Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	61,260:1	+G	10.0%	coding (430/885 nt)	PP_0052 →	beta‑lactamase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	61,260	1	.	G	10.0%	141.7 / 11.6	40	coding (430/885 nt)	PP_0052	beta‑lactamase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (20/16);  new base G (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTAC  >  NC_002947/61114‑61409                                                                                                                                                    |                                                                                                                                                      cAATTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑Tg                                                                                                                                                     >  3:321431/1‑149 (MQ=255)  aaTTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGc                                                                                                                                                    <  1:72101/149‑1 (MQ=255)    ttGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGca                                                                                                                                                   >  4:171135/1‑148 (MQ=255)       gcgcgcACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGAAGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTCCGT‑TGCACCCg                                                                                                                                               >  8:114603/1‑149 (MQ=255)             acaTTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTggg                                                                                                                                         <  5:48811/149‑1 (MQ=255)               aTTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGca                                                                                                                                       >  6:96709/1‑149 (MQ=255)                ggcatcggccccggggaagtcgcagcgggcggtgccatagtcgggcatgaacaaggtgtctccaacgaacaccgcgatctcgccggcatcttcgatcatgaaactcatgcacgcgggtgtgtGCCCGTGCGT‑TGCACACGCCTGGGcac                                                                                                                                      >  7:137451‑M1/123‑149 (MQ=255)                           cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGt                                                                                                                            <  5:88367/148‑1 (MQ=255)                           cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTg                                                                                                                           >  5:46313/1‑149 (MQ=255)                           cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTg                                                                                                                           >  5:51510/1‑149 (MQ=255)                               aaGTGGTTGGCGCCTTGTTCAACGCAGAGCACGGCTTCGCTCTTGATGGCAGCCAGTTCGATGTGCTTCTCGAAGACGAGGAAGGTTTCATGATTTGGAAACTTCAGTCCCGTGCTT‑TGCAGTCGCCTTGGCACACACGCGGGTGCAt                                                                                                                        <  4:191496/148‑1 (MQ=255)                                           ccTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATc                                                                                                           >  6:71645/1‑149 (MQ=255)                                               gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                                        <  6:46313/148‑1 (MQ=255)                                               gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                                        <  5:96709/148‑1 (MQ=255)                                               gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                                        <  6:51510/148‑1 (MQ=255)                                                ttCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATc                                                                                                           <  4:71174/144‑1 (MQ=255)                                                ttCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGagat                                                                                                      >  3:71174/1‑146 (MQ=255)                                                  cAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGAt                                                                                                     <  3:171135/148‑1 (MQ=255)                                                                gCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                                      <  2:310839/149‑1 (MQ=255)                                                                    cGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGgt                                                                                    <  4:275658/147‑1 (MQ=255)                                                                       ccGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCg                                                                               >  5:226681/1‑149 (MQ=255)                                                                         gTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATc                                                                                         >  1:41507/1‑137 (MQ=255)                                                                         gTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATc                                                                                         <  2:41507/137‑1 (MQ=255)                                                                          tGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACTCACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTg                                                                            <  4:321431/149‑1 (MQ=255)                                                                            aTGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGga                                                                          <  2:131860/149‑1 (MQ=255)                                                                             tGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCC‑GGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATAGCGGTGTTCGTTGgaga                                                                        >  3:35350/1‑149 (MQ=255)                                                                                    cAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCtt                                                                  >  8:14773/1‑149 (MQ=255)                                                                                      gTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGt‑t                                                                                                                                                      <  8:167865/63‑1 (MQ=255)                                                                                      gTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGt‑t                                                                                                                                                      >  7:167865/1‑63 (MQ=255)                                                                                         cGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTc                                                              <  6:160457/148‑1 (MQ=255)                                                                                                      gAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTAt                                                  <  5:71645/147‑1 (MQ=255)                                                                                                      gAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATgg                                                >  7:311041/1‑149 (MQ=255)                                                                                                               gAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCAccgcccg                                       >  4:198833/1‑149 (MQ=255)                                                                                                                    tttCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCg                                  >  4:32935/1‑149 (MQ=255)                                                                                                                               gCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCggg                       >  3:96665/1‑149 (MQ=255)                                                                                                                               gCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCggg                       >  3:27011/1‑149 (MQ=255)                                                                                                                                gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                                      >  6:185936‑M1/11‑85 (MQ=255)                                                                                                                                gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                                      <  5:185936‑M1/75‑1 (MQ=255)                                                                                                                                gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgc                                                                                       >  8:210194‑M1/11‑84 (MQ=255)                                                                                                                                gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgc                                                                                       <  7:210194‑M1/74‑1 (MQ=255)                                                                                                                                cAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCgggg                      >  1:197529/1‑149 (MQ=255)                                                                                                                                                 cGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATg                                                 >  8:137701/1‑105 (MQ=255)                                                                                                                                                   t‑tGCACACGCC‑GGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATAGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTAc  <  4:35350/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CAATTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGGCCGATGCCCGCACCTTGTAC  >  NC_002947/61114‑61409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑