| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,740,816 | T→G | intergenic (+53/+700) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,816 | 0 | T | G | 97.1% | 113.8 / ‑6.5 | 35 | intergenic (+53/+700) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (16/18); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740677‑4740954 | tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑g > 8:67742/1‑149 (MQ=255)tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑g > 2:75733/1‑149 (MQ=255) ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCctct > 7:49749/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCATATGAACACAGGCGAGCAGAATCGCGACATCGTTGCACTGAAAGACCCCAAGTAAGCTCCAGTAGC‑CGAACGCAAGAAGGCTGCCCACCGGCAGCC‑TGTTTAATTTGTCCTGTGCC‑‑‑GGCCACATCGCgg > 6:60977/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCgg > 6:147616/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCgg < 2:263210/149‑1 (MQ=255) aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGc < 6:114227/149‑1 (MQ=255) gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAGGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGcc < 3:247950/149‑1 (MQ=255) aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGcca > 5:166095/1‑148 (MQ=255) gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGcc > 6:307742/1‑149 (MQ=255) cgcgCCTGCTGTACACCGGCGAGCAGATGAGCGACAGCGTTGCCCTGATAGACCGCACGTAAGCTGCAGTAGC‑CTAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑TGCCTCTTCGCGGGCAAGGCCGCGaa < 5:60977/149‑1 (MQ=255) cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGaa < 1:75733/149‑1 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGGCAAGGCCGCGAag < 7:272069/149‑1 (MQ=255) cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAaga < 1:157948/149‑1 (MQ=255) cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAaga < 5:323226/149‑1 (MQ=255) cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGGCAAGGCCGCGAAGAGGc < 5:119678/149‑1 (MQ=38) cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc < 8:49749/149‑1 (MQ=255) cTGTACACCGGCGAGCAGAAGGGCGACATCTTTGCCCTGAATGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGAC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcag < 4:239367/148‑3 (MQ=255) gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGGGAAGAGGCCGGca > 7:4239/1‑149 (MQ=255) acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcacacc > 3:115871/1‑147 (MQ=255) acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa < 5:147616/149‑1 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCGCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaa > 1:74417/1‑148 (MQ=255) ccttcaaGCCCCCAAGTACGCTGCCGTAGC‑CCACCGCAAGACGCCGGCCCTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCC‑‑‑GGCCTCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc < 7:124794/144‑1 (MQ=255) gTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCGGGCCTCTTCGCGGCCTTGCCGGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCAGCCCGCTCACGCAGcc > 1:95189/1‑149 (MQ=255) gTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct > 5:221055/1‑128 (MQ=255) aGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa < 1:84118/149‑1 (MQ=255) cTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg < 2:74417/149‑1 (MQ=255) tGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg > 1:58483/1‑149 (MQ=255) tGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTAGCGGCCATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTATACGCCCCCACACGCAGCCCATTCAggg > 2:178019/1‑149 (MQ=255) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct < 5:144660/95‑1 (MQ=37) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct > 6:144660/1‑95 (MQ=38) cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt > 2:18121/1‑149 (MQ=255) gggCAGCC‑TTTTTTATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt < 2:58483/149‑1 (MQ=255) caagccttttttATTTGGCCTGTGCC‑‑‑GGCCGCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt < 6:166095/141‑1 (MQ=255) ttttttATTTGTCCTGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca > 8:208194/1‑149 (MQ=255) | TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGC‑CGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCC‑‑‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740677‑4740954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |