Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,586,035	+G	intergenic (+118/+97)	PP_4061 → / ← PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,586,033	1	.	G	100.0%	77.8 / NA	25	intergenic (+116/+99)	PP_4061/PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (4/21);  total (4/21)

GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑C‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAG  >  NC_002947/4585887‑4586113                                                                                                                                                       |                                                                                     gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg                                                                                    <  4:24654/149‑1 (MQ=255) gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg                                                                                    <  2:216030/149‑1 (MQ=255) gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg                                                                                    <  3:333732/149‑1 (MQ=255)       ccAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAc                                                                               >  5:388234/1‑149 (MQ=255)       ccAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAc                                                                               <  1:82253/149‑1 (MQ=255)       ccAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACAGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAc                                                                               <  5:239922/149‑1 (MQ=255)            aGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCt                                                                          <  3:141634/149‑1 (MQ=255)             gCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTc                                                                         <  4:156280/149‑1 (MQ=255)              ccAACGGTGAACGCGTAAGGGGAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGTTCGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCt                                                                          <  1:309184/149‑1 (MQ=255)                aCCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTa                                                                      <  4:117032/149‑1 (MQ=255)                    gTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCGAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAg                                                                  <  1:367465/149‑1 (MQ=39)                        aGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑c                                                            <  2:224307/149‑1 (MQ=255)                         ggCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑cc                                                           <  4:237868/149‑2 (MQ=255)                            ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAcc                                                                              <  3:142829/129‑1 (MQ=255)                            ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAcc                                                                              >  4:142829/1‑129 (MQ=255)                              aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑ccgtgc                                                       <  8:42988/148‑6 (MQ=39)                              aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑ccgtg                                                        <  8:218259/147‑5 (MQ=39)                                   tAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCt                                                 <  1:260215/149‑1 (MQ=39)                                              aCCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTCGAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCCCTCCTACCACGG‑ACC‑GTGCCGGCCTTGAATg‑cggc                                      >  1:75815/1‑149 (MQ=255)                                                           gCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAg                        <  4:133924/149‑1 (MQ=39)                                                                    cgcTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑G‑CAGCGCC‑GCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGt                <  6:388234/148‑1 (MQ=14)                                                                    cgcTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGt                <  7:12666/148‑1 (MQ=38)                                                                      ctgctCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTa             <  6:175594/149‑1 (MQ=38)                                                                            cATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTAccc          <  8:135314/146‑1 (MQ=38)                                                                            cATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcgc        <  8:22222/148‑1 (MQ=38)                                                                                  aaGGGT‑GTGGATGCCCGGGG‑‑GCTTGGCAAGGTGTAGATCTTGCATTGATGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCCGGGCC‑C‑GTGCCGGCCTTTAATG‑CGGCGCGGCCCCGGGTAGGAGCGGGTTTACCCGCGAagag  <  2:75815/148‑1 (MQ=21)                                                                                                                           cTTGCATTTTTCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAaga   <  6:111342/109‑1 (MQ=21)                                                                                                                           cTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAaga   >  5:111342/1‑109 (MQ=25)                                                                                                                                                       |                                                                                     GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTT‑GAGGATGACCGGGT‑‑GCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑C‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAG  >  NC_002947/4585887‑4586113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑