BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,990,469	G→A	N66N (AAC→AAT)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,990,469	0	G	A	96.4%	2175.9 / 62.5	1189	N66N (AAC→AAT)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (20/23); new base A (572/574); total (592/597)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 1190 total aligned reads displayed.

ACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGG‑GCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTCAACCAGCA  >  NC_002947/5990323‑5990617

acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAtt                                                                                                                                                    >  3:252656/1‑149 (MQ=17)

acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAtt                                                                                                                                                    <  5:344584/149‑1 (MQ=17)

 caacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAttc                                                                                                                                                   <  7:253692/149‑2 (MQ=17)

 caacaCATGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCATGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAttc                                                                                                                                                   <  8:278331/149‑2 (MQ=17)

  aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAttct                                                                                                                                                  >  8:186796/1‑147 (MQ=17)

  aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAttcg                                                                                                                                                  >  8:321823/1‑147 (MQ=17)

  aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAttcg                                                                                                                                                  >  1:225301/1‑147 (MQ=17)

  aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCAttcg                                                                                                                                                  >  2:136287/1‑147 (MQ=17)

   acacCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcgg                                                                                                                                                 <  2:277396/149‑1 (MQ=17)

   acacCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcgg                                                                                                                                                 <  5:262542/149‑1 (MQ=17)

   acacCTGCAATGATCAGCTGCTTCTTGCCGGGCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcgg                                                                                                                                                 <  4:214750/149‑1 (MQ=17)

    cacCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcggg                                                                                                                                                <  1:165123/149‑1 (MQ=17)

     acCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGc                                                                                                                                               <  3:344236/149‑1 (MQ=17)

     acCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGc                                                                                                                                               >  5:169584/1‑149 (MQ=17)

     acCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGc                                                                                                                                               >  2:388730/1‑149 (MQ=17)

     acCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAACGTACGGCGCA‑TCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGG‑GCCGTTGGGGc                                                                                                                                               >  5:70112/1‑149 (MQ=255)

      ccTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGcc                                                                                                                                              <  7:211929/149‑1 (MQ=17)

      ccTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGcc                                                                                                                                              <  2:330461/149‑1 (MQ=17)

       cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAATATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCt                                                                                                                                             >  3:104427/1‑149 (MQ=17)

       cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCt                                                                                                                                             >  7:176920/1‑149 (MQ=17)

       cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCAACGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCt                                                                                                                                             >  1:64830/1‑149 (MQ=14)

         gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTg                                                                                                                                           >  3:214971/1‑149 (MQ=14)

         gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTg                                                                                                                                           >  5:102213/1‑149 (MQ=14)

          cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCtt                                                                                                                                            <  7:155247/147‑1 (MQ=14)

          cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          >  6:40616/1‑149 (MQ=14)

          cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          >  7:130457/1‑149 (MQ=14)

          cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          <  7:336405/149‑1 (MQ=14)

          cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          >  7:367302/1‑149 (MQ=14)

          cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          <  1:139057/149‑1 (MQ=14)

           aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCt                                                                                                                                         >  3:141808/1‑149 (MQ=14)

           aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCt                                                                                                                                         <  1:315659/149‑1 (MQ=14)

           aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCt                                                                                                                                         >  1:361831/1‑149 (MQ=14)

           aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGGCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCt                                                                                                                                         <  3:179207/149‑1 (MQ=14)

            aTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGTT‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTc                                                                                                                                        <  1:102248/149‑1 (MQ=14)

             tGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTc                                                                                                                                        >  1:20211/1‑148 (MQ=14)

             tGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTCGCCAGGGCGGGCAATGTTCGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCg                                                                                                                                       <  2:105509/149‑1 (MQ=14)

             tGATCAGCTGCTTCTTGACGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTc                                                                                                                                        >  4:296122/1‑148 (MQ=14)

              gATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCg                                                                                                                                       >  4:104279/1‑148 (MQ=14)

               aTCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCg                                                                                                                                       >  8:255553/1‑147 (MQ=14)

               aTCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGaa                                                                                                                                     >  5:4838/1‑149 (MQ=14)

                tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGaa                                                                                                                                     <  4:166161/148‑1 (MQ=14)

                tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                                                                                    >  2:226927/1‑149 (MQ=14)

                tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                                                                                    >  4:48699/1‑149 (MQ=14)

                tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                                                                                    <  6:365637/149‑1 (MQ=14)

                tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                                                                                    >  8:52346/1‑149 (MQ=14)

                tCAGCTGCTTCTTGACGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                                                                                    <  4:142866/149‑1 (MQ=14)

                 cAGCTGCTTCTTGCCGTTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                                                                                    <  2:352374/148‑1 (MQ=14)

                  aGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCt                                                                                                                                  >  6:335415/1‑149 (MQ=14)

                  aGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCt                                                                                                                                  >  6:188234/1‑149 (MQ=14)

                  aGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCt                                                                                                                                  >  7:232609/1‑149 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTCCGGCGCA‑TCCGGGAACATCTCTTACAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                                                                                 >  6:296584/1‑149 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCt                                                                                                                                         <  4:234177/141‑1 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                                                                                 >  1:85250/1‑149 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                                                                                 <  3:53769/149‑1 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                                                                                 <  6:205991/149‑1 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTAGGGCACAAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                                                                                 >  5:318663/1‑149 (MQ=14)

                   gctgctTCTTGCCGGTCGCCTTCACAGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATCTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                                                                                 >  3:322725/1‑149 (MQ=14)

                    ctgctTCTTGCCGGTCGCCTTCGCCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                                >  7:58124/1‑149 (MQ=14)

                    ctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGaa                                                                                                                                     <  8:12956/144‑1 (MQ=14)

                    ctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                                >  4:336994/1‑149 (MQ=14)

                    ctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                                >  7:101473/1‑149 (MQ=14)

                    ctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                                >  6:235618/1‑149 (MQ=14)

                     tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTAAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                                               >  3:284159/1‑149 (MQ=14)

                      gctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                              >  5:370926/1‑149 (MQ=14)

                      gctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                              <  8:148878/149‑1 (MQ=14)

                       cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGcc                                                                                                                                              <  1:284099/133‑1 (MQ=14)

                       cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                                             >  5:211803/1‑149 (MQ=14)

                       cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                                             >  8:3635/1‑149 (MQ=14)

                       cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                              <  5:192288/148‑1 (MQ=14)

                       cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                              <  6:64025/148‑1 (MQ=14)

                        ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                                            >  5:392928/1‑149 (MQ=11)

                        cttcatccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                                            <  5:295245/143‑1 (MQ=11)

                         tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          >  1:262260/1‑134 (MQ=11)

                         tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGc                                                                                                                                          <  2:262260/134‑1 (MQ=11)

                          cttGCCGGTCGCCTTCATCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGcc                                                                                                                                              >  4:847/1‑129 (MQ=11)

                          cttGCCGGTCGCCTTCATCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGcc                                                                                                                                              <  3:847/129‑1 (MQ=11)

                          cttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                                            <  5:143630/147‑1 (MQ=11)

                          cttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                                            >  2:195202/1‑147 (MQ=11)

                           ttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcgg                                                                                                                                                 <  4:269611/125‑1 (MQ=11)

                           ttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                           >  5:235533/1‑147 (MQ=11)

                           ttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                                         >  8:213784/1‑149 (MQ=11)

                             gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGAGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                          <  1:40963/147‑1 (MQ=11)

                             gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                          <  6:135890/146‑1 (MQ=11)

                             gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAgg                                                                                                                        <  7:90328/148‑1 (MQ=11)

                             gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                                         <  2:172036/147‑1 (MQ=11)

                             gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                       >  7:289259/1‑149 (MQ=11)

                             gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGACGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                          <  5:91884/146‑1 (MQ=11)

                              ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                       <  2:312281/148‑1 (MQ=11)

                              ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGGGAGGAt                                                                                                                      >  4:124627/1‑149 (MQ=11)

                               cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTCTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCAGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                                     >  8:101000/1‑149 (MQ=11)

                                ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcggg                                                                                                                                                >  4:379108/1‑121 (MQ=11)

                                ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTcggg                                                                                                                                                <  3:379108/121‑1 (MQ=11)

                                ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                                    >  2:79952/1‑149 (MQ=11)

                                 gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                                   >  1:333390/1‑149 (MQ=11)

                                 gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                                   <  6:165552/149‑1 (MQ=11)

                                 gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                                   <  1:181005/149‑1 (MQ=11)

                                 gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                                   <  2:59882/149‑1 (MQ=11)

                                                        aTCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGG‑GCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGc                                                                                              >  6:260309/1‑147 (MQ=255)

                                                                                     cAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGG‑GCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTg                                                               <  7:31128/149‑1 (MQ=255)

                                                                                                                                      gCACCAGCGG‑GCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCtgg               >  8:75385/1‑149 (MQ=255)

ACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCA‑TCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGG‑GCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTCAACCAGCA  >  NC_002947/5990323‑5990617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`