Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,811	(T)7→6	intergenic (+48/+705)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,805	0	T	.	100.0%	147.2 / NA	33	intergenic (+42/+711)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/17);  total (16/17)

CACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT  >  NC_002947/4740659‑4740946                                                                                                                                                    |                                                                                                                                                 cACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTCGC‑CGAACGCAAGAAGGCTGCCCGAGGGCAGCCttc                                                                                                                                                <  6:254523/149‑2 (MQ=255) cACTGGAAAGAAATGCACTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCGAGGGCAGCCttc                                                                                                                                                <  4:29722/149‑2 (MQ=255)   gcggAAAGAAATGCTCTCCAGCCAGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttt                                                                                                                                              <  8:244144/147‑1 (MQ=255)       aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                          <  1:309410/149‑4 (MQ=255)       aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                          <  6:239264/149‑4 (MQ=255)                   tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑g                                                                                                                          >  8:67742/1‑149 (MQ=255)                   tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑g                                                                                                                          >  2:75733/1‑149 (MQ=255)                         ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCctct                                                                                                                    >  7:49749/1‑149 (MQ=255)                               aaGATCGGCCGTCCGCGCCATATGAACACAGGCGAGCAGAATCGCGACATCGTTGCACTGAAAGACCCCAAGTAAGCTCCAGTAGC‑CGAACGCAAGAAGGCTGCCCACCGGCAGCC‑TGTTTAATTTGTCCTGTGCC‑‑‑GGCCACATCGCgg                                                                                                              >  6:60977/1‑149 (MQ=255)                               aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCgg                                                                                                              <  2:263210/149‑1 (MQ=255)                               aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCgg                                                                                                              >  6:147616/1‑149 (MQ=255)                                aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGc                                                                                                             <  6:114227/149‑1 (MQ=255)                                 gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAGGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGcc                                                                                                            <  3:247950/149‑1 (MQ=255)                                  aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGcca                                                                                                           >  5:166095/1‑148 (MQ=255)                                      gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGcc                                                                                                       >  6:307742/1‑149 (MQ=255)                                            cgcgCCTGCTGTACACCGGCGAGCAGATGAGCGACAGCGTTGCCCTGATAGACCGCACGTAAGCTGCAGTAGC‑CTAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑TGCCTCTTCGCGGGCAAGGCCGCGaa                                                                                                 <  5:60977/149‑1 (MQ=255)                                            cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                 <  1:75733/149‑1 (MQ=255)                                             gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                <  7:272069/149‑1 (MQ=255)                                              cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                               <  1:157948/149‑1 (MQ=255)                                              cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                               <  5:323226/149‑1 (MQ=255)                                                 cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGGCAAGGCCGCGAAGAGGc                                                                                            <  5:119678/149‑1 (MQ=38)                                                 cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc                                                                                            <  8:49749/149‑1 (MQ=255)                                                    cTGTACACCGGCGAGCAGAAGGGCGACATCTTTGCCCTGAATGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGAC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcag                                                                                          <  4:239367/148‑3 (MQ=255)                                                      gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGGGAAGAGGCCGGca                                                                                       >  7:4239/1‑149 (MQ=255)                                                          acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcacacc                                                                                   >  3:115871/1‑147 (MQ=255)                                                          acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                   <  5:147616/149‑1 (MQ=255)                                                                    aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCGCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaa                                                                           >  1:74417/1‑148 (MQ=255)                                                                        gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCtttt                                                                                                                                               >  4:11155/1‑79 (MQ=255)                                                                        gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCtttt                                                                                                                                               <  3:11155/79‑1 (MQ=255)                                                                                       ccttcaaGCCCCCAAGTACGCTGCCGTAGC‑CCACCGCAAGACGCCGGCCCTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCC‑‑‑GGCCTCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                      <  7:124794/144‑1 (MQ=255)                                                                                                      gTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCGGGCCTCTTCGCGGCCTTGCCGGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCAGCCCGCTCACGCAGcc                                          >  1:95189/1‑149 (MQ=255)                                                                                                      gTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                            >  5:221055/1‑128 (MQ=255)                                                                                                         aGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                    <  1:84118/149‑1 (MQ=255)                                                                                                           cTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                  <  2:74417/149‑1 (MQ=255)                                                                                                            tGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                 >  1:58483/1‑149 (MQ=255)                                                                                                            tGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTAGCGGCCATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTATACGCCCCCACACGCAGCCCATTCAggg                                 >  2:178019/1‑149 (MQ=255)                                                                                                                                        ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                            >  6:144660/1‑95 (MQ=38)                                                                                                                                        ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                            <  5:144660/95‑1 (MQ=37)                                                                                                                                         cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt     >  2:18121/1‑149 (MQ=255)                                                                                                                                            gggCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt  <  2:58483/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT  >  NC_002947/4740659‑4740946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑