| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 88.6% | 99.3 / ‑0.7 | 46 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (17/22); minor base A (5/0); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 95.7% | 120.7 / ‑6.1 | 46 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (22/22); minor base C (1/1); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCCA‑C‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499361‑1499644 || gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTAGCGGG‑CAcg‑cc > 8:4744/1‑149 (MQ=37) tGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCTCGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCcaca < 1:299499/149‑1 (MQ=21) cGGCCATTCAGAGCCTCAACGAAGCCTTGGCACCACAGCAGGACTGTGGCTGCCTAACGGTGGGAGGGGGCGTGCCCGCGAAACAGGCGG‑CGCGGTGAATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGa < 1:274379/149‑1 (MQ=21) gCCATTCAGCGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGAtt < 3:31486/149‑1 (MQ=21) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑ACTCCCACAGACTGATTGCCAtt < 3:330634/148‑1 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGAAACAGCCGC‑CCCGGTGCATGGCAC‑C‑GGCTTTGACGGTGTTCGCGGG‑CACGCCC‑GCTCCCACACACAGATTGCCAATAACCGGGc > 5:336191/1‑149 (MQ=18) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACAGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCGTTGCCCGTGTTAGCGGG‑CACGCCC‑GCTCCCACCGACTGATTGCCCTTTAGCGGGc > 1:273026/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 1:310546/149‑1 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTAGCGGG‑GACGCCC‑GCTCCCCCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAg > 8:285146/1‑149 (MQ=25) accacAGCAGGACTTAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 3:40447/149‑1 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGC‑CCCGGTGCCTTGCAC‑C‑CGCTTTTGCGGTGTTCGCGGGCCCCC‑CC‑GCTCCCCCAGACTGATTGGCCTTAATCGGGCCAATCGGTACAGTgg > 8:232790/1‑148 (MQ=16) gccTGAGGCTCCCAAAGGTTGGGAGCGGGCGTGCCCGCTAAACAGGCGC‑CGCGGTGCATGGAAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGa < 1:120108/106‑1 (MQ=14) gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGGATGGCAC‑C‑CGCATTGCCGGTGTTGGCGGG‑CCCGCCC‑CCGCCCACAGACTGATTGCCAATAAGCGGGGCTATAGCGACAGTTACGGCCCGgtt > 4:84180/1‑149 (MQ=18) cTGCGGCTGCAAAAGGGTGGGAGGGGGCGTGCCCGCTAAACCGGCGC‑CGCGG‑GGATGGTCACC‑GGTTTTGCGGGTGTTCGCGGG‑AACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt < 8:241208/149‑1 (MQ=255) cAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCCC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CCCGCCC‑GCGCCCCCAGACTGCTTGCCATTAAGCGGGCCTATGGCGACAGTGACGGCCCGGTTTTTGTTGCcgcg > 3:61986/1‑149 (MQ=18) cAAACGGTGGGAGCGGGCGTGACCGCGAAACAGGCGC‑CGCCGTGCATGGCAC‑C‑CGCGTTGCCCGTGTTACCGCG‑CACGCCC‑GCTCCCACAGACTGAATGCCATTAAGCGGGGCTAAGGCTACAGTGAAGGCCCGTTTTTTGTTGCCACg > 8:106976/1‑149 (MQ=255) aCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 3:284964/124‑1 (MQ=18) aCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCCGTGCATGGCAC‑C‑CGCATTGCCCGTGTTAGCCGG‑CACGCCC‑CCGCCCCCAGACAGATTGCCATTAAGCGGGCCGATAGCGACAGTgg > 4:284964/1‑123 (MQ=12) aGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 5:125995/1‑149 (MQ=25) aGCGGGCGTCCCGGCTAACCCGGCGC‑CGCGGTGCA‑GGCCA‑CGGGCTTTGCCGGTGTT‑CGGGGCCACG‑CCGGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 6:220837/149‑1 (MQ=255) gCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCGGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 3:84180/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:4744/149‑1 (MQ=25) tGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGCCGGCCCGTTTTTTGTTGCCTCGCTAACGACTCATGACTccc > 4:209523/1‑149 (MQ=25) gCCCGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 1:260380/1‑149 (MQ=37) cccGCGAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:104788/1‑148 (MQ=37) gcgAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 8:297421/1‑148 (MQ=37) gAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc > 5:223380/1‑142 (MQ=37) gAAACAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 6:223380/142‑1 (MQ=37) aaaCAGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 6:125995/149‑1 (MQ=37) aGGTGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 6:307118/1‑112 (MQ=21) aGGCGC‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 5:307118/112‑1 (MQ=21) gcgc‑CGCGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 7:287077/1‑148 (MQ=37) gc‑cgcGGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:73905/1‑149 (MQ=37) cgGTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 7:186512/1‑145 (MQ=37) ggTGCATGGCAC‑C‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt < 7:104788/149‑1 (MQ=38) acc‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc < 7:232790/147‑1 (MQ=38) acc‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 1:154898/3‑149 (MQ=38) c‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 7:73905/149‑1 (MQ=39) ctatcccGGTGTTCGCGCG‑CACGCCC‑GCTCCCACAGACTGAATGCCATTAAGCGGGCCAATCGCTACAGTGACGGCCCGGATTATGATGCCTCGCTACCGCAACAAGACACCCAGAGAACGCTATCACGCAGATCTTCACCGTcccca > 8:168460/6‑146 (MQ=255) cTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc < 7:297421/149‑1 (MQ=39) cTTTGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc < 8:314896/149‑1 (MQ=39) tttGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa > 4:136164/1‑67 (MQ=18) tttGCCGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 3:136164/67‑1 (MQ=18) ccGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt > 5:132424/1‑149 (MQ=255) ccGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt < 1:359268/149‑1 (MQ=255) ccGGTGTTCGCGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct < 7:285146/148‑1 (MQ=255) cgcGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa > 6:336690/3‑144 (MQ=255) cgcGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa < 5:336690/142‑1 (MQ=255) cgcGGG‑CACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAACTGAAACGACCCGACTTCTTCCATGAct > 2:149924/3‑148 (MQ=255) || GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCCA‑C‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499361‑1499644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |