Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,030,808	Δ1 bp	8.7%	coding (866/1353 nt)	PP_0887 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,030,808	0	A	.	8.7%	207.3 / 14.5	69	coding (866/1353 nt)	PP_0887	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (31/32);  new base . (2/4);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

CCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTG  >  NC_002947/1030670‑1030956                                                                                                                                            |                                                                                                                                                     ccgcaCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTTGCAAAGTCTCTTTGC‑ACAGCtttac                                                                                                                                            <  5:63207/149‑5 (MQ=255)  cgccCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTTGCAAAGTCTCTTTGC‑ACAGCtttacc                                                                                                                                           <  5:358229/149‑6 (MQ=255)   gccCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTgcg                                                                                                                                          >  2:95232/1‑149 (MQ=255)    cccATGAACTGGGTACGCCACTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTgcgc                                                                                                                                         <  6:231561/149‑1 (MQ=255)     ccATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGTTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTgcgcg                                                                                                                                        >  8:217597/1‑149 (MQ=255)      cATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTgcgcg                                                                                                                                        >  7:22728/1‑148 (MQ=255)       aTGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCa                                                                                                                                      <  1:95232/149‑1 (MQ=255)          aaCTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGcc                                                                                                                                   >  6:231088/1‑149 (MQ=255)                gTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGc                                                                                                                              >  8:267295/1‑148 (MQ=255)                           gCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCt                                                                                                                  <  5:231088/149‑1 (MQ=255)                           gCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCt                                                                                                                  >  8:163940/1‑149 (MQ=255)                            cGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTg                                                                                                                 <  3:332940/149‑1 (MQ=255)                             gACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTgg                                                                                                                >  2:284885/1‑149 (MQ=255)                             gACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTgg                                                                                                                <  7:212873/149‑1 (MQ=255)                             gACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTgg                                                                                                                >  7:106160/1‑149 (MQ=255)                              aCCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGc                                                                                                               >  4:300498/1‑149 (MQ=255)                                cATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCACCAGCTGGTGCGCGCAGCCGAAGCCCACCGGCGCCTGGc                                                                                                               >  8:87182/1‑147 (MQ=255)                                  tGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATc                                                                                                           >  8:91723/1‑149 (MQ=255)                                     gCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTg                                                                                                        <  4:56692/149‑1 (MQ=255)                                        tGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAg                                                                                                     <  7:267295/149‑1 (MQ=255)                                              tCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGCAGCAGGAt                                                                                               <  8:22728/149‑1 (MQ=255)                                                       tGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGcc                                                                                      >  6:332296/1‑149 (MQ=255)                                                             aGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctggctg                                                                                <  7:217597/149‑1 (MQ=255)                                                             aGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctggctg                                                                                >  4:340348/1‑149 (MQ=255)                                                                aCCACACCGCCCCGCTGTTGCTGGAGGACGTGCAAATCCTCCAGGACCTGGTATCGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCATCCGCCGCCTTGCGATCGTGGAGGAGGATGGGACCGCCTGGCTGGAc                                                                             <  4:27313/149‑1 (MQ=255)                                                                 ccACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGAc                                                                             >  6:165269/1‑148 (MQ=255)                                                                  cacaCCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGAc                                                                             >  6:33500/1‑147 (MQ=255)                                                                    cacCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAgg                                                                         <  7:91723/149‑1 (MQ=255)                                                                       cGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGgcgc                                                                      <  1:284885/149‑1 (MQ=255)                                                                         aCCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGcc                                                                                      >  6:280413/1‑131 (MQ=255)                                                                         aCCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGcc                                                                                      <  5:280413/131‑1 (MQ=255)                                                                         aCCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCt                                                                     >  5:249194/1‑148 (MQ=255)                                                                             gCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAcc                                                                >  4:292192/1‑149 (MQ=255)                                                                                 ttGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGcc                                                                                                                                   >  1:169998/1‑78 (MQ=255)                                                                                 ttGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGcc                                                                                                                                   <  2:169998/78‑1 (MQ=255)                                                                                 ttGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCt                                                             <  4:265719/148‑1 (MQ=255)                                                                                   gCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGc                                                          >  7:176249/1‑149 (MQ=255)                                                                                    cAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCa                                                         >  3:120926/1‑149 (MQ=255)                                                                                         ggaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGa                                                    <  5:33500/149‑1 (MQ=255)                                                                                         ggaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGa                                                    >  5:165851/1‑149 (MQ=255)                                                                                          gaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctggctg                                                                                >  1:321840/1‑120 (MQ=255)                                                                                          gaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctggctg                                                                                <  2:321840/120‑1 (MQ=255)                                                                                            ccTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATgc                                                 >  3:192963/1‑149 (MQ=255)                                                                                               gCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGcc                                              >  7:66404/1‑149 (MQ=255)                                                                                                cAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGt                                                                                                         >  1:128790/1‑89 (MQ=255)                                                                                                cAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGt                                                                                                         <  2:128790/89‑1 (MQ=255)                                                                                                cAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATgcgc                                               <  5:165269/147‑1 (MQ=255)                                                                                                  aaTCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCgg                                           <  7:190797/149‑1 (MQ=255)                                                                                                    tcctccAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGaa                                         <  6:165851/149‑1 (MQ=255)                                                                                                         cAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCa                                     <  6:249194/148‑1 (MQ=255)                                                                                                            gACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTa                                 <  8:66404/149‑1 (MQ=255)                                                                                                            gACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTa                                 >  4:129939/1‑149 (MQ=255)                                                                                                               cAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCg                              <  8:106160/149‑1 (MQ=255)                                                                                                                aGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGa                                                                           <  4:123713/103‑1 (MQ=255)                                                                                                                aGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGa                                                                           >  3:123713/1‑103 (MQ=255)                                                                                                                aGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCt                                                             >  8:64974/1‑117 (MQ=255)                                                                                                                aGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCt                                                             <  7:64974/117‑1 (MQ=255)                                                                                                                   tAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTc                          <  7:163940/149‑1 (MQ=255)                                                                                                                     caGCTGTTGCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGAt                                                   <  4:104829/121‑1 (MQ=255)                                                                                                                     caGCTGTTGCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGAGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGAt                                                   >  3:104829/2‑122 (MQ=255)                                                                                                                     aaGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCa                        <  4:192963/149‑1 (MQ=255)                                                                                                                       gctgttgCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCt                            >  5:167885/6‑143 (MQ=255)                                                                                                                       gctgttgCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCt                            <  6:167885/138‑1 (MQ=255)                                                                                                                       gCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgc                      <  3:300498/149‑1 (MQ=255)                                                                                                                        cTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgcg                     >  5:39962/1‑149 (MQ=255)                                                                                                                            tgCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCt                  <  2:164280/149‑1 (MQ=255)                                                                                                                            tgCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCt                  <  6:39962/149‑1 (MQ=255)                                                                                                                                      cTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGc        <  8:176249/149‑1 (MQ=255)                                                                                                                                       tttGCAACAGCTGGTGCGCGCAGCCAAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGGGGCGCTCAACCGCTGGCACCTGATGCGCCCGGCAGCGAGCTCCCGCATCAAGCGCCTGCGCGCCgac        >  4:266516/1‑146 (MQ=255)                                                                                                                                            aaCAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTg  >  8:137135/1‑149 (MQ=255)                                                                                                                                            aaCAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTg  <  3:292192/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                     CCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTG  >  NC_002947/1030670‑1030956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑