| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 307,866 | +C | intergenic (+881/‑988) | hslO → / → PP_0254 | molecular chaperone HslO/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 307,865 | 1 | . | C | 98.2% | 183.0 / NA | 54 | intergenic (+880/‑989) | hslO/PP_0254 | molecular chaperone HslO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (27/26); minor base A (1/0); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT > NC_002947/307718‑308008 | aaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGTCAGCCGAAAgg < 2:278138/149‑2 (MQ=255) ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGAt < 6:58726/147‑1 (MQ=255) cAGATCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACAAAAGCCGTTACCTGATCGGTg < 5:392015/148‑1 (MQ=255) cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg < 7:188117/148‑1 (MQ=255) aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGTTGACGAAAGCCGTTACCTGATCGGTgacga > 2:119894/1‑149 (MQ=255) ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGAcgacga < 2:67346/147‑1 (MQ=255) cggctgccgATGTACTGCCTATGCACTGCGCGGCCAAAATCGGCGAAGAAGGCGATGTTACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgg < 7:278634/149‑1 (MQ=255) ctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGTTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGc < 1:119894/149‑1 (MQ=255) cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg > 6:370668/1‑149 (MQ=255) cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg < 1:197008/149‑1 (MQ=255) cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg < 5:370668/149‑1 (MQ=255) gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGt < 8:370844/115‑1 (MQ=255) cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc < 8:4367/149‑1 (MQ=255) cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc < 3:196542/149‑1 (MQ=255) tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTAGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCa > 1:273875/1‑148 (MQ=255) gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa > 7:88151/1‑149 (MQ=255) gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa > 3:259611/1‑149 (MQ=255) cACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt > 8:338203/1‑149 (MQ=255) cACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt > 1:151276/1‑149 (MQ=255) aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCATTGTCTTCAACATg < 5:3206/149‑1 (MQ=255) tGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATgg > 5:392830/1‑148 (MQ=255) ggCCAAGATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTGTCTGCTGAGGAAAGCCGTTACCTGATCGGTGACGACGAGCTCGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcg < 5:375102/149‑1 (MQ=255) ccAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc < 6:392830/149‑1 (MQ=255) ggttgtggCGAAGAAGGAGCTGTGTCCTT‑GTTCTTCGGCCTTTCCGGCACTGGCAAGACTACACTTTCGGCTGACGAAAGCCGTTACCTTTTCGGGGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATTGAAGGCGgctg < 6:178407/143‑1 (MQ=255) ggCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc < 7:338203/149‑1 (MQ=255) gCGAAGAAGGCGATGTGACCAT‑GTTCTTCGGCCTGTCCGGAACTGGCAAGACTACCCTTTCGGCTAACGAAAGACGTTACATGATAGGTGACGAGCAGCACTGTTGTGGAGAATGCGTTCTCTTCAAAATGGAAGGctgctg > 5:178407/1‑142 (MQ=255) cGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGcc > 1:51687/1‑148 (MQ=255) aagaagGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg > 5:311514/1‑149 (MQ=255) tgtgACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAcc > 1:207853/1‑149 (MQ=255) gACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt > 6:295208/1‑149 (MQ=255) aCCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt < 5:295208/148‑1 (MQ=255) ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga < 2:207853/148‑1 (MQ=255) ccTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc > 3:171557/1‑149 (MQ=255) ccTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc < 2:273875/149‑1 (MQ=255) ccTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc > 5:145841/1‑149 (MQ=255) tCCGGCACTGGCAAGCCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg < 6:311514/148‑1 (MQ=255) ggCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCAt < 4:259611/149‑1 (MQ=255) tGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGCCGCCGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTCCGCCAAGTGCATCGACCGGTCCGAGAAGAACg > 3:366469/1‑134 (MQ=255) gCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGa < 2:51687/147‑1 (MQ=255) aaGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGc > 8:349855/1‑149 (MQ=255) gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCa > 1:89110/1‑149 (MQ=255) cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt > 5:182719/1‑148 (MQ=255) cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt > 5:182337/1‑148 (MQ=255) cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc < 2:151276/149‑1 (MQ=255) tACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt > 1:160119/1‑101 (MQ=255) tACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt < 2:160119/101‑1 (MQ=255) cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg > 6:103620/1‑149 (MQ=255) ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga > 7:306462/1‑116 (MQ=255) ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga < 8:306462/116‑1 (MQ=255) ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg > 2:218142/1‑148 (MQ=255) tttCGGCTGACGAAAGCCGTTACCTGATCGGTGACGAAGAGCACGGTTAAGGCGAAGCCGTTGTCATCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAACTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc > 7:246426/1‑149 (MQ=255) tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg > 3:23662/1‑149 (MQ=255) gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc > 7:26861/1‑80 (MQ=255) gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc < 8:26861/80‑1 (MQ=255) gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt > 2:6632/1‑149 (MQ=255) | AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTT‑GTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT > NC_002947/307718‑308008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |