BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,984,832	G→A	D96D (GAC→GAT)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,984,832	0	G	A	91.4%	1783.7 / 139.3	844	D96D (GAC→GAT)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (37/35); new base A (395/372); total (432/412)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 844 total aligned reads displayed.

AAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCC  >  NC_002947/5984689‑5984979

   gTGACCGATGCCTCGGGCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGtt                                                                                                                                                >  3:151492/1‑149 (MQ=255)

                                                                 gAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCttt                                                                                 >  1:100901/1‑147 (MQ=255)

                                                                    ggCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCt                                                                            >  5:52153/1‑149 (MQ=255)

                                                                                         cacaCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCg                                                       <  7:5070/149‑1 (MQ=255)

                                                                                                                      cggtcgCCTTCACTGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                 <  4:207401/137‑1 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  6:165707/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  4:57807/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  7:340089/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  3:186519/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  2:31667/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  2:165524/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                   <  3:336484/142‑1 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAAGGTCgg                     >  4:293125/8‑147 (MQ=11)

                                                                                                                             cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGTCGCATCCGGGAACATCTCATTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                   <  6:224853/142‑1 (MQ=11)

                                                                                                                              ttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                   >  3:313878/7‑132 (MQ=11)

                                                                                                                               tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                 >  3:221466/6‑149 (MQ=11)

                                                                                                                               tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                 >  1:32310/6‑149 (MQ=11)

                                                                                                                                caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCgg                                                      <  8:169246/107‑1 (MQ=11)

                                                                                                                                caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                 <  4:359789/144‑1 (MQ=11)

                                                                                                                                caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                >  5:253215/5‑149 (MQ=11)

                                                                                                                                 cacgCCTTGACGAAAACCTCGGTGGCCCAGGCGTTTCTCTGGCCCTGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGCACATCTCTTTCCTCTCGGGCACCATCGGGGCAATCGGGCCTTTCTCGAAGCTGGGGGGGTGGATGGTCGGCAGGc                <  2:74938/145‑1 (MQ=9)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCGAGGCGTTGCTCTGGCCAGGGGGGGCAA‑T‑GTA‑CGGCGGATCCGGGAAGATCTCTTTCTGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCTGGc                <  2:137041/145‑1 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTc                                                        <  6:134158/105‑1 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                   >  4:308731/4‑129 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                <  6:383481/145‑1 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                <  4:165708/145‑1 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                >  8:29254/4‑148 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                >  4:331539/4‑148 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                <  1:191749/145‑1 (MQ=11)

                                                                                                                                 accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                <  3:206844/145‑1 (MQ=11)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                         >  1:4450/2‑121 (MQ=11)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                              >  4:96165/2‑132 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                          >  3:140992/2‑136 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaa             <  6:72052/148‑1 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaa             <  8:331812/148‑1 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  5:347989/2‑148 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  1:384872/2‑148 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  2:20538/2‑148 (MQ=255)

                                                                                                                                   cgCCTTGACGAAATCCTCGTTGTCCCAGGCGATGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaa             <  8:25096/148‑1 (MQ=14)

                                                                                                                                    gCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaa             <  2:100901/148‑1 (MQ=255)

                                                                                                                                    gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGTTCGGCAGGCCaaa            <  5:125895/149‑1 (MQ=14)

                                                                                                                                    gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa            <  8:3784/149‑1 (MQ=255)

                                                                                                                                    gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa            <  5:176404/149‑1 (MQ=255)

                                                                                                                                    gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa            <  1:366569/149‑1 (MQ=255)

                                                                                                                                    gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  3:380488/1‑147 (MQ=255)

                                                                                                                                    gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  6:34549/1‑147 (MQ=255)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                 >  2:257790/1‑127 (MQ=255)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  7:147453/1‑146 (MQ=255)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  8:139795/1‑146 (MQ=255)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  1:72992/1‑146 (MQ=255)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg           <  1:210978/149‑1 (MQ=255)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCAGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  7:213365/1‑146 (MQ=11)

                                                                                                                                     ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCGCGGGCACCCGCCGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa              >  4:109856/1‑146 (MQ=14)

                                                                                                                                      cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg           >  8:181266/1‑148 (MQ=255)

                                                                                                                                      cTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg           >  7:191366/1‑148 (MQ=255)

                                                                                                                                       ttGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa            >  6:333611/1‑146 (MQ=255)

                                                                                                                                       ttGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         >  1:305404/1‑149 (MQ=255)

                                                                                                                                       ttGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         >  5:69818/1‑149 (MQ=255)

                                                                                                                                       ttGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         >  5:89666/1‑149 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGGAA‑T‑GTA‑CGGCGCATCCGTGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         <  2:187583/147‑1 (MQ=14)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                <  5:388644/108‑1 (MQ=14)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                              <  2:55030/110‑1 (MQ=14)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                >  7:326316/1‑124 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                >  1:157063/1‑124 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                     >  6:193096/1‑135 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         <  7:29254/147‑1 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         <  3:4216/147‑1 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa         <  8:229380/147‑1 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc        <  5:310528/148‑1 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc        <  3:254415/148‑1 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc        <  2:287004/148‑1 (MQ=255)

                                                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGCAACTCCCACCGGCGCATCCGGGAAGATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa            <  1:127405/147‑1 (MQ=14)

                                                                                                                                          aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                >  3:290432/1‑123 (MQ=255)

                                                                                                                                          aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt      >  8:66424/1‑149 (MQ=255)

                                                                                                                                          aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt      >  2:218020/1‑149 (MQ=255)

                                                                                                                                          aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt      >  2:379695/1‑149 (MQ=255)

                                                                                                                                          aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt      <  7:99990/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCATCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  5:366579/149‑1 (MQ=14)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTTCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  6:293073/149‑1 (MQ=14)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  6:26618/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  1:270083/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  3:65008/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  4:229497/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  7:239513/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  5:203663/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  7:255553/149‑1 (MQ=255)

                                                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAACGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  2:93237/149‑1 (MQ=14)

                                                                                                                                           cGAAATCCTCGGTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     <  4:280621/149‑1 (MQ=14)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                              <  7:178165/107‑1 (MQ=14)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                         >  5:288113/1‑112 (MQ=14)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                <  8:20470/137‑1 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    >  7:36645/1‑149 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    <  4:382278/149‑1 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    >  2:43862/1‑149 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    >  4:138158/1‑149 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    >  2:345312/1‑149 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    >  6:98087/1‑149 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    <  1:233352/149‑1 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg    >  6:454/1‑149 (MQ=255)

                                                                                                                                            gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg     >  3:84466/1‑148 (MQ=255)

AAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAA‑T‑GTA‑CGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCC  >  NC_002947/5984689‑5984979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`