| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 93.1% | 89.5 / 0.7 | 29 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (16/11); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932491‑1932779 | tGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑c < 5:59410/149‑1 (MQ=255) agGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAg < 3:288109/149‑1 (MQ=255) aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATACGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAgg > 5:281206/1‑147 (MQ=255) tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAg > 1:138324/1‑149 (MQ=255) cACGAAAATAGCAGTAAACGCTGGGGCGCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATcgctga > 1:192197/1‑144 (MQ=37) aTACCAGTAACCGCTGGGGC‑CGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAACGTATATGCGCCCCCTGTAGGCGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCGCTGATCCgcgta < 2:192197/149‑5 (MQ=25) tAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATACGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 6:281206/149‑1 (MQ=255) gCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg > 8:84310/1‑149 (MQ=255) ccTTCGCGGGCAAGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCCGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTg > 8:97279/1‑149 (MQ=18) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGcc < 3:176750/142‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGcc > 4:176750/1‑142 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 8:8476/108‑1 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg > 7:8476/1‑108 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 2:273684/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 2:209318/1‑149 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 3:152814/1‑141 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 4:152814/141‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCTACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 2:126946/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 2:175367/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 7:111531/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGCCTTCC‑CC‑GCGAAGGGCTGCAAGCCAGCCCCAAATTGTCGACTACGCGCAACAGCGATCCGGCAGGGTGATCTTCAg > 5:300418/1‑105 (MQ=37) gATATGCTTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCTAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc < 1:209318/149‑1 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt < 6:98986/116‑1 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt > 5:98986/1‑116 (MQ=255) tGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg > 6:330531/1‑149 (MQ=255) gTCCCCTGTAGGAGAGGGCTT‑G‑CCAGCGAAGGGCTGCAACGCAGACCAAAATTGTCTACTACGCGGATCAGCGATCCCGCAGGGTGATCTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGttc > 2:244749/1‑148 (MQ=255) cccTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCTCATCATCGTTGcc < 7:84310/148‑1 (MQ=255) tAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAg < 4:244359/74‑1 (MQ=255) tAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAg > 3:244359/1‑74 (MQ=255) gggCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat < 6:60459/149‑1 (MQ=255) | TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGC‑CGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932491‑1932779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |