Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,984,865 A→G R85R (CGT→CGC)  ycaC‑II ← putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,984,8650AG96.9% 5542.4 / 85.3 1950R85R (CGT→CGC) ycaC‑IIputative hydrolase
Reads supporting (aligned to +/- strand):  ref base A (28/32);  new base G (997/887);  total (1027/923)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-01

Only 100 of 1951 total aligned reads displayed.

ACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGA  >  NC_002947/5984717‑5985010
                                                                                                                                                     |                                                                                                                                                          
                                     gAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCttt                                                                                                                         >  1:100901/1‑147 (MQ=255)
                                     gAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCttt                                                                                                                         >  3:72638/1‑147 (MQ=255)
                                                        gTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGc                                                                                                     >  7:25685/1‑148 (MQ=255)
                                                                                  cttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCtggtgg                                                                 <  4:148643/149‑1 (MQ=255)
                                                                                                 cttcaccgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCgg                                                    >  2:642/8‑147 (MQ=11)
                                                                                                 cttcaccgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCgg                                                    >  6:165707/8‑147 (MQ=11)
                                                                                                 cttcaccgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCgg                                                    >  6:391299/8‑147 (MQ=11)
                                                                                                 cttcaccgCCTTGACGAAATCCTCGTTGA‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCgg                                                    >  1:221503/8‑147 (MQ=11)
                                                                                                 cTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGAGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCgg                                                    >  2:189024/1‑147 (MQ=255)
                                                                                                  ttcaccgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAg                                                 >  3:342201/7‑149 (MQ=11)
                                                                                                    caccgCCTTGACGAAATCCTCGTTGTGCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑Gaa                                                                         >  1:204595/5‑124 (MQ=11)
                                                                                                    caccgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGc                                               >  3:169896/5‑149 (MQ=11)
                                                                                                     accgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGc                                               <  7:183862/145‑1 (MQ=11)
                                                                                                     accgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGc                                               <  5:45713/145‑1 (MQ=11)
                                                                                                     accgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGCCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                              <  5:334127/146‑1 (MQ=11)
                                                                                                      ccgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                              >  4:4391/3‑148 (MQ=255)
                                                                                                       cgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑Cgg                                                                                         <  1:175090/107‑1 (MQ=11)
                                                                                                       cgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAg                                                             <  6:105534/131‑1 (MQ=255)
                                                                                                       cgCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                             >  7:320509/2‑148 (MQ=255)
                                                                                                        gCCTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                           <  4:285459/149‑1 (MQ=255)
                                                                                                         ccTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCtgg                                                                    <  6:369383/123‑1 (MQ=255)
                                                                                                         ccTTGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg                                          <  6:255329/149‑1 (MQ=255)
                                                                                                           ttGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCa                                                  >  4:188038/1‑139 (MQ=255)
                                                                                                           ttGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGa                                         >  8:102534/1‑148 (MQ=255)
                                                                                                            tGACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                       >  2:209524/1‑149 (MQ=255)
                                                                                                             gACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGa                                                           <  5:199868/128‑1 (MQ=255)
                                                                                                             gACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                       <  1:68428/148‑1 (MQ=255)
                                                                                                             gACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                       <  6:52274/148‑1 (MQ=255)
                                                                                                             gACGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACt                                      >  7:167130/1‑149 (MQ=255)
                                                                                                              aCGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                     <  8:206703/149‑1 (MQ=255)
                                                                                                              aCGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                     >  6:142089/1‑149 (MQ=255)
                                                                                                               cGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGTGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                    >  5:236670/1‑149 (MQ=14)
                                                                                                               cGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTT‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                    <  6:293073/149‑1 (MQ=14)
                                                                                                               cGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                    >  2:127614/1‑149 (MQ=255)
                                                                                                               cGAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                    <  1:376700/149‑1 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGc                                               >  7:20470/1‑137 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                   >  4:307541/1‑149 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                   >  8:258742/1‑149 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                   >  3:224971/1‑149 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                   >  6:342332/1‑149 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                   >  2:43862/1‑149 (MQ=255)
                                                                                                                gAAATCCTCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCAGGGAACATCTCTTTAAGCTCGGGCACAAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                   >  5:361215/1‑149 (MQ=14)
                                                                                                                 aaatcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                  <  7:234136/143‑1 (MQ=255)
                                                                                                                 aaatcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                  <  1:305965/143‑1 (MQ=255)
                                                                                                                   atcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGGAATGGACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGCACTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGcc                                 <  1:97037/145‑1 (MQ=14)
                                                                                                                   atcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                  >  2:259182/5‑147 (MQ=255)
                                                                                                                    tcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGa                                         <  8:164510/136‑1 (MQ=255)
                                                                                                                    tcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                               <  2:22756/146‑1 (MQ=255)
                                                                                                                    tcctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                               >  3:315890/4‑149 (MQ=255)
                                                                                                                     cctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAgg                              >  1:341498/3‑149 (MQ=255)
                                                                                                                      ctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGt                             >  6:218671/2‑149 (MQ=255)
                                                                                                                      ctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGt                             >  1:48316/2‑149 (MQ=255)
                                                                                                                      ctCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGAGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGt                             >  7:288989/2‑149 (MQ=255)
                                                                                                                      ctCGGTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAgg                              <  8:33678/147‑1 (MQ=255)
                                                                                                                       tCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                            >  2:287253/1‑149 (MQ=255)
                                                                                                                       tCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                            >  4:32388/1‑149 (MQ=255)
                                                                                                                       tCGTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                            >  8:363879/1‑149 (MQ=255)
                                                                                                                         ggtGT‑CCCAGGCGGTGTTCTTGCCCGGGCGGGCAATGTACGGCGCTTCCGTGAACATCTCTTTCCGCGCGGGCCACAGCGTGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGGGAGGATGGTCGTCCGGCCAAAGAACTTGGCCAGGCCGc                          <  5:144824/147‑1 (MQ=17)
                                                                                                                         gTTGT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGc                          <  5:384037/149‑1 (MQ=255)
                                                                                                                            gT‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGccc                        <  2:310670/148‑1 (MQ=255)
                                                                                                                             t‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGccc                        >  8:62226/1‑147 (MQ=255)
                                                                                                                             t‑CCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGccc                        >  3:297529/1‑147 (MQ=255)
                                                                                                                               cccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                     >  4:12809/1‑149 (MQ=255)
                                                                                                                                ccAGGCTTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                     <  7:346123/148‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTc                                                                              <  4:76708/93‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGc                                               <  8:301363/122‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                    >  1:25436/1‑149 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                    <  2:88105/149‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                    <  8:23386/149‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                     <  8:179144/148‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                     <  4:213921/148‑1 (MQ=255)
                                                                                                                                ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                     <  3:97277/148‑1 (MQ=255)
                                                                                                                                  aGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGTCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                  <  2:341498/149‑1 (MQ=255)
                                                                                                                                   ggCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                   >  6:31953/1‑147 (MQ=255)
                                                                                                                                   ggCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCa                 <  4:247209/149‑1 (MQ=255)
                                                                                                                                    gCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                               <  3:206282/134‑1 (MQ=255)
                                                                                                                                    gCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAg                <  5:175323/149‑1 (MQ=255)
                                                                                                                                    gCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAg                >  3:147205/1‑149 (MQ=255)
                                                                                                                                     cGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc               <  7:44319/149‑1 (MQ=255)
                                                                                                                                      gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCa                                                  <  3:258258/113‑1 (MQ=255)
                                                                                                                                      gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAgg                                                <  5:311189/115‑1 (MQ=255)
                                                                                                                                      gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa              >  5:283618/1‑149 (MQ=255)
                                                                                                                                      gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa              >  7:134451/1‑149 (MQ=255)
                                                                                                                                      gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa              >  4:374563/1‑149 (MQ=255)
                                                                                                                                       ttGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTATANGGGGGC‑ATGGTATCGAGAAGAAGGTGGTGATGATGGTAGGCAGGCCAAAAAAATTGGCCAAGACGCCCaac                     >  1:126905/1‑148 (MQ=9)
                                                                                                                                       ttGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAgg                                                <  3:381389/114‑1 (MQ=255)
                                                                                                                                        tGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGcc                         >  7:65802/1‑136 (MQ=255)
                                                                                                                                         gATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACg            <  5:1011/148‑1 (MQ=255)
                                                                                                                                          aTCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa              >  2:164765/1‑145 (MQ=255)
                                                                                                                                          aTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgt           <  2:366820/148‑1 (MQ=255)
                                                                                                                                          aTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgt           <  5:75804/148‑1 (MQ=255)
                                                                                                                                             tGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgtt       >  7:101601/1‑149 (MQ=255)
                                                                                                                                              ggCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg         <  1:282469/146‑1 (MQ=255)
                                                                                                                                              ggCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc      >  6:73841/1‑149 (MQ=255)
                                                                                                                                               gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GCAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt     >  4:34738/1‑149 (MQ=255)
                                                                                                                                               gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAATAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt     >  6:194506/1‑149 (MQ=255)
                                                                                                                                               gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg         <  3:124533/145‑1 (MQ=255)
                                                                                                                                               gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgtt          <  8:131097/144‑1 (MQ=255)
                                                                                                                                               gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc      <  3:49837/148‑1 (MQ=255)
                                                                                                                                               gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt     >  1:154599/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                          
ACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGT‑CCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATT‑‑‑‑‑CGGGC‑CTTG‑CTC‑‑GAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGA  >  NC_002947/5984717‑5985010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑