Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,054,193:1	+T	10.0%	coding (767/1320 nt)	PP_3573 →	monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,054,193	1	.	T	10.0%	216.4 / 21.1	60	coding (767/1320 nt)	PP_3573	monooxygenase
Reads supporting (aligned to +/- strand):  ref base . (27/27);  new base T (2/4);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

ACCGCAACCGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTGGAC  >  NC_002947/4054046‑4054341                                                                                                                                                      |                                                                                                                                                     aCCGCAACCGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAggg                                                                                                                                                       >  1:140122/1‑148 (MQ=255)  ccGCAACCGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑Ca                                                                                                                                                    <  6:142581/149‑1 (MQ=255)   cGCAACCGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CAt                                                                                                                                                   >  8:233253/1‑149 (MQ=255)    gCAACCGCGACTTCTGCTGGTGGCTGGGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CAt                                                                                                                                                   >  1:185325/1‑149 (MQ=255)       aCCGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGAcc                                                                                                                                               <  1:154840/149‑1 (MQ=255)        ccGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGAccc                                                                                                                                              <  7:177927/149‑1 (MQ=255)          gcgACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTg                                                                                                                                            >  5:154740/1‑149 (MQ=255)                 ctgctgGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCGACCAGGGTCATG‑CCCTGGTGGGc                                                                                                                                      <  7:179636/148‑1 (MQ=255)                    ctgGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCGACCAGGGTCATG‑CCCTGGTGGGcgag                                                                                                                                   <  4:76382/148‑4 (MQ=255)                       gtggCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGAc                                                                                                                                 <  1:125029/147‑1 (MQ=255)                                gCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTTGAGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       <  7:233253/149‑1 (MQ=255)                                gCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       <  4:286095/149‑1 (MQ=255)                                gCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       >  1:19605/1‑149 (MQ=255)                                gCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CAGGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       <  8:170680/149‑1 (MQ=255)                                   tGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGaa                                                                                                                    <  5:274666/149‑1 (MQ=255)                                    gCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGaa                                                                                                                    >  3:190275/1‑148 (MQ=255)                                     cTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGGTCATG‑CCCTGGTggg                                                                                                                                       >  5:231202/1‑128 (MQ=255)                                     cTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGGTCATG‑CCCTGGTggg                                                                                                                                       <  6:231202/128‑1 (MQ=255)                                      tGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAAc                                                                                                                 >  2:163682/1‑149 (MQ=255)                                           gAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       >  8:178348/1‑138 (MQ=255)                                           gAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       >  2:300900/1‑138 (MQ=255)                                           gAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       <  7:178348/138‑1 (MQ=255)                                           gAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTc                                                                                                                       <  1:300900/138‑1 (MQ=255)                                                    gcgGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCtt                                                                                                   >  8:163714/1‑149 (MQ=255)                                                      ggAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTcc                                                                                                 <  3:129389/149‑1 (MQ=255)                                                      ggAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTc                                                                                                  >  4:129389/1‑148 (MQ=255)                                                      ggAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTc                                                                                                  >  7:338664/1‑148 (MQ=255)                                                        aaaTTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAg                                                                                               <  4:190275/149‑1 (MQ=255)                                                              ccAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAc                                                                                         >  3:92690/1‑149 (MQ=255)                                                              ccAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAc                                                                                         <  7:163714/149‑1 (MQ=255)                                                               cAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAcc                                                                                        >  3:161571/1‑149 (MQ=255)                                                               cAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAAcc                                                                                        >  5:272929/1‑149 (MQ=255)                                                                      ggTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTc                                                                                   >  6:242092/1‑147 (MQ=255)                                                                      ggTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCga                                                                                 <  4:92690/149‑1 (MQ=255)                                                                          gcgAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTcgc                                                                                                         <  2:198160/121‑1 (MQ=255)                                                                           cgAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACa                                                                            <  8:338664/149‑1 (MQ=255)                                                                                  gtgACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATcg                                                                     >  1:180461/1‑149 (MQ=255)                                                                                  gtgACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATcg                                                                     >  2:124147/1‑149 (MQ=255)                                                                                        aTCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcga                                                               <  6:154740/149‑1 (MQ=255)                                                                                         tCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcgac                                                              >  3:9483/1‑149 (MQ=255)                                                                                         tCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcgac                                                              <  1:163682/149‑1 (MQ=255)                                                                                           gcgGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGcgacg                                                             <  1:124147/148‑1 (MQ=255)                                                                                              gTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACaa                                                         <  4:161571/149‑1 (MQ=255)                                                                                                      cgcCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTgg                                                 <  4:9483/149‑1 (MQ=255)                                                                                                              gtggCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCctgct                                          <  5:242092/148‑1 (MQ=255)                                                                                                                 gCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGAt                                      >  2:25052/1‑149 (MQ=255)                                                                                                                  ccACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAAc                                                        >  7:56512/1‑130 (MQ=255)                                                                                                                  ccACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAAc                                                        <  8:56512/130‑1 (MQ=255)                                                                                                                          tGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCctgct                                          >  4:307739/1‑136 (MQ=255)                                                                                                                          tGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCctgct                                          <  3:307739/136‑1 (MQ=255)                                                                                                                            gACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATg                            <  2:185325/148‑1 (MQ=255)                                                                                                                               ttCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGc                           <  2:112886/146‑1 (MQ=255)                                                                                                                               ttCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGc                           >  1:112886/1‑146 (MQ=255)                                                                                                                                      cccTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGaa                 <  6:272929/149‑1 (MQ=255)                                                                                                                                       ccTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAAc                >  7:34718/1‑149 (MQ=255)                                                                                                                                        cTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACg               >  7:62981/1‑149 (MQ=255)                                                                                                                                        cTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACg               >  2:262672/1‑149 (MQ=255)                                                                                                                                         aggCCGACCAGGGTCATG‑CCCTGGTGGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGcc                          <  6:167698/135‑1 (MQ=255)                                                                                                                                         aggCCGACCAGGGTCATG‑CCCTGGTGGGCCAGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTAGAtaggaa                >  5:167698/3‑142 (MQ=255)                                                                                                                                           gCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCaa            >  5:70364/1‑149 (MQ=255)                                                                                                                                              caccaGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACgg         >  4:47353/1‑149 (MQ=255)                                                                                                                                                   ggg‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTgg    <  3:47353/149‑1 (MQ=255)                                                                                                                                                     g‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTGGAc  <  2:180461/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     ACCGCAACCGCGACTTCTGCTGGTGGCT‑GGGCGTGCTGGGCGAGTGGGACGCGGAAATTGCCAAGCCCGGTCGCGAGCATGTGACCATCGCGGTCAGTGGCGCCCGTGGTGGCCACACCGTGGACTTCCGTGCCCTCGCCCACCAGGG‑CATGACCCTGGTCGGCCTGACCCAGTCGTTCGAAAACGGCGTCGCGCGCTTCCAGGACAACCTGGTCGAGAACATCAATCGCGGCGACGACAACTACCTGGCCCTGCTGGATGCGGCCGATGCCTACATCGAACGCAACGGCCTGGAC  >  NC_002947/4054046‑4054341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑