140‑1 (MQ=255) ggCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAAACGAGCAGCTGCCCTGGTTTTCCAGCTCGATATTAAAATCATAGTTGACGATGTTGACATAtt > 6:333134/1‑149 (MQ=255) gCTT‑‑‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt > 6:310900/1‑149 (MQ=255) | ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTG‑TA‑GGAGCGGGCTT‑‑‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932490‑1932781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |