Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG98.0% 159.6 / NA 51intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (23/27);  minor base C (1/0);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740677‑4740952
                                                                                                                                           |                                                                                                                                          
tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGACCGCAAGACGGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                  >  8:249490/1‑149 (MQ=255)
 ccAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                 >  2:374440/1‑149 (MQ=255)
 ccAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGT‑AGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTACTGTGCCGGc                                                                                                                                >  1:232549/1‑149 (MQ=255)
  cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                <  6:59706/149‑1 (MQ=255)
  cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                <  1:41226/149‑1 (MQ=255)
      ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                            <  2:357637/149‑1 (MQ=255)
           cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                  <  8:359963/138‑1 (MQ=255)
           cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                  >  7:359963/1‑138 (MQ=255)
                tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                  >  4:102043/1‑149 (MQ=255)
                      gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                            >  6:139362/1‑149 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                          <  1:374440/148‑1 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                          <  5:278010/148‑1 (MQ=255)
                          gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                        <  6:155371/149‑1 (MQ=255)
                           cgcCAGCTGTACTCCTGCCAGCAGAAGGGCGACATCGTTGCCCTGTAAGACCGCATCTAAGCTGCACTAGCCGAAGGCTAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCGTCTTCGCGGCCTTGCCCGCGAaga                                                                                                       <  5:304301/149‑1 (MQ=255)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCGCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                      <  5:334652/149‑1 (MQ=38)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                       <  5:146928/149‑1 (MQ=38)
                                    tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca                                                                                               >  2:237204/1‑148 (MQ=255)
                                    tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTCAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAATAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcac                                                                                              <  6:196617/149‑1 (MQ=255)
                                      cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAAGAGGCCGGcaca                                                                                             <  6:204310/148‑1 (MQ=37)
                                       acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGGCGTGGCCGCGAAGAGggcggcgccgg                                                                                           >  1:381326/1‑144 (MQ=255)
                                             gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcacca                                                                                      >  3:265639/1‑148 (MQ=255)
                                              aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcaccac                                                                                     >  2:291707/1‑148 (MQ=255)
                                                     gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑t                                                                             >  1:231417/1‑148 (MQ=255)
                                                          aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGttt                                                                       <  5:139362/149‑1 (MQ=255)
                                                               gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGGTTTtctc                                                                  >  3:316504/1‑149 (MQ=255)
                                                                 tGCCCTGAAAGACCGAAAGTAACCTGCAGTACCCTAACCAAACACGGCTGCCCGCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCTCTTCGCGCCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTcc                                                                 <  2:249522/148‑1 (MQ=255)
                                                                   cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAACC‑TCAGATTTTCTacg‑cc                                                             >  3:181700/1‑149 (MQ=255)
                                                                     cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCCGCCACATCGCCGCCATGCCCGCCAAGAGGCCGGCCCAGACACCACAAAACC‑TCAGTTTTTCTacg‑cccc                                                           >  6:150357/1‑148 (MQ=255)
                                                                      tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTAGCGGCCTGTACCGCGGATAGGCCGGCACACGAACCACAAAAAC‑AAAGGTTTTGTgcg‑ccccc                                                          >  3:203751/1‑147 (MQ=255)
                                                                       gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCAAAGACACCACAAAACC‑TCAGTTTTTATCAG‑CCCGCt                                                         >  2:262864/1‑149 (MQ=255)
                                                                         aaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCACTTTTTATCCG‑CCCCCTCa                                                       >  4:309773/1‑149 (MQ=255)
                                                                                    tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TAAGTTTTTATACG‑CCCGCTCACGAAGCCCtt                                             >  4:89451/1‑148 (MQ=255)
                                                                                      aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCa                                          <  4:265639/149‑1 (MQ=255)
                                                                                            cttAGCCGACCCAAAGAAGGCTGCCCTCGGGCAGCGTTTTTTTTTTTGCCTGG‑GCCGGCCTCTTCCCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTAt                                    <  2:232549/147‑1 (MQ=255)
                                                                                             gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGGCCTCTTCGTGGTCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTAtt                                   <  7:249490/149‑1 (MQ=255)
                                                                                                gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                      >  1:372718/1‑81 (MQ=37)
                                                                                                gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                      <  2:372718/81‑1 (MQ=37)
                                                                                                      cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCGCCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcg                           <  1:262864/149‑1 (MQ=255)
                                                                                                         aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                       <  1:237204/149‑1 (MQ=255)
                                                                                                          agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                      <  3:309773/149‑1 (MQ=255)
                                                                                                           aaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca                     >  2:111813/2‑149 (MQ=255)
                                                                                                             agGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                    >  7:73049/1‑148 (MQ=255)
                                                                                                                 tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCCGCCGTGGCCGCGCACAGGCCGGCACAGACCCCCCAAAAAC‑ACAGGTTTTATACG‑CCCGCGACCGCCGCCCATGGAAGGGTTTGAACCGCGCAGCcgccacc                >  8:142090/1‑147 (MQ=255)
                                                                                                                 tGCCCTCCTGCAGCC‑TTATTTATTTCTCCTGTGCCGGCCTCATAGCAGCCTTGCACTCGAAAACGCCGGCACAGACACCACAAAACC‑TAAATATTTCTCCG‑CCCGCTAACCCAGCCCTTTCAGGGAATTGAACCGCGCAAACACCACg                >  6:356207/1‑148 (MQ=255)
                                                                                                                   cccTCGGGCAGCC‑TTTTTTATTTGGCCTGGGCCGGCCTATTCGGGGCCTTGCCCGCCAATAGGCCGGCACAGACACCACAAAACCGTCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa              <  1:314542/149‑1 (MQ=255)
                                                                                                                   cccTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGa               <  2:231417/147‑1 (MQ=255)
                                                                                                                     cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt           <  1:291707/149‑1 (MQ=255)
                                                                                                                          ggctgcTTTTTTTTTTTGTCCTGTGCCGGCCTCTGCGGGGCGTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg       <  4:181700/144‑1 (MQ=255)
                                                                                                                            ctgccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGc     <  5:356207/144‑1 (MQ=255)
                                                                                                                               ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGGGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACCAACTTGTTGGccac  >  5:107771/3‑149 (MQ=255)
                                                                                                                               ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcc    <  1:111813/145‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACC‑TCAGTTTTTCTCCG‑CCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740677‑4740952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑