Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,385,797:1	+A	6.8%	coding (81/576 nt)	PP_2093 ←	two‑component system response regulator NasT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,385,797	1	.	A	6.8%	348.5 / 18.9	88	coding (81/576 nt)	PP_2093	two‑component system response regulator NasT
Reads supporting (aligned to +/- strand):  ref base . (43/39);  new base A (3/3);  total (46/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

GCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGC  >  NC_002947/2385655‑2385944                                                                                                                                                |                                                                                                                                                      gCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAg                                                                                                                                                 >  8:237365/1‑149 (MQ=255) gCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGGCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAg                                                                                                                                                 >  8:73846/1‑149 (MQ=255)   cgcgGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGTTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GATCATCGAAGcc                                                                                                                                               <  7:139388/149‑1 (MQ=255)   cgcgGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GATCATCGAAGcc                                                                                                                                               <  3:49834/149‑1 (MQ=255)    gcgGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTAGCGATCAGCACCACGCCCGGGCGCACCGTTGCGACGCTAGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCg                                                                                                                                              <  1:263885/149‑1 (MQ=255)           gTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCTCACCGTTTCGACGCAGGCGGGCAGGTCGATCGTCAGGTCTGGGGCTTCGTTGATC‑TCGAAGCCGGccaccc                                                                                                                                       >  2:257321/1‑145 (MQ=255)           gTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCctcac                                                                                                                                       >  8:25897/1‑149 (MQ=255)            tCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCctcact                                                                                                                                      >  8:333632/1‑149 (MQ=255)                ggCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAAc                                                                                                                                  >  6:35853/1‑149 (MQ=255)                ggCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGGACGGGCGCACCGTTACGACGCAGGCGGCCAGGTCGATGGGCAGGTCTGGGGCGTCGAGGATC‑TCGAAGCCGGCCGCGCTCCAc                                                                                                                                  >  8:93996/1‑149 (MQ=255)                     aCCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑Ag                                                                                                                             <  6:111648/148‑1 (MQ=255)                       cAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGccc                                                                                                                          >  2:238921/1‑149 (MQ=255)                       cAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGccc                                                                                                                          >  3:97798/1‑149 (MQ=255)                         gCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCt                                                                                                                         >  7:259765/1‑148 (MQ=255)                         gCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTc                                                                                                                        <  7:237365/149‑1 (MQ=255)                          cACTACCTGTTCCATCACATGGCTGGCTGGCGAGTCGGTATCGTTCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCa                                                                                                                       <  3:184514/149‑1 (MQ=255)                             tACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGcc                                                                                                                                           >  1:100925/1‑127 (MQ=255)                             tACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGcc                                                                                                                                           <  2:100925/127‑1 (MQ=255)                                cTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGAc                                                                                                                 <  7:73846/149‑1 (MQ=255)                                cTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGAc                                                                                                                 >  1:328201/1‑149 (MQ=255)                                   ttCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GATCATCGAAGcccca                                                                                                                                            >  8:219934/1‑117 (MQ=255)                                   ttCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GATCATCGAAGcccca                                                                                                                                            <  7:219934/120‑4 (MQ=255)                                     ccATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGc                                                                                                           >  6:133221/1‑149 (MQ=255)                                        tCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTg                                                                                                        >  7:32150/1‑149 (MQ=255)                                           caTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGctc                                                                                                     >  6:268996/1‑149 (MQ=255)                                            aTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGctct                                                                                                    >  8:13710/1‑149 (MQ=255)                                            aTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGctct                                                                                                    <  4:97798/149‑1 (MQ=255)                                             tCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTg                                                                                                   <  4:27149/149‑1 (MQ=255)                                              cgcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  >  8:295196/1‑149 (MQ=255)                                              cgcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  >  8:290234/1‑149 (MQ=255)                                               gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  <  3:114620/148‑1 (MQ=255)                                               gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  <  2:328201/148‑1 (MQ=255)                                               gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  <  4:109742/148‑1 (MQ=255)                                               gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  >  4:114620/1‑148 (MQ=255)                                               gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTgg                                                                                                  <  4:141361/148‑1 (MQ=255)                                                  gTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGctct                                                                                                    >  3:67476/1‑143 (MQ=255)                                                  gTCTGGCGAGTCGGTATCGATCAGCACCACGGCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGctct                                                                                                    <  4:67476/143‑1 (MQ=255)                                                       gCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTgtcgt                                                                                           <  7:333632/147‑1 (MQ=255)                                                        cGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGtcgtcg                                                                                         >  7:300775/1‑148 (MQ=255)                                                         gAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGaa                                                                                                                                                  >  1:303473/1‑92 (MQ=255)                                                         gAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGaa                                                                                                                                                  <  2:303473/92‑1 (MQ=255)                                                          aGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGAt                                                                                       >  8:159187/1‑148 (MQ=255)                                                           gTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATca                                                                                     <  5:268996/149‑1 (MQ=255)                                                           gTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGCCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATca                                                                                     >  2:291727/1‑149 (MQ=255)                                                            tCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTgtcgtc                                                                                          >  1:157451/1‑143 (MQ=255)                                                            tCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTgtcgtc                                                                                          <  2:157451/143‑1 (MQ=255)                                                               gTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCTATCGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTgtc                                                                                             >  5:250330/1‑137 (MQ=255)                                                                     aTCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGTCGACCG‑AGCTTGATCTGGGTGTCGTCGATCAGCAGGATgcg                                                                           >  3:221877/1‑149 (MQ=255)                                                                       cAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGTATgcgc                                                                          <  1:257321/148‑1 (MQ=255)                                                                        aGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCGCAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATgcgc                                                                          <  1:238921/148‑1 (MQ=255)                                                                        aGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                        <  7:290234/149‑1 (MQ=255)                                                                        aGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                        <  7:295196/149‑1 (MQ=255)                                                                          caccacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGACAGGTCTGGGGCTTGGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGCTGTCGTCGCTCAGCAGGATGCGCAACa                                                                      >  8:149581/1‑149 (MQ=255)                                                                               cGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                 <  5:35853/149‑1 (MQ=255)                                                                               cGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGACC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                 <  6:324780/149‑1 (MQ=255)                                                                                gTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                 <  5:314675/148‑1 (MQ=255)                                                                                gTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                 >  6:314675/1‑148 (MQ=255)                                                                                    gggCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTccc                                                            <  8:259765/149‑1 (MQ=255)                                                                                    gggCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTccc                                                            <  3:66278/149‑1 (MQ=255)                                                                                      gcgcACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTcccc                                                           >  6:224085/1‑148 (MQ=255)                                                                                                   aCGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGaca                                             <  7:159187/149‑1 (MQ=255)                                                                                                   aCGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGaca                                             >  7:238534/1‑149 (MQ=255)                                                                                                     gCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACg                                           <  7:25897/149‑1 (MQ=255)                                                                                                      cAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACgg                                          <  7:320611/149‑1 (MQ=255)                                                                                                      cAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCACTCAGGCGACCG‑AGCTTGCTCAGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTAGTCCCCCTCACGCGcc                                                 >  3:220662/1‑142 (MQ=255)                                                                                                        ggCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGTGCAACATGTTCGTccccc                                                          <  6:66827/131‑1 (MQ=255)                                                                                                            ggCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCTCTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCg                                    <  8:300775/149‑1 (MQ=255)                                                                                                                 gTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCaa                               >  1:23572/1‑149 (MQ=255)                                                                                                                  tCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAAc                              >  4:202054/1‑149 (MQ=255)                                                                                                                   cGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACt                             >  7:259896/1‑149 (MQ=255)                                                                                                                    gATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                        >  4:135608/1‑105 (MQ=255)                                                                                                                    gATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                        <  3:135608/105‑1 (MQ=255)                                                                                                                      tGGTCAGGTCTGGGGCTTAGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGGCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGc                          <  7:149581/149‑1 (MQ=255)                                                                                                                            ggTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAgg                    <  5:133221/149‑1 (MQ=255)                                                                                                                             gTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCTAGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATcagca                                                                                  >  1:248658/1‑87 (MQ=255)                                                                                                                             gTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATcagca                                                                                  <  2:248658/86‑1 (MQ=255)                                                                                                                             gTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAgg                    >  6:142922/1‑148 (MQ=255)                                                                                                                               cTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAgg                    >  4:295897/1‑146 (MQ=255)                                                                                                                               cTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAgg                    <  3:295897/146‑1 (MQ=255)                                                                                                                                  cggCTTCGA‑GATCATCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCCCGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGc               >  2:102791/2‑148 (MQ=255)                                                                                                                                    gCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAg            <  1:291727/149‑1 (MQ=255)                                                                                                                                    gCTTCGA‑GATCATCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAg            >  1:323264/1‑149 (MQ=255)                                                                                                                                      ttCGATGATC‑TCGAAGCCGGCCTCACTCATCGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCCTCGATCAGCAGGATGCGAAACATGTTCGTCCCCCTCAGGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGc               <  7:299443/144‑1 (MQ=255)                                                                                                                                      ttCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGGATCCGGCAAGTCGCCCAAGGCAAGCAAGCt          >  2:142353/1‑149 (MQ=255)                                                                                                                                      ttCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCt          >  7:127754/1‑149 (MQ=255)                                                                                                                                      ttCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCACCTCACGCGCCGACACGCGCAACGGGCAACTCGCCCAACGCATGCAAga           >  8:299443/1‑147 (MQ=255)                                                                                                                                        cGATGATC‑TCGAAGCCGGCCTCGCTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTg         >  7:55187/1‑148 (MQ=255)                                                                                                                                        cGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTg         <  2:23572/148‑1 (MQ=255)                                                                                                                                              tc‑tcGAAGCCGGCCTCACTCAACGC‑AGACCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGc  >  4:66980/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                      GCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGC‑AGCCCTCAGGCGACCG‑AGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGC  >  NC_002947/2385655‑2385944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑