Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	123.9 / NA	37	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499058‑499346                                                                                                                                                  |                                                                                                                                                 aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCggg                                                                                                                                                >  3:70501/1‑148 (MQ=255)   gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                                             >  2:200340/1‑149 (MQ=255)   gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCCCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                                             >  7:292139/1‑149 (MQ=255)       aTCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                                         <  2:254831/149‑1 (MQ=255)               ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                                 >  7:185363/1‑149 (MQ=255)                   aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                             <  7:290638/149‑1 (MQ=255)                     ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctgg                                                                                                                           >  6:102018/1‑149 (MQ=255)                      gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAcgggccgggc                                                                                                                          >  8:65005/1‑149 (MQ=255)                        aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctggcct                                                                                                                        <  3:276606/149‑1 (MQ=255)                         cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg                                                                                                                       >  4:106101/1‑149 (MQ=255)                           ccTGATGCAGAAGTTGAAAGTGAACTCTCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGTGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                                     <  8:292139/149‑1 (MQ=255)                           ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                                     <  1:200340/149‑1 (MQ=255)                                    gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                                 >  6:192986/1‑128 (MQ=255)                                    gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                                 <  5:192986/128‑1 (MQ=255)                                           aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                               >  1:285058/1‑139 (MQ=255)                                                 aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                                <  7:123175/148‑1 (MQ=255)                                                 aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                                >  6:120008/1‑148 (MQ=255)                                                 aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                                <  3:292297/148‑1 (MQ=255)                                                          gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCgg                                                                                     <  5:11569/149‑1 (MQ=255)                                                           cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCgg                                                                                     <  8:185363/148‑1 (MQ=255)                                                              aGCTGGTGAAGGACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCAt                                                                                 <  5:183578/149‑1 (MQ=255)                                                                    tGAAGTACGCCATGGAGCACAGGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATATCTATGGCTGAGCCGGGCTC‑TTCGCGGGCATGCCCGc                                                                           >  5:114229/1‑149 (MQ=255)                                                                            gCCATGGAGCACAAGCTTATCTGATTGCGAATTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGAATGCCCGCTCCCACAg                                                                   <  6:240526/149‑1 (MQ=255)                                                                            gCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGGCTTGCCAGCTGAAATCTATTGCTGAGCCGGCCACATACCCCGGCAT‑CCCGCTGCcacac                                                                   >  7:190122/1‑148 (MQ=255)                                                                              cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGcc                                                                              >  7:162564/1‑136 (MQ=255)                                                                              cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGcc                                                                              <  8:162564/136‑1 (MQ=255)                                                                                tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGTGATCGGGCATGCCCGCTAACCGCCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATTACCCCTCACACACGttt                                                               >  5:142075/1‑149 (MQ=255)                                                                                  gAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAACCTTGCCCAAGCCTATGTGGGCGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTc                                                              <  5:71282/148‑1 (MQ=39)                                                                                      acaAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACACGTTTCGTGGt                                                         >  2:202310/1‑149 (MQ=255)                                                                                                           aTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAGGCCCGCTAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCaa                                    <  5:120008/149‑1 (MQ=255)                                                                                                               ggggGCCCTAAACTTGCCGAAGCCTATGTGGGAGGGGGCATGCCCGCGAACGGGCCTGGCCTGCTTATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATccc                                <  4:70501/149‑1 (MQ=39)                                                                                                                 gTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCTGGCCTGGGGTTGTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATTCCCCCTCCCACAGGTTTCGTGGGTGGCGAGCAAGGGCATTACAAACCCGc                              >  2:224774/1‑149 (MQ=255)                                                                                                                     ccGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGGTGATCTCTATTGCTGAGCCGGCCTC‑TTAGCGGGCATGCCCGCGCCCCCAGGTTTCGTGGTTGGCTCGCAAGGGCCTTACAATACCGCCAta                          >  2:190076/1‑148 (MQ=255)                                                                                                                          aCTTGCCGAAGCCTTTGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTGTGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGt                     <  3:106101/149‑1 (MQ=255)                                                                                                                            ttGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCTGGCCGGGTGATCTCTATGGCTGAGCCGGCCTC‑TTAGCGGGGATGCCCGCTCCCCCAGGTTTAGTGGTTGGGTCGCAAGGGCCTTCCAATACCGCCATACTTGTAg                   >  4:180096/1‑149 (MQ=255)                                                                                                                            ttGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCGGCTGATCTATATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTGCCACAGGTTTAGTGGTTGGCTCGCAAGGGGATTCCAATCCCGCCATCCTTGTAg                   >  2:83308/1‑149 (MQ=39)                                                                                                                              gCCCAAGCCTATGTGGGCGCGGGCATGCCCGCGAACGGGCCTGCCCTGCTGATCTCTATGGCTGCGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg                  <  3:180096/148‑1 (MQ=39)                                                                                                                                             ggAGCGGGCATGCCCGCGACCGGGCCGGGCCGGCTGAGCTCTAGGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTAcaacaa  <  7:65005/149‑1 (MQ=39)                                                                                                                                                  |                                                                                                                                                 AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC‑TTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499058‑499346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑