Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	61,265	Δ1 bp	10.5%	coding (435/885 nt)	PP_0052 →	beta‑lactamase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	61,265	0	C	.	10.5%	107.9 / 8.1	38	coding (435/885 nt)	PP_0052	beta‑lactamase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (18/15);  new base . (2/2);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

GCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTACCGC  >  NC_002947/61120‑61412                                                                                                                                                   |                                                                                                                                                    gcgcgcACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGAAGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTCCGT‑TGCACCCg                                                                                                                                                  >  8:114603/1‑149 (MQ=255)       acaTTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTggg                                                                                                                                            <  5:48811/149‑1 (MQ=255)         aTTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGca                                                                                                                                          >  6:96709/1‑149 (MQ=255)          ggcatcggccccggggaagtcgcagcgggcggtgccatagtcgggcatgaacaaggtgtctccaacgaacaccgcgatctcgccggcatcttcgatcatgaaactcatgcacgcgggtgtgtGCCCGTGCGT‑TGCACACGCCTGGGcac                                                                                                                                         >  7:137451‑M1/123‑149 (MQ=255)                     cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGt                                                                                                                               <  5:88367/148‑1 (MQ=255)                     cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTg                                                                                                                              >  5:46313/1‑149 (MQ=255)                     cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTg                                                                                                                              >  5:51510/1‑149 (MQ=255)                         aaGTGGTTGGCGCCTTGTTCAACGCAGAGCACGGCTTCGCTCTTGATGGCAGCCAGTTCGATGTGCTTCTCGAAGACGAGGAAGGTTTCATGATTTGGAAACTTCAGTCCCGTGCTT‑TGCAGTCGCCTTGGCACACACGCGGGTGCAt                                                                                                                           <  4:191496/148‑1 (MQ=255)                                     ccTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATc                                                                                                              >  6:71645/1‑149 (MQ=255)                                         gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                                           <  6:46313/148‑1 (MQ=255)                                         gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                                           <  5:96709/148‑1 (MQ=255)                                         gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                                           <  6:51510/148‑1 (MQ=255)                                          ttCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATc                                                                                                              <  4:71174/144‑1 (MQ=255)                                          ttCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGagat                                                                                                         >  3:71174/1‑146 (MQ=255)                                            cAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGAt                                                                                                        <  3:171135/148‑1 (MQ=255)                                                          gCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                                         <  2:310839/149‑1 (MQ=255)                                                              cGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGgt                                                                                       <  4:275658/147‑1 (MQ=255)                                                                 ccGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCg                                                                                  >  5:226681/1‑149 (MQ=255)                                                                   gTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATc                                                                                            >  1:41507/1‑137 (MQ=255)                                                                   gTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATc                                                                                            <  2:41507/137‑1 (MQ=255)                                                                    tGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACTCACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTg                                                                               <  4:321431/149‑1 (MQ=255)                                                                      aTGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGga                                                                             <  2:131860/149‑1 (MQ=255)                                                                       tGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCC‑GGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATAGCGGTGTTCGTTGgaga                                                                           >  3:35350/1‑149 (MQ=255)                                                                              cAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCtt                                                                     >  8:14773/1‑149 (MQ=255)                                                                                   cGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTc                                                                 <  6:160457/148‑1 (MQ=255)                                                                                                gAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTAt                                                     <  5:71645/147‑1 (MQ=255)                                                                                                gAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATgg                                                   >  7:311041/1‑149 (MQ=255)                                                                                                         gAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCAccgcccg                                          >  4:198833/1‑149 (MQ=255)                                                                                                              tttCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCg                                     >  4:32935/1‑149 (MQ=255)                                                                                                                         gCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCggg                          >  3:96665/1‑149 (MQ=255)                                                                                                                         gCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCggg                          >  3:27011/1‑149 (MQ=255)                                                                                                                          gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                                         <  5:185936‑M1/75‑1 (MQ=255)                                                                                                                          gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                                         >  6:185936‑M1/11‑85 (MQ=255)                                                                                                                          gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgc                                                                                          >  8:210194‑M1/11‑84 (MQ=255)                                                                                                                          gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgc                                                                                          <  7:210194‑M1/74‑1 (MQ=255)                                                                                                                          cAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCgggg                         >  1:197529/1‑149 (MQ=255)                                                                                                                                           cGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATg                                                    >  8:137701/1‑105 (MQ=255)                                                                                                                                             t‑tGCACACGCC‑GGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATAGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTAc     <  4:35350/149‑1 (MQ=255)                                                                                                                                                  acacGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTACCGc  >  4:239371/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTACCGC  >  NC_002947/61120‑61412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑