Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,436,189:1	+A	6.5%	coding (124/813 nt)	gpI →	tail formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,436,189	1	.	A	6.5%	253.8 / 11.4	62	coding (124/813 nt)	gpI	tail formation protein
Reads supporting (aligned to +/- strand):  ref base . (27/31);  new base A (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

TACGCTGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGC  >  NC_002947/3436042‑3436331                                                                                                                                                     |                                                                                                                                               tACGCTGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑t                                                                                                                                               >  5:163954/1‑149 (MQ=255)   cGCTGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGc                                                                                                                                             >  6:196016/1‑149 (MQ=255)        aCGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGCGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCct                                                                                                                                          <  1:14810/147‑1 (MQ=255)        aCGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctgg                                                                                                                                        <  6:163954/149‑1 (MQ=255)             gAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggctgg                                                                                                                                    <  4:11314/148‑1 (MQ=255)             gAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCtggctggc                                                                                                                                   <  3:298356/149‑1 (MQ=255)                       aCATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCAGCCC‑TCGACCTTCCATGCCCAGGCCAGCCAGGGCagga                                                                                                                         <  5:319179/149‑4 (MQ=37)                         aTGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATgg                                                                                                                         >  7:132172/1‑147 (MQ=255)                                 gTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggctgg                                                                                                                                    <  8:319054/128‑1 (MQ=255)                                 gTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggctgg                                                                                                                                    >  7:319054/1‑128 (MQ=255)                                    aCCGCACAACACCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCt                                                                                                            >  6:355225/1‑149 (MQ=255)                                      cGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTgg                                                                                                          <  2:256691/149‑1 (MQ=255)                                      cGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTCGGCATGGAAGGTCGAGGGCTgg                                                                                                          >  7:152688/1‑149 (MQ=255)                                           aaCGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                     >  8:92122/1‑149 (MQ=255)                                           aaCGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                     <  7:201739/149‑1 (MQ=255)                                              gCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcggc                                                                                                  <  2:331140/149‑1 (MQ=255)                                                cACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCt                                                                                                 >  6:92390/1‑148 (MQ=255)                                                  cccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTa                                                                                               >  6:197777/1‑148 (MQ=255)                                                   ccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTaca                                                                                             <  8:132172/149‑1 (MQ=255)                                                   ccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTaca                                                                                             <  5:92390/149‑1 (MQ=255)                                                   ccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTaca                                                                                             >  1:338598/1‑149 (MQ=255)                                                          gAGCGCGCCCTGGAGGTGGCCGACGACCTTGGGCTTGAGCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGGGCTGGCTGGGGTGGGCATGGAAGGTCGTGGGCTGGGAGGCGGCt                                                                                                 <  8:184519/138‑1 (MQ=255)                                                          gAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATACGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCAAGGAAGGTCGAGGGCTGGGAGGCGGCt                                                                                                 >  7:184519/1‑138 (MQ=255)                                                            gcgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGAcc                                                                                    >  2:133000/1‑149 (MQ=255)                                                                  ccTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgcc                                                                              <  5:355225/149‑1 (MQ=255)                                                                         gcggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCt                                                                       >  6:70162/1‑149 (MQ=255)                                                                            gccgccGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTg                                                                                                                                >  3:289747/1‑89 (MQ=255)                                                                            gccgccGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTg                                                                                                                                <  4:289747/89‑1 (MQ=255)                                                                              cgccgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATcc                                                                  >  6:121314/1‑149 (MQ=255)                                                                                 cgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCcgc                                                                >  6:232090/1‑148 (MQ=255)                                                                                  gACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                              <  1:136321/149‑1 (MQ=255)                                                                                   aCCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                             <  7:92122/149‑1 (MQ=255)                                                                                          ccTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTc                                                      >  5:246400/1‑149 (MQ=255)                                                                                              gACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCg                                                    <  5:232090/147‑1 (MQ=255)                                                                                               aCCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                 <  1:133000/149‑1 (MQ=255)                                                                                               aCCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGt                                                  <  5:70162/148‑1 (MQ=255)                                                                                                  cGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                              <  5:197777/149‑1 (MQ=255)                                                                                                  cGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                              <  5:196016/149‑1 (MQ=255)                                                                                                  cGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                              <  6:246400/149‑1 (MQ=255)                                                                                                  cGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTca                                               >  6:337837/1‑148 (MQ=255)                                                                                                       aTTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGAc                                         >  7:105605/1‑149 (MQ=255)                                                                                                       aTTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGAc                                         >  8:49438/1‑149 (MQ=255)                                                                                                           tCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCgcgcgc                                                                        <  1:82390/114‑1 (MQ=255)                                                                                                           tCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCgcgcgc                                                                        >  2:82390/1‑114 (MQ=255)                                                                                                                  cGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACgg                              <  5:121314/149‑1 (MQ=255)                                                                                                                      gCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCg                           <  7:49438/148‑1 (MQ=255)                                                                                                                         gACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGgcgc                       <  8:105605/149‑1 (MQ=255)                                                                                                                               tCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGTGCGATTcg                 >  7:163851/1‑149 (MQ=255)                                                                                                                               tCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcg                 >  1:35385/1‑149 (MQ=255)                                                                                                                                 aCGCTGCCCGCCGAACTTCC‑TGCCATGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCAtt                                                       >  5:97226/1‑109 (MQ=255)                                                                                                                                 aCGCTGCCCGCCGAACTTCC‑TGCCATGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCAtt                                                       <  6:97226/109‑1 (MQ=255)                                                                                                                                   gCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                             >  6:356191/1‑101 (MQ=255)                                                                                                                                   gCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                             <  5:356191/101‑1 (MQ=255)                                                                                                                                   gCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcgg             >  2:315238/1‑149 (MQ=255)                                                                                                                                     tGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcgg             >  3:131811/1‑147 (MQ=255)                                                                                                                                     tGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcgg             <  4:131811/147‑1 (MQ=255)                                                                                                                                         cctcgACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGa                     >  5:345476/4‑135 (MQ=25)                                                                                                                                         cctcgACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGa                     <  6:345476/132‑1 (MQ=25)                                                                                                                                          ctcgACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACAAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTc       >  7:252213/3‑148 (MQ=37)                                                                                                                                             gAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAgg   <  2:35385/149‑1 (MQ=255)                                                                                                                                              aaCTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGc  <  1:315238/149‑1 (MQ=255)                                                                                                                                                 ttCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGt           <  3:311584/137‑1 (MQ=255)                                                                                                                                                 ttCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGt           >  4:311584/1‑137 (MQ=255)                                                                                                                                                     |                                                                                                                                               TACGCTGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGC  >  NC_002947/3436042‑3436331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑