Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947307,8651.C98.1% 173.9 / ‑3.2 52intergenic (+880/‑989)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (24/27);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307721‑308008
                                                                                                                                                 |                                                                                                                                                
ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                              <  8:23567/149‑4 (MQ=255)
 cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtt                                                                                                                                             <  1:1184/149‑5 (MQ=255)
   tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTAc                                                                                                                                           >  3:57774/1‑149 (MQ=255)
       ctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGa                                                                                                                                       >  5:107831/1‑149 (MQ=255)
          ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATc                                                                                                                                     <  3:129585/148‑1 (MQ=255)
          ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCg                                                                                                                                    <  6:350996/149‑1 (MQ=255)
              cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTga                                                                                                                                <  7:323729/149‑1 (MQ=255)
              cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTg                                                                                                                                 <  4:48621/148‑1 (MQ=255)
               aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGGGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGAGTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTgac                                                                                                                               <  4:337338/149‑1 (MQ=255)
                             ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                                 <  6:107831/149‑1 (MQ=255)
                             ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                                 >  5:83360/1‑149 (MQ=255)
                                      gATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCTATGTGACCTTGTTCTTCGGCATGTCCGGAAATGGCAAGACTACCCTTTCGGCTGACGAAAG‑CAGTTACATGATCGGTGAAGACGAGCACGGTTTGGGAGAAgg                                                                                                        >  6:264280/1‑149 (MQ=255)
                                            cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                                  >  8:289945/1‑149 (MQ=255)
                                              gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                                >  7:339137/1‑149 (MQ=255)
                                               ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTgacg                                                                                                                              <  4:64355/118‑1 (MQ=255)
                                               ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTgacg                                                                                                                              >  3:64355/1‑118 (MQ=255)
                                               ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                                 <  2:18552/147‑1 (MQ=255)
                                               ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                               <  5:347108/149‑1 (MQ=255)
                                               ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                                <  7:289945/148‑1 (MQ=255)
                                                cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                              <  7:21811/149‑1 (MQ=255)
                                                      aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATg                                                                                        >  6:62681/1‑149 (MQ=255)
                                                      aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATg                                                                                        >  4:43923/1‑149 (MQ=255)
                                                        tGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                         <  4:178961/146‑1 (MQ=255)
                                                        tGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                         >  3:178961/1‑146 (MQ=255)
                                                              gCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                                <  7:210597/149‑1 (MQ=255)
                                                               ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                               >  4:302666/1‑149 (MQ=255)
                                                                 aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                    >  1:76082/1‑126 (MQ=255)
                                                                 aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                    <  2:76082/126‑1 (MQ=255)
                                                                        gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       >  7:260105/1‑148 (MQ=255)
                                                                          gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGTGAAGGCGTTGTCTTCAACa                                                                                          >  5:314449/1‑127 (MQ=255)
                                                                          gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGTGAAGGCGTTGTCTTCAACa                                                                                          <  6:314449/127‑1 (MQ=255)
                                                                                    aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                          <  8:260105/149‑1 (MQ=255)
                                                                                         aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGcc                                                                      <  1:160447/132‑1 (MQ=255)
                                                                                         aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGcc                                                                      >  2:160447/1‑132 (MQ=255)
                                                                                          ccTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    <  4:57774/149‑1 (MQ=255)
                                                                                           cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    >  1:129480/1‑148 (MQ=255)
                                                                                             tGTTCTTCGGCCTGTCCGGCACTGGCAAAACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    >  3:181610/1‑146 (MQ=255)
                                                                                               ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGACGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaa                                               <  3:16288/149‑1 (MQ=255)
                                                                                                tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                  <  6:333642/129‑1 (MQ=255)
                                                                                                tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                  >  5:333642/1‑129 (MQ=255)
                                                                                                tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                                 >  3:96101/1‑146 (MQ=255)
                                                                                                      gCCTGTCCGGCACTGTCAAGACTACCCTTTCGTCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTTGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAg                                        <  5:264280/149‑1 (MQ=255)
                                                                                                        cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGcc                                      >  5:59421/1‑149 (MQ=255)
                                                                                                             cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATTGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTc                                  <  6:59421/148‑1 (MQ=255)
                                                                                                                cacaGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                              <  4:96101/149‑1 (MQ=255)
                                                                                                                 aCTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                             <  5:62681/149‑1 (MQ=255)
                                                                                                                      cAAAACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAg                        <  4:181610/149‑1 (MQ=255)
                                                                                                                           cTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   <  3:302666/149‑1 (MQ=255)
                                                                                                                            tACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   >  4:117767/1‑148 (MQ=255)
                                                                                                                               ccTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                >  8:289876/1‑148 (MQ=255)
                                                                                                                                 tttCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc             >  3:69879/1‑149 (MQ=255)
                                                                                                                                            gAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                  <  2:11985/85‑1 (MQ=255)
                                                                                                                                            gAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                  >  1:11985/1‑85 (MQ=255)
                                                                                                                                            gAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  >  4:316038/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307721‑308008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑