| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 88.2% | 94.9 / 8.0 | 34 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base C (12/18); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126500‑1126787 | gatgggCCTCTGCCGAGCTTGAACAGGTCTAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑c < 4:206109/144‑1 (MQ=39)gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGATGGTGGCTATACCTCCGCATTCGC‑GGG‑T‑AAA‑cc < 8:62479/148‑1 (MQ=25) gccgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTCCCGCGAATGCGGGGGTATAGCCCCCATCGCATTAGC‑GGG‑T‑AAA‑cccgcgcc > 6:286519/1‑143 (MQ=255) gccgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGCTTTAAACGCCAATGCGGAGGGTTAGCCACCCTAGCATTAGC‑AGG‑T‑AAA‑cacggaa > 5:88212/1‑142 (MQ=255) tgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGCCCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTacac < 1:35206/149‑1 (MQ=255) cGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGc < 3:296955/149‑1 (MQ=255) tCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCCAATGCGGAGGTATAGCCACCATCGCATTAGC‑GGGTT‑AAA‑CCGCTC‑CTACACAGAGCCCCCCCCCCCAGCCAGGTGGCg > 6:243160/1‑149 (MQ=255) gTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTCCCGCGCATGGGGAGGGTTTGGGACCATCGCCTTAGCGGGG‑T‑AAC‑CCGCTC‑CGACCCAGAGCCCGCCCCCCAAGCCAgggggggggg > 7:282712/1‑149 (MQ=255) aCAGGTGCACCGCCGCCCCGGTGGTTGGGCGCACAGTCCGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATCGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa < 4:174221/147‑1 (MQ=21) cATGGGGCCCTCCTGCCAGGGTGTTGGGTGCACATTGAGCCCTGTAGGCGCGGGATTACCCGCGAATGCGGAGGTATACCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 4:28986/149‑1 (MQ=37) tgGACCACCTGCCAGGTTGGTGGGTGGACATTGCGCCCTGTAGGCGCGGGGTTACCCGCGAATGCGGAGGTATAGCCACCAGCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt < 3:257445/149‑1 (MQ=38) tgttgGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGACGCCTGATAGcc < 5:129023/149‑2 (MQ=38) gttgGGTGGACATTGTGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGACGCCTGATAGcc < 1:180644/148‑2 (MQ=37) gggTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCCAAATCGGAGGTATAGCCAACATAGCATTATC‑GGG‑TAAAA‑CCGCAA‑ATACACAGAACCCGCCCCCCAAACCAAGAGGCGGGGAGGGCAATAACGCGTGCTGATaacagac > 4:228191/1‑142 (MQ=255) tGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGGGGAGGGTTAGGCACCCTCGCATTAGC‑GGGTT‑AAC‑CCGCTC‑CTACACAGAGCCCGCCACCCCAGCCAGGTGGCGGGGGGGGCTTTCAGGCGTGCTGATTGctg > 5:82221/1‑142 (MQ=255) gACATTGAGCCGTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGTCTATCAGGCGTCCTGATAGCTCtcgatcg < 6:88212/148‑1 (MQ=255) ggCGCGGTTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGT‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCacac < 7:108434/148‑1 (MQ=255) ggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 6:274673/136‑1 (MQ=255) ggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc > 5:274673/1‑136 (MQ=255) gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCtctc < 7:76027/120‑1 (MQ=255) gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCtctc > 8:76027/1‑120 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg > 5:101617/1‑149 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt < 4:284773/146‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt > 3:284773/1‑146 (MQ=255) cGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc > 6:291567/1‑138 (MQ=255) cGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc < 5:291567/138‑1 (MQ=255) gTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCCCACCAGGTTGCGGTCGCCGAACACGTTGTCg > 2:218937/1‑149 (MQ=255) gTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc < 3:228191/148‑1 (MQ=255) tataGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCGCCACACTGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGCATATCAGGCGACGTGATAGATGTCGAACGACGGGCAGGCGCACCCCAGGTTGCGGTCGCCGAACACGTTGCCg > 1:206829/1‑149 (MQ=255) aGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGttc > 6:320642/1‑123 (MQ=255) gCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 6:82182/1‑149 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CCACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGCCGACCCgg < 2:206829/149‑1 (MQ=255) caTCGCATTCGC‑GGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa < 6:101617/149‑1 (MQ=255) gc‑gGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCAcct < 6:256008/36‑2 (MQ=14) gc‑gGG‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCAcct > 5:256008/1‑35 (MQ=14) ggg‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg > 4:47942/1‑148 (MQ=255) ggg‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg > 7:163226/1‑148 (MQ=255) gg‑T‑AAACCCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa < 7:259548/149‑1 (MQ=255) | GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTC‑CTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126500‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |