| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,340,089 | 0 | G | C | 28.1% | 43.2 / 11.5 | 32 | intergenic (+74/‑257) | ribAB‑II/PP_3814 | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (15/8); new base C (5/4); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCT > NC_002947/4339951‑4340236 | ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc < 5:100915/149‑1 (MQ=25) gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg > 2:19501/1‑149 (MQ=25) tGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCATGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCggg > 6:185895/1‑149 (MQ=255) gCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGAGGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCATGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCggg < 5:185895/147‑1 (MQ=255) cGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTTACCCCCAATTCGGGGGTGGCACCACCGACGCCTTAGCgggga > 8:152717/1‑146 (MQ=255) gtggtgCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTTAACCCGAATGGGGGGGTGTGACACCCCACGCATTGGCGGGGTAA‑CCCCCGCCTaaacggg > 3:24506/1‑143 (MQ=255) cccGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCGCGAAAGCGAGGGTGACACCACCGCCCCATTCGCCGGTAAA‑CCCCCGCCTAAACGGGCCTTGGTGCgttt > 3:237643/1‑148 (MQ=255) ccGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg < 4:103675/113‑1 (MQ=18) ccGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg > 3:103675/1‑113 (MQ=18) cGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTTACCGCGCATTGGGGGGGGGCACCCCCCACCCATTAGCGGGGTAA‑ACCGCGCCGACACGGCCCGTGGTGCgttttt > 5:281416/1‑146 (MQ=255) gTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTTCCCGCGAATGCGACGGTGCCACCACCGACGCCTTAGCGGGTAAA‑CCCGCTCCTACAGGGCCCGTGGTGCGTTTGa > 7:80310/1‑147 (MQ=37) ccGGGGCTGGATGGCTGACAACACCCACGCAATTGATTTCGCGGGGCCCGGTAGGAGCGGGTTAACCCGCTACTGCGAGGGGGACACCACCCCCGCATGCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGa < 5:254721/149‑1 (MQ=25) ggggATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTTCCCGCGCATGCGAGGGTGACACCACCGACGCATTAGCGGGTAAA‑CCCGCGCCTAAAGGGCCCATGGTGGTTTTGACAGGGGGGAGt > 6:70740/1‑149 (MQ=255) acaacaaacaACAATTTGATTTCGGGGGCCCGTGTCGGCGGGGGTTACCCCGCTATGGCGAGGGGCACCCCCCCCCCCAATGCG‑GGGTAAACCCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGc < 7:152717/145‑1 (MQ=255) acacaAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCGCGAATGCGAGGGGGGCCCCACCCACGCCTTAGCGGGTAAA‑ACCGCGCCTACAGGGCCCATGGTGCGGTTTACAGGGGGGAGTGATTGGCCGG‑CGCCCCAAAc > 2:220658/1‑149 (MQ=18) cGGGGCCCTGTAGGAGCGGGTTTAACCCCGAATGCGAGGGTGACACCACCGACGCATTAGCGGGTAAA‑CCCGCTACTACCGGGCCCGTGGTGGGTTTGACAGGGCCGAGTGA‑AGGCCCGTCGCCCCATACCTAGTTCCAAAATCTTgg > 2:52970/1‑148 (MQ=25) ccTGTAGGAGCGGGTTTAACCCCGAATTCGAGGGTGACACCCCCGACGCATTCGCCGGTAAA‑CCCCCTCCAACAGGGCCCTTGGTGCTTTTTACAAGGCGGAGTGA‑TGGCCGGTAGCCCCAAAACACATGCCAAAAAATTTGAATaac > 6:148871/1‑146 (MQ=255) ggcgggggTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCataata < 5:148871/143‑1 (MQ=255) aGCGGTTTACCCCGCTAATGCGAGGGTGACACCACCGACGAATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGa < 1:52970/149‑1 (MQ=39) aCCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCaaaa > 8:256141/1‑119 (MQ=255) ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGa > 5:113660/1‑149 (MQ=255) ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGa > 4:195553/1‑149 (MQ=255) ttGCTAGGGTGACACCACCGACGCATTGGGGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGGCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGa < 1:220658/148‑1 (MQ=255) ttGCGAGGGGTCCACCACCGACCCATTCGCGGTTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGa < 4:24506/148‑1 (MQ=255) tGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTCCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCCTACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCACGTAGACCTTGAg > 7:120407/1‑149 (MQ=255) gAGGGTGACACCACCGCCGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCGTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGaa > 4:39395/1‑115 (MQ=255) gAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCt > 2:234232/1‑149 (MQ=255) ggTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGc < 6:281416/148‑1 (MQ=255) acacCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGcc < 5:70740/149‑1 (MQ=255) caccGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGTTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACAATAATTTGAAATCCCGTAGACATTGAGTCTGCAGGCCGGCa > 8:105641/1‑149 (MQ=255) accGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAc > 6:207240/1‑149 (MQ=255) cGACGCATTCGGGGGTAAA‑CCCGCTCCTACAGGGCCCGTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCt < 4:237643/149‑1 (MQ=255) | GGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAA‑CCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGA‑TGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCT > NC_002947/4339951‑4340236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |