Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,555,608	+GCC	intergenic (‑84/+87)	PP_4887 ← / ← PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5555608	1 (0.020)	48 (0.740) +GCC	45/274	0.5	98.0%	intergenic (‑84/+87)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer
	?	NC_002947	5555609 =	1 (0.020)					intergenic (‑85/+86)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

TGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555464‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTT  >  NC_002947/5555609‑5555754                                                                                                                                                  |||                                                                                                                                                     TGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTTTAGCGAAAGGGCCCCATAGCCGCCC                                                                                                                                                     >  2:131174/1‑149   TCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCC                                                                                                                                                     <  3:196458/147‑1           CAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCTGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTC                                                                                                                                           <  7:59596/149‑1             GGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCG                                                                                                                                         >  1:216623/1‑149                       GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                                >  3:126164/1‑148                        TCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCTGGCAATTTCCGCGTGTATGCAA                                                                                                                              <  4:331147/149‑1                          GGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                             >  2:217615/1‑148                           GCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACA                                                                                                                            >  3:118824/1‑148                                  CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GG                                                                                                                    >  3:350527/1‑148                                  CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GG                                                                                                                    <  4:350527/148‑1                                            TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                         <  4:126164/149‑1                                            TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATG                                                                                                          >  4:126885/1‑148                                              GGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCA                                                                                                        <  7:160044/148‑1                                                  GGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCT                                                                                                   <  6:130716/149‑1                                                    ACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCATAGCAGCCCcag                                                                                                 <  2:216623/149‑4                                                         AGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGA                                                                                            <  3:126885/149‑1                                                           CAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGTGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACA                                                                                          <  1:82211/149‑1                                                           CAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTAGGGGCTGCTATGCAGCCCTTTCGCGAC                                                                                           <  1:217615/149‑1                                                                   TTTGTCATGTCTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCCCATAGCCGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCC                                                                                  <  3:83171/149‑1                                                                     tggCATGTGCCCCCCACAGCATACCACCACCGCTCCGGTATGGGAGCGGCCTTGTGGCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCA                                                                                 <  1:131174/145‑1                                                                      GTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTAGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCAC                                                                                <  2:6569/148‑1                                                                         ATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTAGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCGTTCGCGACACAAGGCCCACAAAG                                                                            >  5:400578/1‑149                                                                          TGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCG                                                                                               >  8:224469/1‑129                                                                          TGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCG                                                                                               <  7:224469/129‑1                                                                              TCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTAGCGAACGGGCCGCCTAGCGGCCCCGGCAATTTCCGCGTGTATGCCACAAACCT‑GGGGCAGCTATGCAGCCCATTCGCCACACCAGGCCCACACAGGAACG                                                                       >  6:88157/1‑149                                                                                      AGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCT                                                               >  1:94403/1‑149                                                                                        CATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTAT                                                                                                           <  4:361017/103‑1                                                                                        CATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTAT                                                                                                           >  3:361017/1‑103                                                                                         ATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTGCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCCGCCCGTTCGCGACAC                                                                                         >  8:101003/1‑120                                                                                         ATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACAC                                                                                         <  7:101003/120‑1                                                                                            GCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCTACACAAGGCCCACAAAGGACCGCGCAGGC                                                                <  8:103462/142‑1                                                                                            GCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGC                                                                >  7:103462/1‑142                                                                                             CAACCCCGCTCAGGGGTGGGAGCGGCCTTGTGACGCTAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAG                                                                            <  3:179170/129‑1                                                                                              AACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCTAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑C                                                       <  6:400578/148‑1                                                                                                 ACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTGCGCCTGTATGCAACAAACCT‑GGGGCTGCTGTGCAGCCCTTTCGCGCCACAAGGCCCACAAAGGACCGCGCCGGCTTCAGGC‑CCGG                                                    >  5:345397/1‑148                                                                                                        AGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGG                                              <  3:158648/147‑1                                                                                                                GAGCGGCATTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGGTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCACGCTTCAGGC‑CCGGAACTGGCCCAGGCTG                                     <  1:223758/148‑1                                                                                                                  GCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCG                                  <  3:269779/149‑1                                                                                                                      CCTTGTGTCGCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCA                              <  7:78844/149‑1                                                                                                                               GCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCG                     >  2:307516/1‑149                                                                                                                               GCGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCATTTAGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAAGC‑CCGCAACTGGCCCACGATGGCGCGCAGCTGAGCCG                     >  4:75567/1‑149                                                                                                                                CGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGCACTGGCCCAGGCTGGCGCGCAGCGGCGCCGC                    >  2:385170/1‑149                                                                                                                                CGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                    <  6:98283/149‑1                                                                                                                                CGAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                    >  2:101832/1‑149                                                                                                                                       GCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACCAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA             >  6:51420/1‑149                                                                                                                                         CGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATC           <  4:118824/149‑1                                                                                                                                          GCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC          <  2:94403/149‑1                                                                                                                                                CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACC    >  5:167227/1‑149                                                                                                                                                  cCCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTT  <  1:385170/148‑1                                                                                                                                                   CCCCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTTCTATGCATCCCTTTAGCGACACAAGGCCCACAAACGACCGCGCAGGCTTCAGGCTCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTT  <  1:101832/149‑1                                                                                                                                                  |||                                                                                                                                                     TGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555464‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGC‑CCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTT  >  NC_002947/5555609‑5555754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.