Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	0 (0.000)	25 (0.390) +CGGG	25/272	2.2	100%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	0 (0.000)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

ACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681271‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCC  >  NC_002947/5681416‑5681553                                                                                                                                                    ||||                                                                                                                                            ACCTTAAGACATGCAATCCCCGAGCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGAGTGCTTCGTGCTTTGTGGGAGCGGCGTTGTGTCGCTAAAGGGGCGCGAAGGGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGG                                                                                                                                              <  8:290880/148‑1     TAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTG                                                                                                                                         <  4:118253/149‑1            TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCA                                                                                                                                  >  7:63811/1‑149               AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGTGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCC                                                                                                                               >  3:51713/1‑149                    CAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATC                                                                                                                          <  8:281792/149‑1                     AGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTAGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTAATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCCGCCCTATag                                                                                                                         >  5:349469/1‑147                         CTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGA                                                                                                                      >  2:57918/1‑148                            CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTGGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGGAGCGCACCCCGATAGCcaaac                                                                                                                   >  3:216045/1‑143                               AGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGTGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAG                                                                                                                <  4:51713/148‑1                                 GTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                              <  8:63811/148‑1                                    GGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGC                                                                                                           <  2:73023/148‑1                                     GCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCC                                                                                                                               <  6:301896/127‑1                                     GCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCC                                                                                                                               >  5:301896/1‑127                                                                 TTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCCCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCC                                                                              >  8:263954/1‑148                                                                               GAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGTGGCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAAC                                                                  >  1:313721/1‑148                                                                                aggGGCCTTGTGTCGCGAATGGGGCGCGACGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAG                                                              <  2:313721/146‑1                                                                                              GCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑A                                                >  4:112065/1‑148                                                                                               CGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATC                                              >  7:230034/1‑149                                                                                                     GGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTC                                        <  7:263954/149‑1                                                                                                                     CAGGTTTTCATTGCCATTGAATGACCC‑‑GCCGGGGCTGCTCCGCAGCCCTATCGCCACAAACGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCC                        <  4:216045/149‑1                                                                                                                       GGCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAA                      <  3:112065/149‑1                                                                                                                        GCTTTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAAT                     <  8:230034/149‑1                                                                                                                           TTCATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAATGC                   >  2:277601/1‑148                                                                                                                             CATTGCCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAATGCGA                 <  1:277601/148‑1                                                                                                                                  CCACTGCATGAACC‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGAATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAG             <  1:57918/148‑1                                                                                                                                           GAACC‑‑GCCGGGGCTGCTGCGCAGCCCAATAGCGACACAAGGCCGCTCCCACTCCAGACCACCACGCCCAGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCC  >  2:140642/1‑149                                                                                                                                                    ||||                                                                                                                                            ACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑GC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681271‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAG‑ATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCC  >  NC_002947/5681416‑5681553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.