BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,990,451	T→C	E72E (GAA→GAG)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,990,451	0	T	C	97.6%	3644.2 / 52.8	1777	E72E (GAA→GAG)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base T (19/24); new base C (889/833); total (917/860)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 1778 total aligned reads displayed.

GCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCA  >  NC_002947/5990305‑5990598

 ccACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcg                                                                                                                                                  >  1:378891/1‑148 (MQ=255)

 ccACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcg                                                                                                                                                  <  6:324242/149‑2 (MQ=255)

  cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcgg                                                                                                                                                 >  8:319136/1‑147 (MQ=255)

  cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcgg                                                                                                                                                 >  7:194963/1‑147 (MQ=255)

  cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTcgg                                                                                                                                                 >  6:291322/1‑147 (MQ=255)

   aCGCAAACCTCGGTCACACCCCCTGCACAGATCAGCTGCTTCTTACCGGTC‑GCCTTCACCCCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                                                                                                <  4:263885/149‑1 (MQ=18)

   aCGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                                                                                                <  4:96266/149‑1 (MQ=18)

   aCGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                                                                                                >  6:77683/1‑149 (MQ=18)

    cGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                                                                                                >  4:4216/1‑148 (MQ=18)

    cGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGc                                                                                                                                               <  3:329184/149‑1 (MQ=18)

     gCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCa                                                                                                                                              >  8:296322/1‑149 (MQ=18)

     gCAAACCTCGGTCACAACACCTGCAATGAACAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCa                                                                                                                                              >  1:230258/1‑149 (MQ=18)

      cAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCAc                                                                                                                                             <  3:162721/149‑1 (MQ=18)

       aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCAc                                                                                                                                             >  2:291607/1‑148 (MQ=18)

       aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCATTCAGCTCGGGCAc                                                                                                                                             >  2:86090/1‑148 (MQ=18)

        aaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCa                                                                                                                                           <  8:162890/149‑1 (MQ=18)

         aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTCTCAGCTCGGGCACCAg                                                                                                                                          <  5:122066/149‑1 (MQ=18)

            tCGGTCACAACAGCTGGAATGATCATCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCgg                                                                                                                                       <  8:340686/149‑1 (MQ=17)

            tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCgg                                                                                                                                       <  6:35626/149‑1 (MQ=17)

            tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTA‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTAACA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCATTCAGCTCGGGCagctcgg                                                                                                                                        >  5:51802/1‑142 (MQ=17)

              ggTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCggg                                                                                                                                      <  3:299389/148‑1 (MQ=17)

              ggTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCgg                                                                                                                                       <  1:357324/147‑1 (MQ=17)

              ggTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGc                                                                                                                                     <  4:134480/149‑1 (MQ=17)

               gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCAA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACTGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGc                                                                                                                                     >  6:226818/1‑148 (MQ=17)

                tCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGcca                                                                                                                                   >  1:9069/1‑148 (MQ=17)

                tCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGcca                                                                                                                                   >  8:42801/1‑148 (MQ=17)

                tCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGcca                                                                                                                                   >  3:111587/1‑148 (MQ=17)

                 cacaACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGccat                                                                                                                                  >  1:187583/1‑147 (MQ=17)

                  acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAtt                                                                                                                                 >  8:68300/1‑149 (MQ=17)

                  acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAtt                                                                                                                                 >  1:208467/1‑149 (MQ=17)

                  acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGACAGGGCGGGCAATGTACTGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAtt                                                                                                                                 >  7:92099/1‑149 (MQ=17)

                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttcg                                                                                                                               <  4:361761/149‑3 (MQ=17)

                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttcg                                                                                                                               <  3:26838/149‑3 (MQ=17)

                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttcg                                                                                                                               <  6:198525/149‑3 (MQ=17)

                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttcg                                                                                                                               <  7:366678/149‑3 (MQ=17)

                     acacCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTcgg                                                                                                                              <  3:139134/149‑1 (MQ=17)

                     acacCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTcgg                                                                                                                              >  4:109567/1‑149 (MQ=17)

                       acCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTATGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGc                                                                                                                            <  6:154085/149‑1 (MQ=17)

                       acCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGc                                                                                                                            >  2:388730/1‑149 (MQ=17)

                        ccTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           <  8:194963/149‑1 (MQ=17)

                         cTGCAATGATCATGTGCTTCTTTCCGGTC‑GCCTTCACCGCCTTGACGAAATTCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGTGCAATGTACGGCGCATCCGTGAACATCTCTTTCAGCTCGGGCACCAGAGGGCCATTCGGGCCt                                                                                                                          <  4:333192/149‑1 (MQ=14)

                         cTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                                                          >  5:315243/1‑149 (MQ=17)

                         cTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                                                          >  8:265784/1‑149 (MQ=17)

                         cTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                                                          <  2:195891/149‑1 (MQ=17)

                           gCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  8:133129/1‑149 (MQ=14)

                           gCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  5:395855/1‑149 (MQ=14)

                           gCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  5:72052/1‑149 (MQ=14)

                           gCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  3:55101/1‑149 (MQ=14)

                           gCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATACTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGAGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGc                                                                                                                                     >  7:246962/1‑136 (MQ=17)

                            cAATGATCAGCTGCTTCTTGCCGGTCGGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  1:5141/1‑149 (MQ=14)

                            cAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        >  1:309406/1‑149 (MQ=14)

                            cAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCTGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                                                                       >  5:155663/1‑149 (MQ=14)

                            cAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCtt                                                                                                                         <  5:198649/147‑1 (MQ=14)

                            cAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                                                        <  1:167971/148‑1 (MQ=14)

                            cAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                                                                       <  7:326103/149‑1 (MQ=14)

                            cAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                                                                       >  5:341501/1‑149 (MQ=14)

                             aaTGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGGGGGCCATTCGGGCCTTGCt                                                                                                                      <  6:175153/149‑1 (MQ=14)

                             aaTGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGc                                                                                                                                     <  3:90876/134‑1 (MQ=17)

                              aTGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGTTTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                     <  1:102248/149‑1 (MQ=14)

                               tGATCAGCTGCTTCTTGACGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                     >  4:296122/1‑148 (MQ=14)

                                gATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      >  2:60406/1‑146 (MQ=14)

                                gATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCg                                                                                                                    >  5:180296/1‑148 (MQ=14)

                                 aTCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGc                                                                                                                                               >  8:233955/1‑120 (MQ=17)

                                 aTCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  1:7447/149‑1 (MQ=14)

                                  tCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                  <  4:156530/148‑1 (MQ=14)

                                  tCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                 >  5:281431/1‑149 (MQ=14)

                                  tCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                 <  7:273897/149‑1 (MQ=14)

                                   cAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGc                                                                                                                >  2:233352/1‑149 (MQ=14)

                                   cAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGc                                                                                                                >  2:143311/1‑149 (MQ=14)

                                     gctgctTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      >  3:234177/1‑141 (MQ=14)

                                     gctgctTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTAGGGCACCAGCGGACCATTCGGGCCTTGCTCGAAGCtg                                                                                                              >  2:337320/1‑149 (MQ=14)

                                      ctgctTCTTGCCGGTC‑GCCTTCGCCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                             >  7:58124/1‑149 (MQ=14)

                                      ctgctTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                             >  5:223294/1‑149 (MQ=14)

                                        gctTCTTGCCGGTC‑GCCTTCACCGCCTTGAGGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                           >  2:315388/1‑149 (MQ=14)

                                        gctTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCggg                                                                                                                                      >  6:123156/1‑122 (MQ=17)

                                        gctTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                           >  2:357882/1‑149 (MQ=14)

                                        gctTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg                                                                                                           >  2:11407/1‑149 (MQ=255)

                                         cttcttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      <  4:204857/137‑1 (MQ=14)

                                         cttcttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                           <  7:115691/148‑1 (MQ=14)

                                          ttcttGCCGGTC‑GCCTTCACCGCCTTGACGTAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                                                           >  1:33178/1‑131 (MQ=14)

                                          ttcttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                         >  5:91426/1‑149 (MQ=11)

                                          ttcttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                         >  6:384877/1‑149 (MQ=11)

                                            cttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                         <  4:223640/147‑1 (MQ=11)

                                            cttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                         >  7:303312/1‑147 (MQ=11)

                                            cttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                         <  5:363680/147‑1 (MQ=11)

                                             ttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCgg                                                                                                                                       <  4:79905/116‑1 (MQ=14)

                                             ttGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                      >  1:141186/1‑133 (MQ=11)

                                               gCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                      <  2:172036/147‑1 (MQ=11)

                                               gCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                    >  7:169404/1‑149 (MQ=11)

                                               gCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                    >  2:268231/1‑149 (MQ=11)

                                                ccGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                                                   <  7:222055/149‑1 (MQ=11)

                                                 cGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                  >  3:198689/1‑149 (MQ=11)

                                                  ggTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTcggg                                                                                                                             <  3:379108/121‑1 (MQ=11)

                                                  ggTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                 >  7:142004/1‑149 (MQ=11)

                                                   gTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGTGTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                  >  1:273849/1‑148 (MQ=11)

                                                   gTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                >  8:104964/1‑149 (MQ=11)

                                                   gTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                >  5:255329/1‑149 (MQ=11)

                                                   gTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                <  6:258761/149‑1 (MQ=11)

                                                   gTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                >  3:355451/1‑149 (MQ=11)

                                                             aCCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGtt                                                                                       <  6:58048/149‑1 (MQ=255)

GCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTC‑GCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCA‑GGCGT‑TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCA  >  NC_002947/5990305‑5990598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`